Flow cytometry is a popular laser-based technology to analyze the characteristics of cells or particles. Watch our flow cytometry video protocol that teaches you how to prepare and stain your cells for flow cytometry analysis.
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Timestamps:
00:00 Introduction
00:13 What you will need
00:41Titration (optional)
01:00 Preparing a primary antibody solution
01:15 Re-suspending cells and measuring cell viability
01:48 Centrifugation
02:03 Blocking step
02:37 Staining with primary antibody
03:51 Secondary antibody step (optional)
04:55 Checking for live/dead cells
05:16 Storing the cell suspension