ELISA (Enzyme-linked Immunosorbent Assay)

Рет қаралды 325,604

Күн бұрын

Hey Friends,
ELISA, short for 'Enzyme-linked Immunosorbent Assay', is a powerful technique to detect substrates (e.g. an antigen). There are multiple subtypes and variants of ELISA. Here, the most common Sandwich ELISA is explained.
More about the DIRECT ELISA:
• Direct ELISA in 1 minute
More about the INDIRECT ELISA:
• Indirect ELISA in 1 mi...
An article about the Enzyme-linked Immunosorbent Assay:
www.thermofisher.com/de/de/ho...
Cheers
Henrik
Time Code:
0:00 What is an ELISA?
2:00 Sandwich ELISA example

Пікірлер: 60

@henrikslab Жыл бұрын

From now on you can become a member of this channel! You will have access to some cool emojis like this:. This is of course voluntary, but the financial support helps me a lot.

@wodecka736 2 жыл бұрын

dude, you're saving my life for tomorrow's biochem module

@dibyajyotichattopadhyay7154 3 ай бұрын

Seriously

@StevenMP1993 10 ай бұрын

The one video on ELISA that actually helped me understand. Thanks man!

@sarcicek6864 9 ай бұрын

I've discovered your channel yet. It is amazing for molecular biology students. 🙏🎉

@artfromtheheart833 Жыл бұрын

Precise concise and accurate thanks alot

@mayaskaaa 10 ай бұрын

so easily explained. thank you so much ❤

@MAZINO225 Жыл бұрын

Today is my biochem University. Thank you

@chang6362 2 жыл бұрын

Very helpful video. Thank you!

@rebeckaeriksson3731 3 жыл бұрын

This is a great video! Thank you!

@mahmad3825 2 жыл бұрын

Thankyou very much. You made it so simple

@yelxi 2 жыл бұрын

Thank You, Sir. You made it so easy to understand.

@priya24612 Ай бұрын

thia channel deserves more than the views it already has ❤🎉

@yeukaiwadzanaingwazane3680 2 жыл бұрын

Thank you very much.. your videos are helpful

@axediepied6993 2 жыл бұрын

Amazing explanation

@isabelizseijo2781 2 жыл бұрын

super easy to understand thanks

@timb7298 Жыл бұрын

thank you for this insight

@naveenkamala9472 2 ай бұрын

Great presentation

@SaahilMohta 2 ай бұрын

Great explanation!

@Isa-gy5yg 3 жыл бұрын

Thank you!

@guerroujadam8812 Жыл бұрын

man u made it so easy to understand even for a Frenchman ty so much ur video is easier to understand than my lecon xd

@maisamelsayed 11 ай бұрын

Thank you a lot! 😁

@Us33rr6 2 жыл бұрын

Vielen dank!!

@masstorm7724 Ай бұрын

Danke Henrick :)

@tunetonic04 2 ай бұрын

Thank you so much

@mahxylim7983 Жыл бұрын

thanks! i got it in a glance

@user-rh1dy5if9q 4 ай бұрын

Thank you❤

@sanjaykumar-ew6mb 6 ай бұрын

❤ thanks for the nice video dear sir ❤

@strivingforexcellency Жыл бұрын

Thank you

@notme7310 Жыл бұрын

Omg thank you so much!

@albertovisconti352 Жыл бұрын

Hi, what programme do you use to make these animations?

@AnjorinOmotola 7 ай бұрын

Bless you ❤

@09gabriel92 2 ай бұрын

We love you man

@gilian2587 3 жыл бұрын

Beer's law -- applied to proteins; that's neat. But it looks like the assessment to determine the quantity of protein in a sample is strongly dependent on historical data; where you can compare similar samples to each other. ... ... or just read the data sheet provided by the supplier of the ELISA. After doing some reading on this... it looks like most ELISA samples are actually created by specialized laboratories that hire a lot of Peptide Chemists. Surprisingly... Peptide Chemists are paid around $45,000-75,000 per year (well; for ONE datapoint, but that ONE datapoint was requiring that the applicant had a PhD). I am a bit surprised at how saturated the Chemistry job market really is at the moment; that seems like low pay for pretty highly skilled work.

@Raphaelpoh Ай бұрын

Which type of ELISA is suitable for very small viruses such as mouse parvovirus?

@reet1825 11 ай бұрын

Thnk u ❤❤

@ornulusoundeffects6423 17 күн бұрын

Is this the same thing what we do in SDS page?

@izza4780 Жыл бұрын

Nii shoooperadaa 🙈🙈

@zoedk2266 3 жыл бұрын

Thankyou!

@fontexstudios 2 жыл бұрын

what illustration program do you use?

@henrikslab 2 жыл бұрын

Microsoft Office Powerpoint

@user-pt5iv6um6r Жыл бұрын

Tôi thích sản phẩm này

@DestinyCorton Жыл бұрын

is it qualitative or quantitative?

@markdolan914 6 ай бұрын

Good info.. from Bruno😏🤣💀 #yahhh

@pacokolb885 Жыл бұрын

So whats the diffrence between western blot and elisa indirect variation?

@henrikslab Жыл бұрын

I assume you have noticed that both techniques use an antibody against the antigen and another secondary antibody which is detectable! The difference is that western blot (WB) is able to reveal the protein size, whereas ELISA is not. Also, ELISA allows for a more accurate quantification that WB.

@pacokolb885 Жыл бұрын

@@henrikslab thank you very much:)

@arunmoses2197 Жыл бұрын

@@henrikslab How does ELISA allow for more accurate quantification?