Gas chromatography | GC

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Quick Biochemistry Basics

Quick Biochemistry Basics

5 жыл бұрын

Gas chromatography is a chromatographic technique used for the separation of
volatile compounds. The volatile compounds are the compounds that easily gets
vaporised at room temperature. Separation occurs based on the interaction of molecules with the mobile phase and the stationary phase. The less volatile molecules interact more with the stationary phase and moves slowly
where as more volatile molecules interact more with the mobile phase and moves fast. When the sample is detected the computer gives a peak with respect to the retention time of the sample.
The area under the peak gives information about the concentration of the sample.

Пікірлер: 182
@prangyadas5127
@prangyadas5127 Жыл бұрын
A detailed information in a very shorter period of time and was explained in a crystal clear manner. Excellent.
@sayurirathnayake48
@sayurirathnayake48 4 жыл бұрын
Thanks a very lot.i studied A to Z clearly from this video.it's amazing how you explain them simply.please make more & more videos.Good Luck
@prayoga4419
@prayoga4419 3 жыл бұрын
Very brief and to the point. Love it. Thank you so much.
@pepe6666
@pepe6666 2 жыл бұрын
oh dude thank you. finally a way to understand this. the terminology of a gas chromatograph is used so much with mass spectropy that its difficult to figure out whats what. i had always thought those peaks on the chart were speaks on a spectrum! this has cleared a lot up for me, thanks.
@tuffel03
@tuffel03 2 жыл бұрын
Thank you this was the exact format and presentation I needed
@sarahkamau421
@sarahkamau421 4 жыл бұрын
Very well done! You're really helping us beginners! Thank you!
@trevorndhlovu7834
@trevorndhlovu7834 4 жыл бұрын
COOLSOME data.It's awesome staff you arranged here.
@mustafeabdiazizismail3771
@mustafeabdiazizismail3771 2 жыл бұрын
Thanks 🙏 to your efforts, we finally understand the different types of chromatography
@sydneym3251
@sydneym3251 2 жыл бұрын
great video, great explanations, and i love your drawings/ animations!
@henrycalhoun1119
@henrycalhoun1119 4 жыл бұрын
thanks dude, very clear and helpful!
@sarahholland5980
@sarahholland5980 3 жыл бұрын
this is amazing. so simple and clear. thank you!!!!
@Boooommerang
@Boooommerang 4 жыл бұрын
Excellent video! Thanks
@aga4966
@aga4966 3 жыл бұрын
Really good video, made me understand GC!
@table2790
@table2790 3 жыл бұрын
You are absolutely amazing! Thank you So much :))
@softvibes4811
@softvibes4811 Жыл бұрын
This video totally cleared everything up, thank you so much
@rimm2592
@rimm2592 Жыл бұрын
I like these videos. They just make my life easier. Thank you so much for the explanation!!!!!!
@Carmelo12320
@Carmelo12320 2 жыл бұрын
Thank you exactly what I needed.
@fantasticcreativity6136
@fantasticcreativity6136 Жыл бұрын
O my God,,,such an amazing explanation Love it,,, clear my all doubts and confusion
@temmietemmieson6756
@temmietemmieson6756 2 жыл бұрын
Really well explained thank you!
@chandrashekarn5376
@chandrashekarn5376 4 жыл бұрын
Good explanation sir with animations 🙏🙏
@triciajones6923
@triciajones6923 2 жыл бұрын
So very good.Thank you!
@AntonioAdame1
@AntonioAdame1 4 жыл бұрын
Great vid. Thank you
@hysysplusunisimandchemcad4499
@hysysplusunisimandchemcad4499 2 жыл бұрын
Excellent Work, Thumbs Up.
@palanirajramalingam1131
@palanirajramalingam1131 4 жыл бұрын
WELL EXPLANATION EASILY UNDER STAND THANK YOU
@divya.t.george6030
@divya.t.george6030 3 жыл бұрын
very well explained understood it greatly
@JERKINSRICE
@JERKINSRICE 4 ай бұрын
Amazing! Very well explained. Thank you very much.
@sibghatkhan3986
@sibghatkhan3986 5 ай бұрын
Stupendous!! It is great effort You have make it clear as crystal 🔮 mirror
@shynosalexander9200
@shynosalexander9200 3 жыл бұрын
This was very helpful. Thank you :)
@wen6519
@wen6519 3 жыл бұрын
That was helpful, thank you for sharing
@themoreyouknow585
@themoreyouknow585 4 жыл бұрын
