I am one of the people that Crispr cured. I was cured at children's hospital of Philadelphia after 28 years of Sickle cell SS! I now have more energy than ever before, my eyes aren't tinted yellow anymore, I can go out in the cold and not sickle. I feel like they just gave me a whole new life honestly. I want this to help everyone who suffers from sickle cell.

The horrible experience of watching a loved one during a sickle cell crisis being utterly helpless to stop the pain that's beyond agony I'M SO GLAD TO HEAR THERE'S HOPE FOR US IN THE IMMEDIATE FUTURE

Dylan. I know that was you yeah little bollix.

As someone who just had an organ out due to a genetic illness, I feel both hopeful and cheated by time after watching this.

Thanks for the episode, I love how DNA technology is advancing so quickly.

This is going to change humanity forever once mastered.

Gene editing might just be the most needlessly feared and underused technology of today.

"Sorry Brian, it's true" 5:32 Top left

Real Science and Real Engineering have done it again. Thank you for this amazing content! Your channels are my very favorites.

This is the best description of CRISPR Cas 9 I've ever heard.

Thank you for making concepts look simpler, the annotations and graphics are highly intuitive. Please continue to make videos on medical sciences, keeps me motivated to learn more.

Love this channel ❤️ Get to learn something new from every episode, Keep up the great work guys 🤗

This was really interesting! I wrote a 5000 word dissertation on gene therapy for pancreatic cancer this year for my EPQ (UK qualification for 16-17 year olds) so always love to hear more about this topic :)

I needed to rewatch this 3 or 4 times to understand it. It was worth it

Gene Editing sounds so crazy futuristic, man I love science so cool!

This is unbelievably amazing subject which just makes me so intrigued that I can't express!!!

Thanks you very much for your work on this video it is such an interresting subject. I espacialy enjoyed the CRISPR explanation.

This channel is freaking awesome! Totally worth the wait between uploads! Just keep it up as it is, Cheers! ;)

Wow, extraordinary work on the video! Subscribed!

Ok, so finally after a month i got to see a video from real science. This is the best science channel I have ever seen + your voice is soo good. I guess i will get a subscription for curiosity stream and get a subscription for nebula.

The future is bright if we can (responsibly) roll this out to the masses!

The future top search term for CRISPR: "How can CRISPR increase the size of my-"

My cousin has severe sickle cell disease, he has to go to the hospital multiple times per year, he has to get his blood replaced each time. Bone marrow transplants are too risky so he won’t get one.

I've been hearing about CRISPR on KZfaq, but this is the first video I've watched that clearly explains the history, and most importantly, how it actually works! Before this, CRISPR was some magic tool, but now I realize its mechanics are pretty simple

A friend of mine died of sickle cell anemia 2 weeks ago on the 15th of July 2022, which is 2 years after this video. He was only 23 years old. I feel impotent rage against this technology for not helping him, and yet I dream of hope.

Awesome episode, I love that CRISPR is actually translating to helping people in a clinical setting, which takes a lot of time in all of science from research or discovery to practical application(s), I have one small correction - gene therapy and genome editing at the level of somatic cells have existed long before widespread CRISPR-cas9 based protocols. Around 2011-2013 we got the nice CRISPR instruction manual paper(s) culminating in the ran et al., 2013 paper labs use for mammal applications of CRISPR. [1] I'm focusing on adult somatic cell mods here, which have been done in research using ZFN and TALENS, although CRISPR-based systems may promise greater specificity with less off-target effects, the main benefits IMO are the low cost and ease of access with easy programmability leading to potentially greater success rate with a low cost, i.e. widespread use and availability. It's like saying before 1960 no cars could travel at 140 mph, there were such cars, but now CRISPR can be had for a few 100 USD instead of thousands or more for a single test of gene therapy - overall treatment costs are obviously more than this, but just considering the reagents and cas proteins required. It's great that such a powerful tech is now available but I hope we can use it responsibly and recognize that CRISPR isn't a magical machine that eliminates all off-target effects. Any secondary off-target effects that hit other essential genes with a similar sequence should be thoroughly investigated for each given single gene disorder/single gene edit, and consider the effect of possibly removing code from one of the most interconnected and complex systems we know of (i.e. mammal/eukaryotic/human genome). However when used in bacteria, I am very comfortable using CRISPR because I'm not that concerned with the quality of life of my cell culture as long as they make my recombinant target and serve their purpose, but for humans even for a small and localized alteration like targeting a single gene in blood, liver tissue etc it could have profound consequences that could take a while to truly see even if your model is very sophisticated and has shown where possible aberrant cuts could be made; we can never be 100% sure what the effect(s) would be - all genes control other genes, some indirectly (like getting a cut activates the immune system, but the cut itself doesn't produce white blood cells), and many direct, like how about 1500 regulatory genes exist in humans to regulate the 20 000 or so other genes [2], so these controller genes (ie transcription factors, TFs), are used to activate a gene, and 20 some TFs must come together and regulate the activity of any single gene, so messing with one TF doesn't affect only one gene, it could a dozen genes, although there is some redundancy built in. [1] Ran, F., Hsu, P., Wright, J. et al. Genome engineering using the CRISPR-Cas9 system. Nat Protoc 8, 2281-2308 (2013). doi.org/10.1038/nprot.2013.143 [2] Ignatieva EV, Levitsky VG, Kolchanov NA. Human Genes Encoding Transcription Factors and Chromatin-Modifying Proteins Have Low Levels of Promoter Polymorphism: A Study of 1000 Genomes Project Data. Int J Genomics. 2015;2015:260159. doi:10.1155/2015/260159

