In this video we discuss a type of synthetic nuclease, namely the TALEN, standing for transcription activator-like effector nucleases.
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@payalwankhede13932 ай бұрын
Finally I got it. This is the best explanation available on the internet. Thanks
@navadeepdorai29182 жыл бұрын
Old standard of teaching... A gold standard 👍🏻
@grace96342 Жыл бұрын
our college needs you as our professor, you explained something in 10 minutes which our lecturer failed to present in a 1h lecture. THANK YOU SO MUCH this helped me a lot
@md.golamazom27034 ай бұрын
Great explanation on TALEN I found on KZfaq so far
@MrPelafio6 жыл бұрын
I was just about to google difference between TALEN and ZFN, when at the very end you explained it. Wonderful!
@harryli20537 жыл бұрын
Best explanation ever! Thanks so much for your clear explanations and visual demonstrations!
@monthappy25213 жыл бұрын
Dude, I absolutely love you 💕 Greetings from Colombia.
@talimachet24193 жыл бұрын
You have saved my life - I am eternally grateful. Thank you so much!!!!
@meriamtayar89825 жыл бұрын
You are absolutely amazing. Great explanation! Thank you!
@mayso11047 жыл бұрын
this is the best explanation I've seen. Thanks!
@tereabentley58969 жыл бұрын
Thanks, this is a great explanation. Xanthomonas FOK1
@susanavazquez2244 жыл бұрын
It is excelent explanation and so great it is done the old way of personal teaching, paper pencil and a great teacher! congrats!!
@condimentado7 жыл бұрын
BEST EXPLANATION EVER!!!!
@albertobray69637 жыл бұрын
AWSOME explanation!!! Thank you so much
@paygeshelton62525 жыл бұрын
Thank you so much!!! This was amazing and easy to follow!!!
@ozanatasoy55918 жыл бұрын
Thank you friend. As a virologist it's very simple and informative explanation for me.
@g-host6527 Жыл бұрын
32 min left to study before test, this dude saves my life
@askarrafi2434 жыл бұрын
Thanks for your complete and clear explanation. It was awesome.
@changachangueria7 жыл бұрын
WOW!! nice explanation!! TY so much.. great job
@chutimon.ceefang7 жыл бұрын
Thank you so much this helps me a lot.
@mahachris54778 жыл бұрын
Thanks , you made it look so simple.
@lukapreloznik71234 жыл бұрын
I'd give you a HEART, but only LIKEs are available on yt...very good explanation, thank you very much!!!
@aquamarine36993 жыл бұрын
Thank you for the clear explanation!
@harleyjerram96998 жыл бұрын
Thank you, really nice video!
@jeminmangukiya86553 жыл бұрын
bloody brilliant
@misabella3233 жыл бұрын
Thank you sooo much!!
@pauladalcinmartins74218 жыл бұрын
Great explanation!
@JMmitchell6337 жыл бұрын
Great explanation....thanks!
@sancharan63423 жыл бұрын
Again.. sir you are awesome.
@houmamkitet95554 жыл бұрын
Very nicely explained, thank you very much for making the video
@karamella5653 жыл бұрын
thank you very much.
@angeloorsini17646 жыл бұрын
The best one on youtube. Thanks man!
@sarahbrm2536 жыл бұрын
the best* one ..
@angeloorsini17646 жыл бұрын
thx
@gregorysmith1134 Жыл бұрын
Excellent explanation.
@joannamaw89202 жыл бұрын
Thanku for this!
@sapastje7 жыл бұрын
helped a lot, thx!
@annakassis81756 жыл бұрын
That's the best explanation ever, (Fok1)
@MonsieurDepadieu4 жыл бұрын
Thanks, great explanation clear as Clearacil®
@Nico140719975 жыл бұрын
Take a shot every time he says "transcription activator like effectors"
@christenemelad54355 жыл бұрын
Excellent work
@hoimebanerjee10664 жыл бұрын
Nicely explained. Thanks 😊😊
@XamaL17 жыл бұрын
Thank you !
@Neo-rd7ef7 жыл бұрын
Thank you very much
@mgold99995 жыл бұрын
You could have mentioned the 34 aa repeats in the TALE where aa 12 and 13 are variable and specify the DNA base maybe.
@ayaqz31443 жыл бұрын
thank you
@deblinachakraborty5231 Жыл бұрын
😊😊make more vedios sir, we are in need.
@Christina-eq8yc7 жыл бұрын
THANKS!
