DNA cloning

Рет қаралды 820,360

11 жыл бұрын

DNA cloning animation - This lecture explains about the DNA cloning techniques with vectors. The molecular mechanism of DNA cloning is explained with animation.
shomusbiology.com/
Source: Lodish, et al., Molecular Cell Biology, Fifth Edition, W. H. Freeman & Co.
© 2004 W. H. Freeman & Co., and Sumanas, Inc.
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Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms.The use of the word cloning refers to the fact that the method involves the replication of a single DNA molecule starting from a single living cell to generate a large population of cells containing identical DNA molecules. Molecular cloning generally uses DNA sequences from two different organisms: the species that is the source of the DNA to be cloned, and the species that will serve as the living host for replication of the recombinant DNA. Molecular cloning methods are central to many contemporary areas of modern biology and medicine.
In a conventional molecular cloning experiment, the DNA to be cloned is obtained from an organism of interest, then treated with enzymes in the test tube to generate smaller DNA fragments. Subsequently, these fragments are then combined with vector DNA to generate recombinant DNA molecules. The recombinant DNA is then introduced into a host organism (typically an easy-to-grow, benign, laboratory strain of E. coli bacteria). This will generate a population of organisms in which recombinant DNA molecules are replicated along with the host DNA. Because they contain foreign DNA fragments, these are transgenic or genetically-modified microorganisms (GMO).
Link- en.wikipedia.org/wiki/Main_Page

Пікірлер: 177

@basantalsayed3127 7 жыл бұрын

this video explains in 4 minutes what my professor couldn't in 1:47:34 of awkward lecture officially Shomu's Biology Is one of my references

@shomusbiologyofficial 7 жыл бұрын

+Basant Alsayed thank you. Glad you liked my lectures

@yellow8206 5 жыл бұрын

Big facts professors can be dumbasses.

@zaara8151 4 жыл бұрын

🤣🤣🤣same here

@mustafasadig2390 Жыл бұрын

like literally!

@Hoid. 7 жыл бұрын

Thank you so much! This was so clear and understandable, my teacher spent 2 hours on this and managed to confuse me more than anything

@ayasamer205 Жыл бұрын

10 years ago and still very beneficial and amazing job Thank u soo much.. u made really alot for us❤

@shomusbiologyofficial Жыл бұрын

All the best

@iMusikkForeva 2 жыл бұрын

Shomu's Biology- words cannot describe how helpful you have been in my undergrad so far as a Bio major. Thank you soooo much

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@kathyweeds 7 жыл бұрын

Thank you! I could not visualize the technique for my exam. Pics were not enough. Your moving example is perfect. Keep it up!

@shomusbiologyofficial 7 жыл бұрын

+Kathy Eich thank you.

@harshpreetsingh4368 7 жыл бұрын

Kathy Eich '

@suraal-bahadli2013 7 жыл бұрын

Shomu's Biology )

@maryamnaseer2897 6 жыл бұрын

can u plz email the book "essentials of cell and molecular biology by V.Mlathi...plzzz

@musicfever401 5 жыл бұрын

Yes i totally agree with you.

@ily2PM 6 жыл бұрын

simple and to the point!! love it!

@jvicism7135 8 жыл бұрын

Thank you so much for the video. It really helps put everything I've read for class together.

@Pratapsingh0007 7 жыл бұрын

thanks Bhai....sabse mushkil topic h ye book ka....but from now it become easy.......keep going...

@ItsLogic 4 жыл бұрын

Remind me to come back to this in exactly a year when I have my next bio chem midterms

@324shine 3 жыл бұрын

hey it's almost been a year

@generalbugis5700 3 жыл бұрын

Bro how've you been

@ItsLogic 3 жыл бұрын

@@generalbugis5700 chem midterms are coming up soon-ish

@ItsLogic 3 жыл бұрын

@@324shine chem midterms on their way

@generalbugis5700 3 жыл бұрын

@@ItsLogic good luck and may the forces be with you!

@iboodzZ 9 жыл бұрын

woow best video to explain many topics at once

@meowismeify 9 жыл бұрын

Thank you!! This helped!

@anas-xp1vg 5 жыл бұрын

Best biology channel on KZfaq.

@shomusbiologyofficial 5 жыл бұрын

Thank you. Glad you liked my lectures

@laraelzaher1914 7 жыл бұрын

Thanks a lot u helped us ❤️❤️

@MetoMailey 11 жыл бұрын

I got it now .. Easy & Simple ♥

@boikoketsoramokolo7616 6 жыл бұрын

wow this was very helpful... Thank u very much

@sahilmakkar6699 8 жыл бұрын

very helpful videos ....thnx....god bless u

@nadeemnawaz6859 6 жыл бұрын

WoW! Fabulous

@sijowdabdo8421 9 жыл бұрын

Thanks for helpfull information

@taaptee 4 жыл бұрын

this is beyond helpful, thank you so much

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@mushtaqhussain6121 6 жыл бұрын

Nice lectures.... With digramic process

@sahoogoutam 7 жыл бұрын

able to understand very well

@aakritipandey4952 8 жыл бұрын

Thank you so much! This video gave me a lot of clarity :)

@bambinarm4071 10 жыл бұрын

This was good!