Amazing I really appreciate it
@jono2167
@jono2167 3 жыл бұрын
Excellent video. Very clear and informative. Good job mate!
@mdraselmiah3403
@mdraselmiah3403 4 жыл бұрын
good explanation and it will help me more..thanks
@Ara2061
@Ara2061 4 жыл бұрын
thank you.. .thank you... thank you
@mustakim2144
@mustakim2144 3 жыл бұрын
Thank you!
@hajrabarool6044
@hajrabarool6044 3 жыл бұрын
This video made my day today
@stellalesirite4059
@stellalesirite4059 3 жыл бұрын
Thank you for this🙏
@satyasurya8923
@satyasurya8923 3 жыл бұрын
Thank uuu sooo much for this wonderful explanation 👏👏👏
@arbazchaudhary5752
@arbazchaudhary5752 Жыл бұрын
thanks for this video you explained very well
@bankthanadorn4598
@bankthanadorn4598 3 жыл бұрын
Well explained, better than my lecturer tbh thankss
@abrarrakin1757
@abrarrakin1757 4 жыл бұрын
superb man, thanks a lot
@faizakainat9427
@faizakainat9427 3 жыл бұрын
impressive..beautifully explained
@mariofelipequevedolozano3659
@mariofelipequevedolozano3659 3 жыл бұрын
Thanks a lot!!!
@rabiaameer8588
@rabiaameer8588 2 жыл бұрын
Amazing lecture clear my all ambiguous points about GC
@esterindahkusumaputr
@esterindahkusumaputr 9 ай бұрын
Thank you for your video, it's clear
@dimitrikolotauri6622
@dimitrikolotauri6622 4 жыл бұрын
thank you sir.!
@nargesmaqsudi62
@nargesmaqsudi62 4 жыл бұрын
Thanks a lot
@ianedmonds9191
@ianedmonds9191 3 жыл бұрын
Excellent. Thanks for that. Luv and Peace.
@carmempacificodasilva4799
@carmempacificodasilva4799 Жыл бұрын
thank you so much!!!
@_sri_702
@_sri_702 2 жыл бұрын
Thank you 👍
@MrKpy86
@MrKpy86 2 жыл бұрын
I work for a company in the Chemistry industry that manufactures instrument and that was pretty accurate, good job. Only thing is inlet is at 20-50C higher than the max temperature reached by the GC and there is also an inlet of Makeup Gas for the FID which is usually to compensate the fact that nitrogen or helium coming from the column is usually much lower than the other gases. Other than that, great video
@Rootfresh_channel
@Rootfresh_channel 3 жыл бұрын
Last sem exams I prepared some of difficult topics from your channel and got a 86/87 marks..
@thatvid.6628
@thatvid.6628 3 жыл бұрын
WOW! I am still repeating.
@daredevil6117
@daredevil6117 2 жыл бұрын
Wow online me itne marks.😜
@sabahedayati4474
@sabahedayati4474 Ай бұрын
Thank you🌱💙
@imtme
@imtme 2 жыл бұрын
thank u, u are so helpful, more helpful than my lecturer
@user-yt2yl3fn4z
@user-yt2yl3fn4z 9 ай бұрын
Respect 🙏 from Africa Zambia better than my lecturer for a year thanks I know love this course❤
@ValeriaGobjila
@ValeriaGobjila Жыл бұрын
thank you for this informational video on behalf of all science students :)
@halimzaini1796
@halimzaini1796 4 жыл бұрын
Thank you
@jayliu7786
@jayliu7786 2 жыл бұрын
i have a organic chemistry lab tonight and hoping that it goes well. thanks for the video, it's very insightful :)
@sujikumar7831
@sujikumar7831 4 жыл бұрын
Super..very useful.. simple
@kamnabharati2970
@kamnabharati2970 2 жыл бұрын
Thank you Sir.❤️🙏🏻
@shourovhossain8498
@shourovhossain8498 2 жыл бұрын
Amazing.....the video clear the concept of chromatography.
@dinosanchez8528
@dinosanchez8528 3 жыл бұрын
Good video, thanks
@zahidhussainzaigham9223
@zahidhussainzaigham9223 Жыл бұрын
Well explained briefly. Thanks
@BrainKhan
@BrainKhan 3 жыл бұрын
Nice one explanation i always found there
@baochau4877
@baochau4877 Жыл бұрын
thsnk you so much, very helpful
@user-wt9gv3kd3q
@user-wt9gv3kd3q 7 ай бұрын
Thank you!!!!!
@bijiteshchoudhury8274
@bijiteshchoudhury8274 2 жыл бұрын
Well explained Thank you
@9madhumitha928
@9madhumitha928 2 жыл бұрын
Very useful ☺️👍 thank you
@juliana_jesus
@juliana_jesus 2 жыл бұрын
Thank you very much!! May I have the references for further reading please?
@yasminarijah1646
@yasminarijah1646 Жыл бұрын
Thank you. Very helpful
@jignasachiramana8269
@jignasachiramana8269 2 жыл бұрын
really helpful !
@hisanathasni5499
@hisanathasni5499 3 жыл бұрын
Thanks bro ❤
@seeker8906
@seeker8906 4 жыл бұрын
