FlowJo [UMAP]
Рет қаралды 3,755
Жыл бұрынAre you new to data analysis in FlowJo or looking for a starting point? This video will give you a quick overview on how to run a UMAP in FlowJo!
Looking for more FlowJo content? Let us know in the comments what other FlowJo content you would like covered!
@wendy40312 8 ай бұрын
Thank you for the wonderful tutorial, may I ask why are we excluding CD45 for UMAP generation? I understand L/D but don't know why we are not taking CD45.
@ajarieger_flow 8 ай бұрын
Thanks for asking! I didn't include it as it wasn't important to the biology I was looking at. This is something you should test with your samples- if including or excluding gives you better clustering.
@Ching-WenLin 5 ай бұрын
Hi Aja, thank you for sharing this. Is there a way to do it on flowjo, so that when we concatenated a group of patients based on their conditions, and we can see highlight on CD4 (example) and showing data per patient on the same UMAP? Thank you!
@ajarieger_flow 5 ай бұрын
There is! Before concatenation, give each group a keyword (usually 1, 2, 3, etc). Afterwards you can plot that keyword (use a linear scale) and gate them. Keyword 1 (group 1) will be at 1000, etc. You can then use these gates as you would any other gate. Hope that helps!
@Ching-WenLin 5 ай бұрын
@@ajarieger_flow Thank you so much for your reply. I did assign a keyword using numerical numbers, and gate them using a linear scale. But I am unsure how to show that on the UMAP or diagram, may I ask if you would mind sharing more information about this? Thank you!
@Jimo12m 5 ай бұрын
Hello, I working with cellls derived from different patients and we are characterizing all by some membrane marker expression. We wanted to create a UMAP based on the exprresion of different markers, i would like to know if we can do that, thank you!
@ajarieger_flow 5 ай бұрын
Hi! Thanks for reaching out. The expression of your markers is what the UMAP is using- you can select the markers to include or exclude when it set it up. Not sure if that answers your question- let me know if it doesn’t!
@RehabNady-rz7np Жыл бұрын
How to run Cytonorm, please.
@ajarieger_flow Жыл бұрын
Stay tuned! I’ll try get that out this week
@ajarieger_flow Жыл бұрын
CytoNorm video out today!
BalcevMMA_BOXING
Рет қаралды 20 МЛН
DeepFindr
Рет қаралды 1,8 М.
UC Merced SCIF Stem Cell Instrumentation Foundry
Рет қаралды 32 М.
Sameer Gaming
Рет қаралды 9 МЛН
lev89k
Рет қаралды 345 М.