Mass spectrometry for proteomics - part one

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Matt Padula

Matt Padula

7 жыл бұрын

Пікірлер: 55
@stevelau-rivera7188
@stevelau-rivera7188 5 жыл бұрын
Somebody please get this guy a glass water.
@martingby9089
@martingby9089 5 жыл бұрын
THANK YOU
@martingby9089
@martingby9089 5 жыл бұрын
I can't even focus on what he's saying because all I hear are those wet mouth noises that melt my brain
@User_abUser39
@User_abUser39 4 жыл бұрын
Jesus Christ 🙏😂
@husseinkaraki6919
@husseinkaraki6919 4 жыл бұрын
😂😂😂😂😂
@TCioffi333
@TCioffi333 4 жыл бұрын
Makes it unwatchable, unfortunately
@charliejin8620
@charliejin8620 4 жыл бұрын
Every protein man deserves such an ultimate perfect instruction.
@abolfazlnik-akhtar1667
@abolfazlnik-akhtar1667 2 жыл бұрын
A comprehensive overview of Mass spectrometry. Good job Matthew.
@Rueyful
@Rueyful 3 жыл бұрын
This is absolutely AMAZING. Thank you and please make more videos science needs you.
@joa2023
@joa2023 5 жыл бұрын
Took me a while to find this explanation. Perfect overview of the subject.
@AlternativeAdventureAthlete
@AlternativeAdventureAthlete 6 жыл бұрын
Very well done! great amount of info for under 30 min.
@MelodySaleh
@MelodySaleh 4 жыл бұрын
Thank you! You are so good at explaining honestly.
@woillusion123
@woillusion123 6 жыл бұрын
Very great explanation! Thanks- now I get an idea of how mess spectrometry works for biomolecules.
@editingone8505
@editingone8505 4 жыл бұрын
Great video! I learned a lot, thank you for the hard work.
@tomgrev
@tomgrev 6 жыл бұрын
Thanks Matthew, this great material!!
@francescosbarbati0
@francescosbarbati0 2 жыл бұрын
Best explanation I saw. Congrats!
@sbcand7077
@sbcand7077 5 жыл бұрын
fukken brilliant m8. thanks a lot for the detailed intro.
@lorenzo.t.674
@lorenzo.t.674 Жыл бұрын
Bloody legend mate
@NORTON200010
@NORTON200010 5 жыл бұрын
Thank you!!!!
@hostanes52
@hostanes52 Жыл бұрын
Thank you so much, I definitely owe you one 👏🙌
@miloschaal2816
@miloschaal2816 11 ай бұрын
Thank you so much! I needed this for my uni course.
@user-eg4gu6sl4p
@user-eg4gu6sl4p 3 жыл бұрын
thanks so much
@15837pawimomo
@15837pawimomo 6 жыл бұрын
thank you for uploading
@timho3921
@timho3921 6 жыл бұрын
thank you so much!!!
@akhilratanmishra4213
@akhilratanmishra4213 3 жыл бұрын
elaborate explanation
@markopoloasia
@markopoloasia 4 жыл бұрын
Cheers!
@kylekookie1440
@kylekookie1440 4 жыл бұрын
Brilliant but good lord it sounds like you're presenting to the Top 30 CEOs of the Fortune 500. Get some water homie.
@MatthewPadula
@MatthewPadula 3 жыл бұрын
You don't like the sexy huskiness? ;)
@Factsraven
@Factsraven 3 жыл бұрын
This is a general theoretical on mass spectrometry. Would be more useful if top-down and bottom-up approaches in proteomics were covered.
@MatthewPadula
@MatthewPadula 3 жыл бұрын
Yes. It is on the to-do list. Hopefully in the next few weeks, these will start to be updated. They take a long time to get done properly.
@OsamaShehzad1995
@OsamaShehzad1995 5 жыл бұрын
Great video, but this guy needs to take some pauses in between!
@anjunagpal6846
@anjunagpal6846 5 жыл бұрын
How can one determine the molecular mass of protein by the peaks given in graph?
@MatthewPadula
@MatthewPadula 5 жыл бұрын
In ESI, with a single pure proteofrom being ionised, each peak will represent a different charge state of the protein, with a smaller m/z being a higher charge state (more H+ attached). To work out the mass of the protein, simultaneous equations can be used and this can be done manually with enough time. Software that comes with the MS often does this, but there is also online calculators like this one: www.bioprocess.org/esiprot/esiprot_form.php.
@temiloluwabolajioyenekan6210
@temiloluwabolajioyenekan6210 4 жыл бұрын
Hello Dr. I will like to know if you have a published paper concerning this overview because I will like to use it as one of my references for a research paper I'm writing on "Translational Proteomics with Advanced Mass Spectrometry (MS) Systems".
@mattpadula837
@mattpadula837 4 жыл бұрын
This is all out of my own head using articles and text books that were already out there, along with discussions with engineers. So I don't have a single article. If you email me, I'll try and find some srticles to send you.
@namunamu9027
@namunamu9027 8 ай бұрын
like and subscribed 👍
@sibelozdemir59
@sibelozdemir59 5 жыл бұрын
Great information, thank you, but kindly are you a smoker Dr?
@MatthewPadula
@MatthewPadula 5 жыл бұрын
Bwahahahahahahaha! No, I'm not a smoker. But the audio was recorded at about 7am in my office by myself with a headset. I realise that I need to be more dynamic.
@sibelozdemir59
@sibelozdemir59 5 жыл бұрын
@@MatthewPadula oh great, I just thought you had breathing difficulty, I would not even be speaking that early and again thank you for the videos. 😊
@medbolm4230
@medbolm4230 5 жыл бұрын
go from the simple thing, don't mix the stafs
@AGtheguitarist
@AGtheguitarist 3 жыл бұрын
Myoglobin 11:35
@rodrigosanchespires3793
@rodrigosanchespires3793 4 жыл бұрын
@16:46 What the heck is an "uncharged ion"????????
@MatthewPadula
@MatthewPadula 4 жыл бұрын
Four years and someone finally picks up a 'mistake'. You tell me what the common name for an uncharged ion is, one that is able to enter the MS, be past through the instrument and able to strike the detector, giving a false signal? An uncharged molecule, I guess.
@SethCrosby
@SethCrosby 2 жыл бұрын
The Thermo video (kzfaq.info/get/bejne/gdCFhLym3pnRo5U.html) is no longer available.
@MatthewPadula
@MatthewPadula 2 жыл бұрын
Remind me where this is referred to in the video.
@SethCrosby
@SethCrosby 2 жыл бұрын
@@MatthewPadula 20:17
@mattpadula837
@mattpadula837 2 жыл бұрын
@@SethCrosby Thanks. I will fix it for the next version.
@Creator91
@Creator91 7 ай бұрын
HOW DID YOU IDENTIFIED THESE ARE ISOTOPES OF CARBON
@kellymcgrath5541
@kellymcgrath5541 4 жыл бұрын
dada
@abdulazizabdunasimov8978
@abdulazizabdunasimov8978 3 жыл бұрын
deeeeeeeyta you say, huh?
@neilmccracken160
@neilmccracken160 4 жыл бұрын
This is a great summary but there are some serious copyright infringement issues with this video. The simulations in the LC-MS are straight from Thermo Scientific. There is no credit attributed to Thermo anywhere in the video and it is not clear that permission has been given for use of their video.
@lorigearhardt2371
@lorigearhardt2371 Жыл бұрын
Pffft
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