Flow Cytometry Protocol for Staining Membrane Associated Proteins

  Рет қаралды 31,084

Bio-Techne

Bio-Techne

4 жыл бұрын

This is a step-by-step video protocol on how to perform flow cytometry using Bio-Techne's conjugated antibodies to stain membrane-associated proteins in suspended cells. From this video, you will be guided through: cell preparation, experimental setup, FC receptor blocking, conjugated antibody addition and incubation, wash steps, and cell resuspension.
View full written version of this protocol: www.rndsystems.com/resources/...
Other flow cytometry staining protocols: www.rndsystems.com/resources/...
Flow cytometry workflow solutions:
www.rndsystems.com/products/f...
Need conjugated antibodies?
R&D Systems: www.rndsystems.com/search?key...
Novus Biologicals: www.novusbio.com/product-type...

Пікірлер: 3
@mamostamuhamad7326
@mamostamuhamad7326 3 жыл бұрын
thanks for this video
@MrBepis-qx5ye
@MrBepis-qx5ye 3 жыл бұрын
What is the purpose of phosphate buffered saline?
@user-sm5ww2ne9x
@user-sm5ww2ne9x 3 жыл бұрын
it mimics physiologic pH and ion concentration
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