Your videos are getting better and better, young lady!

i was liturally searching for crispr news because i find it so interesting. But i couldnt really find much. I am very happy that youtube showed me this video, its amazing :)

Great video! Super interesting and it’s really cool seeing the numerous applications for crispr! Just a trivial, technical question about the sickle cell treatment... Aren’t BCL genes related to tumor suppression? Wouldn’t we expect an increased risk of leukemia in patients transplanted with cells containing this BCL11A deletion? Is there a reason why this isn’t a significant concern for these treatments?

Yes yes yes yes yes yes to the nebula platform! I bow to you in deepest gratitude! I have been pressing the idea of an alternative to facebook and KZfaq since a couple years after each platform came out. But alas I am not intelligent enough to physically create either. I have been awaiting this for so long! Thank you to every single one of you brilliant contributors that pulled that off! Great video content and clear concise explanations. I'm sure I'm late as hell finding all of this but its brand new amd great to me! Thanks!

What a GREAT video! I mean, it´s just what we are used to see both over here and Real Engineering. Thank you!

I love how CRISPR went from Will cure TO Is curing.

Real engineering is the best youtube channel. No, No its real science 🤣

Dang!!!!! no mention of credit to Dr. Mojica. CRISPR mechanism and name was originally discovered back in the 90s by a particularly humble microbiologist, Francisco Mojica, Professor at the University of Alicante. He had the insight.

Thank you for your detailed explanation of CRSPR

Really good explanation of CRISPR, thanks!

Very encouraging results albeit not without issues with bone marrow rejection.I’m not sure what I find more amazing, the fact that we have figured out what the palindromic sequences function is, or that bacteria have evolved to combat virus infection by this intricate process. Is this treatment based on EDIT-301 developed by Editas ?

Deleting one line of code may solve one problem but may create several bugs - frustrated developer 😬

Yes! Can we please get more videos on this topic. A video on epigenetics would be greatly anticipated.

So informative! My new fav channel

As a sickle cell patient, this was a little dose of hope.

SCA was used to explain genetics in my HS Biology class, and it was initially caused as an adaptation against Malaria. It’s good that we know how to reverse a backfiring adaptation.

Awesome article thanks.

5:27 Shots fired.

Thank you for the information very informative

Kurzgesagt fans: Hey, I’ve seen this one! RealScience: What do you mean you’ve seen this? It’s brand new!

Real engineering got roasted 😂

I had a professor who worked on a therapeutic for sickle cell disease for years. He realized our gene editing revolution would make his therapeutic obsolete. I don't think he cares, I think he's just happy nobody will need to suffer from sickle cell disease some day soon

media needs to cover this & more money needs to be given to this type of research

This video for me finally completed what CRISPR really is. Love your videos. There is always new to learn here❤️😎.

I think this video did a good job of advocating the promise of CRISPR while acknowledging the drawbacks and unknowns that warrant rigorous caution. Good stuff! Whether through CRISPR or other means, I hope we can continue to find ways to help the sick

5:27 That Shade..!! Love it 😆

Very nicely done news piece.

