I can't even focus on what he's saying because all I hear are those wet mouth noises that melt my brain
@User_abUser394 жыл бұрын
Jesus Christ 🙏😂
@husseinkaraki69194 жыл бұрын
😂😂😂😂😂
@TCioffi3334 жыл бұрын
Makes it unwatchable, unfortunately
@charliejin86204 жыл бұрын
Every protein man deserves such an ultimate perfect instruction.
@abolfazlnik-akhtar16672 жыл бұрын
A comprehensive overview of Mass spectrometry. Good job Matthew.
@Rueyful3 жыл бұрын
This is absolutely AMAZING. Thank you and please make more videos science needs you.
@joa20235 жыл бұрын
Took me a while to find this explanation. Perfect overview of the subject.
@AlternativeAdventureAthlete6 жыл бұрын
Very well done! great amount of info for under 30 min.
@MelodySaleh4 жыл бұрын
Thank you! You are so good at explaining honestly.
@woillusion1236 жыл бұрын
Very great explanation! Thanks- now I get an idea of how mess spectrometry works for biomolecules.
@editingone85054 жыл бұрын
Great video! I learned a lot, thank you for the hard work.
@tomgrev6 жыл бұрын
Thanks Matthew, this great material!!
@francescosbarbati02 жыл бұрын
Best explanation I saw. Congrats!
@sbcand70775 жыл бұрын
fukken brilliant m8. thanks a lot for the detailed intro.
@lorenzo.t.674 Жыл бұрын
Bloody legend mate
@NORTON2000105 жыл бұрын
Thank you!!!!
@hostanes52 Жыл бұрын
Thank you so much, I definitely owe you one 👏🙌
@miloschaal281611 ай бұрын
Thank you so much! I needed this for my uni course.
@user-eg4gu6sl4p3 жыл бұрын
thanks so much
@15837pawimomo6 жыл бұрын
thank you for uploading
@timho39216 жыл бұрын
thank you so much!!!
@akhilratanmishra42133 жыл бұрын
elaborate explanation
@markopoloasia4 жыл бұрын
Cheers!
@kylekookie14404 жыл бұрын
Brilliant but good lord it sounds like you're presenting to the Top 30 CEOs of the Fortune 500. Get some water homie.
@MatthewPadula3 жыл бұрын
You don't like the sexy huskiness? ;)
@Factsraven3 жыл бұрын
This is a general theoretical on mass spectrometry. Would be more useful if top-down and bottom-up approaches in proteomics were covered.
@MatthewPadula3 жыл бұрын
Yes. It is on the to-do list. Hopefully in the next few weeks, these will start to be updated. They take a long time to get done properly.
@OsamaShehzad19955 жыл бұрын
Great video, but this guy needs to take some pauses in between!
@anjunagpal68465 жыл бұрын
How can one determine the molecular mass of protein by the peaks given in graph?
@MatthewPadula5 жыл бұрын
In ESI, with a single pure proteofrom being ionised, each peak will represent a different charge state of the protein, with a smaller m/z being a higher charge state (more H+ attached). To work out the mass of the protein, simultaneous equations can be used and this can be done manually with enough time. Software that comes with the MS often does this, but there is also online calculators like this one: www.bioprocess.org/esiprot/esiprot_form.php.
@temiloluwabolajioyenekan62104 жыл бұрын
Hello Dr. I will like to know if you have a published paper concerning this overview because I will like to use it as one of my references for a research paper I'm writing on "Translational Proteomics with Advanced Mass Spectrometry (MS) Systems".
@mattpadula8374 жыл бұрын
This is all out of my own head using articles and text books that were already out there, along with discussions with engineers. So I don't have a single article. If you email me, I'll try and find some srticles to send you.
@namunamu90278 ай бұрын
like and subscribed 👍
@sibelozdemir595 жыл бұрын
Great information, thank you, but kindly are you a smoker Dr?
@MatthewPadula5 жыл бұрын
Bwahahahahahahaha! No, I'm not a smoker. But the audio was recorded at about 7am in my office by myself with a headset. I realise that I need to be more dynamic.
@sibelozdemir595 жыл бұрын
@@MatthewPadula oh great, I just thought you had breathing difficulty, I would not even be speaking that early and again thank you for the videos. 😊
@medbolm42305 жыл бұрын
go from the simple thing, don't mix the stafs
@AGtheguitarist3 жыл бұрын
Myoglobin 11:35
@rodrigosanchespires37934 жыл бұрын
@16:46 What the heck is an "uncharged ion"????????
@MatthewPadula4 жыл бұрын
Four years and someone finally picks up a 'mistake'. You tell me what the common name for an uncharged ion is, one that is able to enter the MS, be past through the instrument and able to strike the detector, giving a false signal? An uncharged molecule, I guess.
@SethCrosby2 жыл бұрын
The Thermo video (kzfaq.info/get/bejne/gdCFhLym3pnRo5U.html) is no longer available.
@MatthewPadula2 жыл бұрын
Remind me where this is referred to in the video.
@SethCrosby2 жыл бұрын
@@MatthewPadula 20:17
@mattpadula8372 жыл бұрын
@@SethCrosby Thanks. I will fix it for the next version.
@Creator917 ай бұрын
HOW DID YOU IDENTIFIED THESE ARE ISOTOPES OF CARBON
@kellymcgrath55414 жыл бұрын
dada
@abdulazizabdunasimov89783 жыл бұрын
deeeeeeeyta you say, huh?
@neilmccracken1604 жыл бұрын
This is a great summary but there are some serious copyright infringement issues with this video. The simulations in the LC-MS are straight from Thermo Scientific. There is no credit attributed to Thermo anywhere in the video and it is not clear that permission has been given for use of their video.