How electrospray ionization works

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Күн бұрын

What is electrospray ionization?
How does ESI work?
How to stabilize an electrospray?
ESI stands for Electro-Spray Ionization.
Ionization efficiency and ionization stability are important in mass spectrometry because the quality of the data depends ion the quality of the spray.
Electrospray and nano-electrospray are used for proteomics and metabolomics. Scientists and analytical chemists invest a lot of their time to get stable sprays.
I made and filmed an electrospray to show what is does when is stable, and what happens when it is unstable. This video is a hands-on introduction to electrospray ionization.
Professional electrospray and nano-electrospray solutions:
www.fossiliont...
The Sharp Singularity nano-electrospray emitters:
www.fossiliont...

Пікірлер: 58

@wordsbyruthie 2 жыл бұрын

I really enjoyed that u were having fun while explaining this. Good job.

@playingwithions 2 жыл бұрын

Thanks a lot! I'm glad you enjoyed :)

@gizmogizmo5959 2 жыл бұрын

This is the best ESO expansion i heard so far, I hope we will see more videos in the near future. Thanks a lot!

@playingwithions Жыл бұрын

Thanks a lot gizmo gizmo. I plan to make more videos, just have to find the time 😅

@ninjadog5800 2 жыл бұрын

Thank you so much there's not a lot of good videos on electrospray ionisation I hope it covers everything on my test

@playingwithions 2 жыл бұрын

Thanks! I wish you the best for your exam :)

@SanDiprtv07 10 ай бұрын

Great representation,it made easy to understand 👍👍

@anwaralmajed6177 Жыл бұрын

fun way to learn thank you for making it interesting

@playingwithions Жыл бұрын

Thanks Anwar for the feedback. I would like to make more videos. Just have to find the time 😅

@massspec8718 2 жыл бұрын

This is truly great work.

@playingwithions Жыл бұрын

Thanks MassSpec,

@alexb5391 2 жыл бұрын

What an interesting video. Thank you for this content, and hope to see more from you.

@playingwithions Жыл бұрын

Thanks Alex B for the positive note. I plan to come back to making more videos.

@ronbessler1271 3 жыл бұрын

Wonderful Video! Thank you so much for this!

@playingwithions 3 жыл бұрын

Thanks a lot to you, Ron! I think youtube is a great place to post educational content. Let's see how the channel goes :)

@user-ys1rz1tx3e Жыл бұрын

SO nice video. thanks

@playingwithions Жыл бұрын

Thanks :)

@javydemiguel7351 3 жыл бұрын

Thanks for the video!! Great knowledge and very helpful!!

@playingwithions 3 жыл бұрын

That is the Idea Javy!

@edgarestrellaparra2945 2 жыл бұрын

Hi interesting video i work as spectrometer in hplc, particularly metabolomic. I interesting in bioinformátic to metabolomic. Thanks again

@_han_da__8216 4 ай бұрын

a really good explanation! thank you so much! :))))

@aggunnamadhumita2281 2 жыл бұрын

fantastic video....

@playingwithions 2 жыл бұрын

Thanks for your comment. Glad you liked it :)

@segunkogbodoku4324 Жыл бұрын

Great explanation

@FreedomAddicted Жыл бұрын

Yeah, now I know how the world works. Thank you for the vid.

@chosy-ut9xl 4 ай бұрын

I want to know about the negative electrospray ionization. Why corona discharge occurs in detail !!

@fabzy4L 2 жыл бұрын

Buenísimo, haha y la referencia al unicornio en microfluidos

@kubasmetana5525 8 ай бұрын

Great video, I like your model. How are you apllying the voltage on the "sample" when is your capilary made from plastic? Thank you.

@playingwithions 8 ай бұрын

Hi kubasmetana5525! The voltaje was applyed by inserting a metalic pin in the tube. The voltage is applied to the pin, which is in contact with the liquid.

@elvirademiguel7357 3 жыл бұрын

¡ Muy interesante!

@alikocadere254 3 жыл бұрын

Teşekkürler

@poojakshetri5801 2 жыл бұрын

Amazingggggg..... Interestinggggg.....

@playingwithions 2 жыл бұрын

Thanks for commentinggggg... 😉

@Freepalestine4.0 2 жыл бұрын

Can you recommend the whole setup (machine) for electro spraying. Which company is better with better efficiency. Name of company and model number of device.

@playingwithions 2 жыл бұрын

Hi Trending, Thanks for your comment! The ionization efficiency in nano-electrospray depends on the emitter. The ion source provides positioning, voltage, connection to the MS, and so on. In short, the ion source of your MS should work fine. If you are interested in performance, choose the best emitters. Fossiliontech sells and ships worldwide. You can check the nano-esi emitters here: www.fossiliontech.com/nanoesi-emitters

@srirampmna135 4 ай бұрын

Could u pls explain what happens when vinegar is added

@srirampmna135 11 ай бұрын

The video is really interesting and easy to understand . Could u please explain why ESI is best suitable for analysis of polar compounds

@playingwithions 11 ай бұрын

Good idea for a future video. I'll try to resume the channel soon :)

@srirampmna135 11 ай бұрын

@@playingwithions Hi.I am a purification scientist .I find ur videos really amazing.wouod be happy if u continue doing videos on these topics

@starfish8D Жыл бұрын

fun. good job.

@meganpass8011 2 жыл бұрын

For chain ejection long molecules are rejected due to what?

@playingwithions 2 жыл бұрын

In chain ejection, long molecules are ejected due to Coulomb repulsion. A key difference between chain ejection and ion evaporation is that the energy required to eject each link is lower than the energy required to eject the entire molecule at once. I hope this helps! :)

@yueyangli5472 Жыл бұрын

Nice video! I noticed that sometimes the mass spec in our lab doesn't generate ionized droplets, in other words, the liquid can not be emitted into the orifice. We apply 3 kV but the signal is always not stable. Do you have any ideas?

@playingwithions Жыл бұрын

Hi! There are several factors affecting the stability of the spray. Have a look at these videos: kzfaq.info/get/bejne/gsuZZaxmzM-2iKs.html kzfaq.info/get/bejne/e5iIecun1M7Fh2Q.html