Great episode, have only exploded epigentics a little but now I'm definitely motivated 😊

Good explanation, with good visual aid :)

This is a great introduction to this topic! And hooo boy, y'all just wait for what we can do in the future. Chemotherapy and a bone marrow transfer is cutting edge today, but will be seen a crude technique. Right now, we don't fully understand how the body works. We don't know what enzyme or signalling protein would grant us access into the cell to perform these edits directly. But we know a hundred times more than we did thirty years ago, and we have labs working on these questions, and we have labs learning how to create biological nano machines (i.e., proteins). I kid you not, we have a path towards curing every disease. Every. single. one. It's a long and difficult path, but we can finally see it. We probably won't get their in our lifetimes, but we will see progress. (And if we get quantum computers to a useful point, we will see a LOT of progress. The entire Rosetta@Home program would be trivialized by a quantum computer.)

Very well explained!!

I like how this video is done. Often when someone creates a brother/sister channel or hire someone to take some work off of his shoulders it suffers because the new person brings too much of his/her personality or too different a personality than I've gotten used to. Here it's very similar to real engineering. I like that.

Thank you and nice video for understanding

Saw this on a Stargate SG-1 episode after Teal'c took a bug bite from a very big bug.

Wonderfully explained.

Can CRISPR fix my relationship?

This is great - I didn't know that CRISPR had actual medical use already. The last I heard of it, I thought they'd run into the problem that the alterations wouldn't just be targeted in the right spot, but caused a similar change in many other locations as well. Maybe they overcame that problem?

doing an undergrad in genetics atm this is all so exciting of what's possible

thx u Generation x for ur work

Th upload speed and quaility of theses videos are exponential

Since I haven't seen him mentioned here, I wanna plug The Thought Emporium. He does a lot of CRISPR stuff on KZfaq and even produced a gene therapy to cure his extreme lactose intolerance. Amazing science channel! Ps great work on the vid!

Insightful and enlightening 💡

How did they reduce the off target effects of crisper in this treatment ? (Crisper has hughe off target effect rates )

Could it be that you Switched up Tom With Joe scott in the curiositystream/nebula commercial? Best regards

Man. That is complicated stuff. Amazing they can figure it out

Real Science > Real Engineering Jk...😅😅😅 They are both Great

Cool, crispr can also be used to regulate gene expression, not necessarily cut and paste stuff. Fascinating stuff.

Very informative and magical CRISPR technique.

👏to advance in gene mapping and molecular biology; btw, one suggestion please popularise the usage "disorder" (# "disease") in relation to SCA 🙏

Can you do a video on "cerebral palsey". And more about neurology...

love your work keep it uppp

This eleviates my mood despite the stuff happening in 2020.👍

nice work, keep it up

i’m hype for this channel

Please, to make life easier for Nebula subscribers, could you add a direct link to the nebula version of videos in the description of future videos? Thanks !

WOW!! This is amazing content. 10:23 the computer chip from Lain is real.....

*Feedback for the channel:* One part about ~4:26 wasn't clear to me. I would've assumed the anti-virus DNA of the cell attacks the DNA virus itself. But what the animation shows is that the virus must have already infected the cell's chromosome(s) with its DNA, and now gets removed again (or neutralised, at least). Is that correct? Does the cell DNA neutralise the virus DNA *after* it's become imbedded in the cell's DNA, instead of intercepting it before it embeds itself into the cell's DNA?

I have been reading about this CRISPR technology for years and I expected we understand and can make good things more from it then just what it can offer to us now. much more.

Well Done, Thank You!

This is great!

PAM Sequences - A protospacer adjacent motif is a 2-6-base pair DNA sequence immediately following the DNA sequence targeted by the Cas9 nuclease in the CRISPR bacterial adaptive immune system. Incase, anyone was wondering like i was!

Several times I have followed links to the Curiosity Stream website and gone through the sign up procedure only to find that there is no options for PayPal, Amazon or Google Pay. I won't use my bank details/card to sign up for one service which isn't even in my own country. Please suggest they look into more ways to pay.

would this work on sars-covid19 Rna?

1:25 I guess Pepto Bismol for everything

Isn't Crisper illegal to use on specific things?

Can this gene therapy help Cystic Fibrosis patients? I’m no expert, but i’ve always vaguely thought with CF caused by one single letter typo(mutation) it could be one of the first diseases to be treated with gene therapy.

I would really like to get a job that does stuff like this, but what exactly would the title of the job be and what would the university requirements be?

Is there a link available to watch "Our Genes Under Influence"?

Amazing!

Absolutely amazing science.

5:28 Shots fired!

Wonder how many more of these natural quirks in genetics remain undiscovered

10:04 well, idk about you but i personally find it hard to be optimistic about something ill be too dead to see. Given that we have to wait and see if they have any major side effects up til death and probably their kids, its gonna be a while before anything trickles down to the lower classes.

I have curiosity app but its password is not working with nebula app