Extracting Plasmid DNA: How To Do a Miniprep

Рет қаралды 52,098

2 жыл бұрын

In this method video, Molly takes us into the lab to teach us how to purify plasmid DNA from a liquid culture of bacterial cells. When purifying plasmid DNA from a small volume (3 to 5 ml) of bacterial cells as we do in this video, this technique is called a miniprep.
View this video (and more like it) on LabXchange: www.labxchange.org/

Пікірлер: 65

@samsonkamau5048 9 ай бұрын

I am not used to commenting but this one deserves a thumb's up. excellent explanation with clear and precise illustrations

@user-zd7xv7wc2t 6 ай бұрын

This video is one of the best teaching for learning extraction plasmid.

@bullymag Жыл бұрын

This work deserves way more than that amount of view. Excellent work. Liked and subscribed!

@birdo54 Жыл бұрын

This is a FANTASTIC video! Thank you very much, this was extremely well done.

@LabXchange Жыл бұрын

Glad you enjoyed it!

@shirinbehnamsani2366 Жыл бұрын

The first time I understood what I was doing this whole time in the lab. Thanks for the amazing explanation!

@mateuspaiva5916 Жыл бұрын

The material in the video is amazing, and the scientist is also an amazing actress! Great job in here, keep up with your inspiring work.

@MichaelSmith-ij2ut 5 ай бұрын

Hours of lecture and explanation distilled in to 15 minutes.

@gapat8213 Ай бұрын

What an incredible science humans has developed. This is something to be grateful for everyday. Plasmid will have the potential to cure all diseases when the special interests will allow it.

@isaacco2876 Жыл бұрын

this is a great video! thanks for the info!

@KatayonZare-uq9cm Жыл бұрын

That was PERFECT thank you for your explanation❤

@harp6198 Жыл бұрын

Thank you very much! This was excellent!

@pingsart7402 6 ай бұрын

wonderful demo

@nurmuhammad4540 Жыл бұрын

Excellent, thank you so much!!

@UnKnownVlogger01 4 ай бұрын

Every student like me deserve an advisor like you Mam 😅 Very well explained

@supermunheeb Жыл бұрын

Gonna do this tomorrow this helps me a lot! Thanks.

@brilllance8903 8 ай бұрын

perfect video. clear narration. overall 10/10

@michaelokeke7783 4 ай бұрын

Amazing! I love every bit of this video; so refreshing!

@srobles1660 9 ай бұрын

EXCELLENT WORK!!!

@reconeix 9 ай бұрын

Excellent, detailed, and approachable presentation. You're a natural educator!!!

@btssketch937 Жыл бұрын

Thanku so much for this amazing video I really appreciate your efforts ❤❤

@haleemasadia5225 8 ай бұрын

Thanks a lot Mam for such well illustration! It helped me a lot as I'm a student of MS biochemistry

@christiankemakolam7028 6 ай бұрын

protocol simplified and made interesting. kudos

@WeDream18 Жыл бұрын

Amazing explanation...❤ It covers each and everything you need to know. loves the way she explain.

@sebastiantraxler5550 4 ай бұрын

Amazing video!

@natalia8414 Жыл бұрын

Great video!😊

@parisahouri 3 ай бұрын

The best! Thanks a lot 😊

@blacktear5388 2 ай бұрын

This was super helpful, thank you so much

@isabelasoares1168 Жыл бұрын

Thank you for this amazing video!!! This channel will help me a lot

@shubhamaryan2972 8 ай бұрын

great explanation...

@03500 5 ай бұрын

It was amazing, can’t thank you enough 🙏🏻🙏🏻

@mobinahsansiddiqui 22 күн бұрын

Excellent Explanation, concise, and informative. Thanks.

@hana-al 5 ай бұрын

It was excellent !! thank you ❤️

@aamnasajid6570 5 ай бұрын

the best video I ever saw

@rufinamenezes7712 5 ай бұрын

An amazing and fun way of explaining. Thank you very much. Please continue it so that many may benefit from your explanations

@DiemNguyen-gg8gy 9 ай бұрын

Thank you very much for the excellent video! It is very helpful!❤

@alsaqar1 Жыл бұрын

Best video in this subject 🎉🎉

@salmanashraf8790 Жыл бұрын

Super method of explaining lady (teacher)

@user-lp5qe1tv7d 4 ай бұрын

Loved it thanks😊

@UdhayaBalaKanna111 5 ай бұрын

Just amazing!

@maxmordente2218 Жыл бұрын

Thank you!

@Brume20 7 ай бұрын

Amazing video.

@ayeshakhan279 Жыл бұрын

Thank You!

@Omar-Khaairy Жыл бұрын

Thanks so much

@minahil21 8 ай бұрын

Very well explained mam

@md.1276 2 ай бұрын

Don't get me wrong but I was here for science but stayed till the end for her smile

@taylormarkel1094 8 ай бұрын

thank you so much!!!!!

@shreeramesh9902 6 ай бұрын

awesome

@kadircanvural6814 Жыл бұрын

awesome! thank a lot

@arianevitoriasilvestrehann5140 5 ай бұрын

I loved it!

@alhaeri1 4 ай бұрын

legend

@Ganesh_lamichhane Жыл бұрын

How beautiful your video like your smile. I liked your video and your smile. Keep smiling and make videos.

@christopherleubner6633 24 күн бұрын

Very informative. Highly recommended for molecular bio and genetics students. ❤ ill take my plasmids chilled served neat. 😂

@omkarmore7613 2 ай бұрын

Very amazing with a very pretty mam

@wednesdaysfrog8422 9 ай бұрын

Which kit have you used?

@emmanuellaamara1876 15 күн бұрын

what kit did you use, I have been trying plasmid extraction for months now, but I haven't gotten a reasonable result

@ghazalehgolmohammadnezhadk5307 Ай бұрын

What happens if I forgot to wait for 1 minute before the last centrifuge?☹️

@rogersundeen4022 9 ай бұрын

Everybody got this? It's going to be on the test.

@lauraschonborn5633 Ай бұрын

You are so sweet and cool 🎉

@richardlange7559 6 ай бұрын

Some of that equipment is filthy!

@bebolovesshrek 17 күн бұрын

How

@badangel842 Жыл бұрын

I little bit confuse for Explanation O_o

@juxyclips671 9 ай бұрын

Whats her @?

@ThatStickyNote Жыл бұрын

Please do not inoculate your bacteria by poking a pipet tip by hand. Hold the sterile pipet tip with sterile tweezers (hold the tweezers in a blue flame for a sec) and then poke the colony on the agar. Also work under a flame or in a lateral flow cabinet.

@LabXchange Жыл бұрын

Thanks for the feedback! In this video, Molly is working with media that includes selective antibiotic. If she were working without antibiotics, she would have used sterile tweezers. It's important to note that best practices can vary, so you should always check with your PI if you have questions.