@jacquelinekang75 6 жыл бұрын

Thank you very much. This video is very helpful!

@shomusbiologyofficial 6 жыл бұрын

+Jacqueline you're welcome

@williamjayaraj2244 2 жыл бұрын

Thanks for this video.

@1987minimum 8 жыл бұрын

thanks a lot ,,being an homemaker I cud reach my exam needs , these videos hv helped me a lot , please do help me wit few videos concerning population genetics ..I'm going to take up my csir net ...

@shomusbiologyofficial 8 жыл бұрын

There are videos on population genetics in my channel. You can find it easily.

@1987minimum 8 жыл бұрын

Got it, thank u

@chandnisingh8707 5 жыл бұрын

Thanks 💚🇮🇳

@hassankhormi9076 8 жыл бұрын

thank you so much

@tessgallagher9738 10 жыл бұрын

so could i do the same thing with dinosaur or over all animal dna or is do I ave to it it a different way?

@Dhruba_Banerjee 10 жыл бұрын

Thank You very much. Helped me to understand the significance of selectable markers. Please prepare a separate video on selectable markers & cloning site too for me at least. I am giving XII boards in 2014. Urgently need it. Thank You.

@shomusbiologyofficial 10 жыл бұрын

You will find those topic under the "cloning vector" video.Watch those video.

@Dhruba_Banerjee 10 жыл бұрын

Thanks

@adt_10terrarian5 4 жыл бұрын

"The Zeta Reticulians want to know your location" (Great video by the way )

@maltisaini7584 5 жыл бұрын

Very helpful sir thanks for making this

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@sobster123 8 жыл бұрын

really really good.! :)

@hjraoy 10 жыл бұрын

thank You very much. Helped me to understand DNA cloning

@sm247 4 жыл бұрын

and you dont now before that video? يعني انت كنت لاتعرف قبل هذا الفيديو ؟

@dishninuwandika4907 Жыл бұрын

Thank you so much for this wonderful vedio. It's very important and useful for our studies

@shomusbiologyofficial Жыл бұрын

You're welcome

@bhagwatbhattarai705 7 жыл бұрын

great video

@mahamhaq1294 6 жыл бұрын

which is a suitable vector to be incorporated with a large external DNA fragment? Small or large?

@AhmedMD 9 жыл бұрын

Thanks.

@suxo774 2 жыл бұрын

This is the best video, no cap

@shomusbiologyofficial 2 жыл бұрын

Thank you

@hardikgohil4190 7 жыл бұрын

thank you

@user-gg5jz7ct8n 7 ай бұрын

is must the restrictions enzymes to cut dna at G and A not other site??

@ayushsinha2643 5 жыл бұрын

Your videos are really very helpfull!! But please try not to show pop-ups while the video is playing. It covers the screen and is very distracting!!!

@kerryvp 11 жыл бұрын

Gracias

@chandnisingh8707 5 жыл бұрын

God bless you 😇

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@amalhosny 7 жыл бұрын

great

@shafiyahasan3275 2 жыл бұрын

Helpful video sir.. thanku somuchhhh... please upload more such videos 👍

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@shafiyahasan3275 2 жыл бұрын

@@shomusbiologyofficial you're welcome sir...keep it up👍

@64.sreejithreju2 4 жыл бұрын

Brilliant sir

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@_royal.girl_3084 3 жыл бұрын

It help ful for me.... thanku !!

@shomusbiologyofficial 3 жыл бұрын

You're welcome

@_royal.girl_3084 3 жыл бұрын

😊

@miss.beeslibrary220 6 жыл бұрын

Sir pls can you make a video on trade secret laws of human cloning 😪 even their is no data on net.

@altair2594 10 жыл бұрын

smart!

@uzmashaikh1007 4 жыл бұрын

Nice video.. Its really very helpful video...

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@medicinestudent8349 Жыл бұрын

Thank you!

@shomusbiologyofficial Жыл бұрын

You're welcome

@gtagamer757757 7 жыл бұрын

so if we got a fragment of dinosaur dna and put it into a plasmids it could multiply and we could put it together and form a dinosaur?

@nathalie7833 7 жыл бұрын

Where would you get a usable dinosaur DNA?

