Checking Confluency of Cells

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Biology Basics

Biology Basics

Күн бұрын

Пікірлер: 4
@macadance92
@macadance92 3 жыл бұрын
thanks so much for this video. I am a 1st year PhD student and I was struggling to see the difference as all my cells were either happy or not happy
@biologybasics
@biologybasics 3 жыл бұрын
Glad to know I could help. It definitely takes a little while before this stuff becomes intuitive. Don't worry before you know it, you'll be "one with the cells" you work with :)
@ivanrubalcava08
@ivanrubalcava08 2 жыл бұрын
I feel this is very easier to see with adherent cell cultures. Does the same approach apply for freely suspended cells cultures?
@biologybasics
@biologybasics 2 жыл бұрын
For suspended cultures we typically look at increase in turbidity of the culture either visually or through spectrophotometry. Suspended cultures are considered over confluent and no longer in the log phase of growth typically with absorbance above 1 or because cells in the culture begin to clump together and float on top of the culture.
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