This video is about the Electrophorectic Mobility Shift Assay (EMSA) and was made for MCDB 427 (Molecular Biology) at the University of Michigan. Figure 5.36
Пікірлер: 40
@asmaakterbhuiyan_shanu3951Ай бұрын
Best EMSA video even in 2024
@hsianloonАй бұрын
Love the step by step explanation of the results and why certain bands appear and others dont. Thank you
@phresie5 жыл бұрын
Best EMSA video ever! You just pulled together things that I have been seeing in peer-review articles (like supershift) and didn't know how to explain. THANKS!!!
@KJKP4 жыл бұрын
Seconded.
@loveforbioinfo5 жыл бұрын
The best explanatory video ever!!!
@read58693 жыл бұрын
I will never be depressed if the professor starts explaining stuff like this
@ahlamsoliman31515 жыл бұрын
Awesome and concise explanation!
@4Getf00l2 жыл бұрын
This is clear and concise. I have just realized that I don't analyze my gels so well. There's so much a gel can tell you from the bands.
@XY-yg1ci3 ай бұрын
The best understanding of EMSA TKS!!
@deebee00173 жыл бұрын
Thanks. I find all the relevant content in your videos.
@ang57984 жыл бұрын
This is a fantastic video and I thank you a lot
@user-nl9rb4qb8t9 ай бұрын
Great job! Thanks!
@nathanwilliams97786 жыл бұрын
Super Clear! Thank you!
@eloisub9022 жыл бұрын
WOW, great video! thanks a lot
@Yankee4ever24 ай бұрын
God Bless you!!
@Adeptbe6 жыл бұрын
thanks for the great video and clear explanation
@toribenner66274 жыл бұрын
amazing, thank you so much!
@bushra127110 ай бұрын
perfect explaining thanks !
@jacquelinelabovitz46136 жыл бұрын
YOU ARE A GD SEND!!!! THANK YOU!!
@anupamadhikari30886 жыл бұрын
Thankzzz maam.... that was really awesome...
@thatsnotme32353 жыл бұрын
Amazing!
@akhilamp3743 жыл бұрын
Wonderful explanation
@RUBIKCUKEN7 ай бұрын
your english is amazing
@geniussaiyan36085 жыл бұрын
So good love it
@santircastillo5 жыл бұрын
Thank you!
@doty31862 жыл бұрын
This video was really amazing. I am interested in EMSA, and I want to know can supershift could quantify RNA-protein interactions? such as a quantitative measurement to know if additional protein that binds to the first protein could reduce or increase the interactions? Thank you very much...
@evlampiaparcharidou40045 жыл бұрын
Hello! Very nice video, thank you so much! Do you think that after emsa could we take out the bands (free DNA vs DNA+protein complex band), purify them (carefully bcs we have redioactive P) and sequence them in order to see at which exactly DNA seq the proteins are bounding? You think is feasible?
@mpampiskarampatakakis94763 жыл бұрын
nice video. good explanation
@sujanidarshika3752 Жыл бұрын
Thank u so.much
@PuristicRaver4 жыл бұрын
Woho...awesome. Thanks for that nix explanation! :)
@neenujoseph937424 күн бұрын
Thank you🫂
@sofiatitah50393 жыл бұрын
THANKS!!!!
@tahasilat73943 жыл бұрын
Such a great explanation. Thank you so much!
@aasthashrestha30605 жыл бұрын
For example, we have two scenarios 1) we have two protein (A and B) A would bind to DNA and B would bind to A protein and 2) both A and B protein bind to DNA. Can we differentiate between these two conditions? because on both these scenarios we will have supershifts, correct?
@lauraarce93065 жыл бұрын
Deletion/ Mutational analysis of the DNA sequence.