Thank you Sir
@sequeira7330
@sequeira7330 7 ай бұрын
summary of the video: GC is used for the separation of volatile compounds( compounds which are easily vapourized at room temp) Components of GC: 1) SAMPLE is mixed withe a highly volatile compound like Hexane, cyclohexane, etc. • Long coiled column: can either be a packed column(made of glass/stainless steel) or Capillary column (made of fused quartz). this column is placed in a CHAMBER in order to facilitate a uniform temperature. (HIGH TEMP) During analysis, placed at 150-300°C • the SP is packed in the column. it is usually made of silicon grease or wax as it can withstand high temperature • Presence of a SEPTUM to inject sample just before the chamber - the temperature of the injection region is 20-50° higher than chamber. this is to facilitate the rapid vapourization of the molecules. 2) Mp: usually helium / nitrogen (inert/unreactive gases are used) are placed in a cylinder connected to the Column via the Molecular sieve. 3) Molecular sieve is used to filter out unwanted HCs, Water vapour, oxygen etc that may interfere with the sample during experimentation. 4) Detector
@sequeira7330
@sequeira7330 7 ай бұрын
Detector - common: FID ( FLAME IONISATION DETECTOR ) which has 3 inlets: • carrier gas from the column • hydrogen • oxygen ignitor ignited the H2 and O2 to produce a flame. when sample molecules reach the flame, they get ionized and electrons are released. across the flame, there are 2 electrodes ( cathode for cations, and anode for anions) the electrons generated are detected by the electrodes in the form of current and it is amplified by the amplifier/Computer.
@sequeira7330
@sequeira7330 7 ай бұрын
the computer then generates a graph: X axis- time y axis- current based on known samples and comparing their output with the output of the unknown samples, the retention time/concentration can be deduced
@nh-vt5ws
@nh-vt5ws 6 ай бұрын
​@@sequeira7330 seems like you're a technician of GLC... Excellent explaination bruh🎉
@user-dq9ty5tq1z
@user-dq9ty5tq1z 2 жыл бұрын
thank you sir for your good explaination.
@bhaveshthakor4219
@bhaveshthakor4219 7 ай бұрын
Amazing explanation
@mumizashahid6047
@mumizashahid6047 2 жыл бұрын
Very very very very helpful Thanks alot for this
@muhammadammar6262
@muhammadammar6262 3 жыл бұрын
very well done video very good keep it brother
@praveenkumarkp4084
@praveenkumarkp4084 3 жыл бұрын
Very helpful 👍 plz do classes of HPLC and spectrophotometer
@nihalanasrin9598
@nihalanasrin9598 4 жыл бұрын
Highly useful
@muskanagarwal1173
@muskanagarwal1173 3 жыл бұрын
Amazing sirr
@ankitagaonkar4298
@ankitagaonkar4298 4 жыл бұрын
Wow explained well
@aminechahid2004
@aminechahid2004 4 жыл бұрын
THANK YOU
@j.d.tgaming7199
@j.d.tgaming7199 3 жыл бұрын
Hiee
@user-kt3xu3ml1q
@user-kt3xu3ml1q 2 жыл бұрын
It's really helpful ...thanks 💜
@GuruDestiny
@GuruDestiny 3 жыл бұрын
Thanks😍
@Matteo-mx1sz
@Matteo-mx1sz 3 жыл бұрын
thanks
@mairaseiilgazy7510
@mairaseiilgazy7510 3 жыл бұрын
Thanks
@pharmasolutionpakistan7668
@pharmasolutionpakistan7668 2 жыл бұрын
Good explaination
@EnglishExcel2208
@EnglishExcel2208 4 жыл бұрын
Super xplanation
@amitgupta-yr5pl
@amitgupta-yr5pl Жыл бұрын
Great present
@user-jd3bk2wq6m
@user-jd3bk2wq6m 2 жыл бұрын
In bad need for applications of gas chromatography 🥺💔
@I_sha_rma_
@I_sha_rma_ Жыл бұрын
Amazing 👍❤️
@laylachemist5245
@laylachemist5245 4 жыл бұрын
Well done sir
@b4baigan989
@b4baigan989 11 ай бұрын
Wow... Just wow
@alaulislam1869
@alaulislam1869 Жыл бұрын
Brilliant explanation dear sir☺️❤️
@junaid_ahmed1155
@junaid_ahmed1155 Жыл бұрын
Very useful
@seripahnyambura2782
@seripahnyambura2782 3 жыл бұрын
Thankyou
@khushbujha4957
@khushbujha4957 4 жыл бұрын
As we can say in 6min our separate sample is there??
@spurthiblk3306
@spurthiblk3306 4 жыл бұрын
tq ...Vry useful
@venkatk669
@venkatk669 4 ай бұрын
Good information
@mohammedyunus4115
@mohammedyunus4115 2 жыл бұрын
Great
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