@wolves44445 7 жыл бұрын

Well we don't have any dinosaur DNA, unfortunately

@gtagamer757757 7 жыл бұрын

well they have found fragments of dinosaur DNA in fossils

@shwetakhade6531 6 жыл бұрын

Yeah.. That's the principle they showed in the movies like Jurassic Park and Jurassic World.

@anirbanghosal8427 6 жыл бұрын

we just can't put it together to make dinosaur just like that...

@LishaBaByDoll 10 жыл бұрын

I get it now. so happy haha

@MrAbuse96 4 жыл бұрын

Very clear thanks

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@sababashir12 2 жыл бұрын

Very helpful 👍

@shomusbiologyofficial 2 жыл бұрын

Thank you

@sheefaali7186 3 ай бұрын

Which book should I follow for RDT?

@jacksonmace9566 2 жыл бұрын

OG Shomu

@anilmeena_02 4 жыл бұрын

Awesome video 👍

@shomusbiologyofficial 4 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@natalieb.923 3 жыл бұрын

So if there are many plasmids in the solution with sticky ends, what prevents the plasmids from recombining with itself for another plasmid instead of the DNA fragment you are trying to insert?

@ppwithaweewee9403 3 жыл бұрын

For now we go with we simply don't know. But one possibility is it might be happening but there are so many available and wayyyyy more genes available quickly, so the second the sticky ends are exposed BOOM! they go and hug. Tbh it's practically possible like science AIN'T clean in lab, like it's Portraited on paper stuff like this can happen. And there are countermeasures too. Hopefully i cleared your doubt.

@taimoorakhter2396 4 жыл бұрын

Clone of specific gene, is it are same thing to DNA cloning??

@babbirajan1867 4 жыл бұрын

My teacher is teaching still now but I didn't understood.... But you make it clear

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@imjohnsteve2715 4 жыл бұрын

Lol IAM grade 10 student even though we don't have this lesson in GRADE 10 IAM using this for research paper lol😂😂👍

@shomusbiologyofficial 4 жыл бұрын

Great

@spanditaroy9973 5 жыл бұрын

Just wow

@shomusbiologyofficial 5 жыл бұрын

Thank you. Glad you liked my lectures

@ghadeeralmlek6394 5 жыл бұрын

Thanks alot.. Can you maka a tutorial video about cell banking plz..

@miguelramirez187 5 жыл бұрын

Thank you

@shomusbiologyofficial 5 жыл бұрын

You're welcome

@boobas8127 3 жыл бұрын

am i the only one who is fascinated by the concept?

@ppwithaweewee9403 3 жыл бұрын

Trust this is magic for me

@anshulrana5614 Жыл бұрын

Thanks sir

@shomusbiologyofficial Жыл бұрын

You're welcome

@madeline_parks 8 жыл бұрын

How do you determine which bacterial colonies have the gene of interest? Also, if you wanted only exons and no introns, wouldn't you use RNA and reverse transcription to produce complimentary DNA? Let's say you want to genetically modify an embryonic animal: Would you inject the bacteria themselves into the embryo or would you need to isolate plasmid DNA?

@nathalie7833 7 жыл бұрын

He said it, through doing an assay on each colony (for your first question). The bacteria is the host, if you want to genetically modify an embryonic animal, wouldn't the animal be your host? So you don't inject the bacteria, you inject the plasmid into a still growing zygote (okay I think doing it in a multicellular animal would be very much more difficult?)

@enajlopez733 8 жыл бұрын

how to download this?

@manar-nasser 4 жыл бұрын

thank u sooo mutch

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@ddobrik6596 3 жыл бұрын

it is complicated science

@proloyghosh. 4 жыл бұрын

Please suggest a book for biotechnology so that I build up my concept regarding this. Please sir

@HajSlapaHoe 4 жыл бұрын

does anybody have a summary from this?

@cinthialara386 6 ай бұрын

Great video 👍 by the way,you can obviously clone prehistoric and current animals and edit DNA and that is very interesting to me an example would be to clone an arsinoitherium you must alter the elephant's DNA and the mix it with rhinoceros DNA to transform it into a prehistoric animal,dorudon alter the DNA of porpoises to turn them into(prehistorics whales) and finally megalania,a close relative of the komodo dragon alter the DNA of the Komodo dragon to turn it into a prehistoric reptile there is a chance that this will happen?

@Battery-kf4vu 7 жыл бұрын

Would it be possible to add several alleles of the HTERT gene to the DNA of a spermatozoid and an ovum so that the resulting fertilized ovum could produce more telomerase and be immortal?

@popflorian2991 4 жыл бұрын

How the plasmid knows to replicate at the same time with the cell?

@sm247 4 жыл бұрын

حلو | nice | good |

@jasimjasim2639 5 жыл бұрын

could we take any cell of our body.

