I do professional microscope service. Although it was very nice to show people all the little pieces in a typical objective I found myself saying, "not that way !" to the computer screen several times ! 1. the back piece which works as an aperture, SCREWS off, it doesn't pry off ! Usually, quite easily by just pushing it against a nice rubber surface and rotating. 2. The sets of 3 triangulating holes on the barrel are for the factory to center the lens elements. 3. DON'T use your calipers as a wrench. Aarrgg ! You will just bend up your calipers and potentially slip and damage the lenses. They do make nice lens tools for that job. Otherwise, enjoy your informative videos. Most frequent reason to take apart an objective is to remove oil. Painstaking process to get the elements really perfectly clean afterwards. Sometimes can not be repaired due to damaged elements or a leaking seal at the front element. Probably about an 85 to 90 percent success rate. Often have to "play" with centering the elements several times to get the optimum image, so can also be very time consuming. However, If one is looking at several thousands of dollars to replace an objective it is often worth and time and expense of trying to repair it first !! I enjoy your videos, keep educating people about microscopy, there is always more to learn !!

They used to make different thickness spacers to use to adjust for parfocality. A quick way I will often use is to put a little latex caulk on the objective threads. I'll adjust the lens by screwing in to match the focus and then leave it to dry. It still easily unscrew for removal when necessary.

Good video. I would like to add that the plastic cover at the top of the objective can be easily screwed off instead of popping it out with a screwdriver. I do it a lot. Thanks for your informative videos

thanks for sharing...I was thinking about opening my objective out of curiosity...

I think the multiple screw holes is so you can choose on that better suite you macroscope in making all objectives parfocal.

A calliper! Great idea. I can’t get mine off

Good knowledge gain about objective lens

Thank you for the very detailed video 💕✅

Thanks, it answered for my question: did I made a bad decision for unscrewing that little screw and rotating the internal part too much, that I even don't remember the last position of the rotation, where the screw was screwed :D I was afraid that it was some kind of focusing regulation, but now I understand that it's just for the stability to prevent rotation.

Nice. I have 2 videos here on youtube where I am dissasembling Mitutoyo 10x NA0.28 and 20x NA0.42 objectives.

My random thought early this morning: I wonder what a microscope lens looks like disassembled.... Thanks!

look at all the parts at 40x

Newbe question: When changing objectives for each magnification level, I need to fine focus about a quarter turn. Going from X4 to X10 though, it takes a full turn. If I unscrew the X4 objective about 2 turns and use a gap gauge (0.3mm) I find that I only need to adjust a quarter turn like the others. Do they make shim washers to correct this?

Anyone buying the excellent and still expensive objectives from the early 1900s that can be found on eBay would be advised that they used to employ "drunken threads" so that the elements could be aligned precisely on an optical bench with special equipment. It would be impossible to reassemble the objective without that equipment. Not a problem with the modern objectives here.

Gracias por tu trabajo

How did you break so many objective lens? Share your experience please, so that we don’t have to.

sir I'm planning to build a Galilean telescopes. now my question is . will it work as my eyepiece lens?

Microscope repairing shop were having

Respected Sir, First of all thanking you for preparing such nice and knowledgeable video. I'm a research scholar and having my own microscope and related camera of Dewinter company and facing lots of fungal problems in all my lenses and also in the camera. I regularly cleaned it from lens cleaner and then all lenses were washed with absolute alcohol but with in two days this problem again occurred. One of my camera adaptor lens is damaged due to this cleaning processes and 1000x photographs shows lots of scratches in the photographs and poses lots of pressure in my mind because 1000x photographs are necessary for my research paper. So please suggest me how to protect lenses from the fungus. Thanking You.

So can i clean the inside of the one with the small hole with alcohol or should i also use dish soap?

Can i take it apart and use ultrasound clean clear them?

How to buy lense in amazon

I have a question does microscope lenses convex?

sir and you make lap microscope into mobile repair microscope it will be hip t

Sir I hope to make a microscope from scratch. please help me to do it. I hope your reply

Xds