@gasperkosmac76728 жыл бұрын
THANK YOU
@Hansel551 Жыл бұрын
Nice.
@celinec6366 Жыл бұрын
Is it true that Fok l cuts 9 nucleotides downstream in the first strand and 13 nucleotides upstream in the other strand? I didn't understand it from your movie.
@serventofalfaandtheomega30403 жыл бұрын
Thanks
@lend2150 Жыл бұрын
Thanks for this video. You simplified it well. May I correct that it is FokI or Fok1 (Fok - One not Fok L) and it is Xanthomonas (not Xanfhromonas, it is t not f).
@paucska4 жыл бұрын
very good explanation, thanks! Although: what are the chances these sequences getting attached to the wrong place? :O I mean there are some chances, aren't there, considering the very lengthy and extensive size of the human genome...
@Samsu7777777 Жыл бұрын
Ultrasonic lecture
@056vatsalapandey32 жыл бұрын
thank you booooooooooooooo
@sudiptasarma3 жыл бұрын
So, on the paper, it says that RVDs of TAL effectors correspond directly to the nucleotides in their target sites, one RVD to one nucleotide..my question is TALeffector are amino acids only, so how can one amino acid recognize one nucleotide? it is generally, three nucleotides represent one amino acid..or correct me if I am wrong please, bit confusing
@aurberdyson19402 жыл бұрын
Uh... I'm curious, but not professional. I've seem many books write Fok"I"(I i) instead of Fok"l" (L l). So, could anybody tell me which is right? I'll be appreciated. :)
@ChengVincentckf9 жыл бұрын
Great explanation, I am not sure that is FokL or Fok I
@ardalanmansouri81768 жыл бұрын
It is actually Fok1 (or capital i as a Greek number instead of "1"). For more information regarding its discovery please go to the following link published by the first researcher discovered it: www.ncbi.nlm.nih.gov/pubmed/6282705
@benjiprice88228 жыл бұрын
are you talking about restriction enzymes or is it something completely different? please do answer me, how about you @ozan ATASOY
@mariahfoley36457 жыл бұрын
What about RVDs
@dia69769 ай бұрын
talens by XANTHOMONAS BACTERIA
@ally8505 жыл бұрын
skip to 2:56, first few mins are bogus
@cannizzosam54175 жыл бұрын
* FOK1 not FOKL
@aronimitra286 жыл бұрын
RVDs in a particular TALE recognizes different bases. So change in RVDs results in change in sequence specificity. 9 TALEs don't copolymerize. Only two TALEs with different RVDs bind to two adjacent sides of Fok1 recognition sequence. You're explanation is pretty much wrong.
@nidhyajoseph14576 жыл бұрын
Can you please elaborate? I did not get what exactly was stated wrong here.. Also how are both the TALEs and REN -eg. Fok 1 made so specific for a particular sequence? Both the TALES are different sequences right? Are there any chances of both these TAL effectors binding to each other..?
@aronimitra286 жыл бұрын
Nidhya Joseph TALES from Xanthomonas has a DNA binding domain and an activator domain which allows it to regulate the activity of certain genes in it's host. The DNA binding domain has 30 repeats of 33-35 amino acid residues. Out of these 35 residues, residue number 12 and 13 are variable in the repeats and thus they are called repeat variable diresidue. These two amino acids are responsible for recognition of a single nucleotide in DNA. Amino acids NI at RVDs recognizes adenine, NG recognizes thymine, HD recognizes cytosine and NN recognizes adenine or guanine. So if you know the sequence of your DNA then you can design TALENS that bind to your specific sequence by changing the amino acids at 12 and 13th position of each repeat. In this way the talen can bind to your sequence and the DNA cleavage domain of Fok1 fused to the DNA binding domain of TALE can cleave the DNA. Generally two TALENS have to be coexpressed in a cell, one that binds upstream of the cleavage site and one that binds downstream. When the two will bind, two Fok1 domains come in vicinity and cleave the DNA molecule. The cleaved DNA can now be joined by non-homologous end joining which is an error prone double stranded break repair process , leading to incorporation of mutations in your desired sequence of interest.
@benjiehutchison1777 жыл бұрын
It's not FOKL, it's FOK1 The bacteria is also called Xanthomonas, not what was described in the video
@eathaare53587 жыл бұрын
nine-tails hah
@metinhuneren21636 жыл бұрын
I cant focus on video because of your pronunciation