@ppwithaweewee9403 3 жыл бұрын

Wow 3 hrs to 4 mins.

@khanxercel9756 2 жыл бұрын

I understand in 4 minutes.

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@sanatdas2011 8 жыл бұрын

Sir, thanks a lot for the video. I also need your help in my assignment submissions, I am currently doing my graduation from University of Calcutta, Department of Zoology. In my practical works I have a lot of pressure in third year, cannot really finish it yet. If you can kindly help me out then that would be of great help. Thank you.

@abritanath 7 жыл бұрын

Hi! Can you upload a video for Homopolymer tailing? thanks

@gurubabawithshiwani2433 6 жыл бұрын

PlzzZZZZZZZZZZZZZZZZZZZZZZZZZZZZZZZZZZZZ make another animation for cloning of liver cells

@jdsallinone2292 3 жыл бұрын

Sir amar kichu bisoi janar ache-structure-toxicity relationship,drug design,DNA binding,biological activity of metal ligand complex..ai 4 te tpic er opor video banale khub help hoi..

@harunaadamu9045 8 жыл бұрын

peace love ya'll

@proloyghosh. 4 жыл бұрын

Multiple cloning site helps in incorporation of foreign DNA in biotechnology. But what is the usefulness of that site for bacteria itself??. Dose all plasmid contains this site or it is constructed? Please help me Sir.

@shomusbiologyofficial 4 жыл бұрын

We artificially construct these sites. Generally bacteria has some restriction endonuclease sites in their Plasmid or in chromosomal dna

@proloyghosh. 4 жыл бұрын

@@shomusbiologyofficial okay Sir. Thank you sir, thank you so much.

@laraelzaher1914 7 жыл бұрын

Smooches 😘😘

@adamwo5267 4 жыл бұрын

That intro tho lol

@marianadeau8632 6 жыл бұрын

I'd give it a thumbs up if I didn't have to close the million annotations every other second

@theresepabroa4389 5 жыл бұрын

you can turn the annotations off

@larva_tuba099 Жыл бұрын

@David7hr 8 жыл бұрын

I can,t follow his Indian accent. He should use a narrator and republish these excellent materials!!

@shomusbiologyofficial 8 жыл бұрын

+David C hi. I tried to improve my accent. You can catch my latest videos. Hope you can understand them.

@lanoanhduongthi3536 7 жыл бұрын

At the first stage i could not follow you but now I can follow almost what did you explain, my english also improved from you. very thank to you but could you please speak slowly for others they can follow you

@viratjoshi4780 6 жыл бұрын

What is need of qmplicilline in nutrient agar

@saileshyadav4299 5 жыл бұрын

Sir, which came first,man or women?

@georgiakat4695 6 жыл бұрын

What is a zaime?

@Tony-cf8fd 6 жыл бұрын

it should be enzyme.

@Lazuli99 Жыл бұрын

can we code our dna to be immortal? I want to beat death

@shomusbiologyofficial Жыл бұрын

@claresiatsany5457 4 жыл бұрын

Haloo off c 2017 biologi um

@jontesakify 5 жыл бұрын

Hey is this narrator the same dude as in that the missile knows where it is shit?

@HimanshuRaj-ry2gx 6 жыл бұрын

More like "chomu's biology"....

@standforchrist1980 2 жыл бұрын

Jesus said to her, "I am the resurrection and the life. The one who believes in me will live, even though they die; John 11 25 Believe him, Repent and turn to Him, get Saved, get a ticket to Heaven, HE LOVES YOU, HE WANTS TO SAVE YOU FROM DEATH HELL SIN AND EVERYTHING THAT'S NOT GOOD FOR YOU, HE WANTS TO GIVE YOU A REASON TO LIVE, HE WANTS TO LOVE YOU AS HIS CHILD NOT AS HIS CREATION BUT AS HIS OWN CHILD, BECAUSE OF WHICH HE DIED FOR YOU ON THE CROSS, TO PROVIDE YOU WITH GRACE AND MERCY, FORGIVENESS AND LOVE ETERNAL, NOW REPENT AND TURN TO HIM, RECEIVE HIS GIFT THAT IS SALVATION WHICH IS ONLY THROUGH GRACE NOT BY WORKS, TIME ISN'T OVER YET GATES TO HEAVEN, GATES TO HIS GLORY, HANDS OF HIS LOVE HAVEN'T CLOSED YET, BUT END IS AT THE DOOR, THEREFORE MOVE FORWARD SAY YES TO GOD, CONFESS TO THE LORD AND ASK FOR FORGIVENESS AND SAY HERE I AM LORD, TAKE MY LIFE AND LET IT BE CONSECRATED LORD TO THEE❤❤✝️(Upon you all decisions are left, God bless you