How to Analyze GC Results for Lab

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Күн бұрын

A lesson in how to analyze gas chromatography (GC) lab results including peaks and percent composition of mixtures.
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Пікірлер: 113

@skitlles1923 3 жыл бұрын

This was so helpful! In the age of online learning this was the best thing that I came across all semester!

@jemimamoses7212 3 жыл бұрын

Thank you for this simple and clear explanation! This will help me a lot.

@hazeld8016 2 жыл бұрын

Thank you so much! In just over 10 minutes, you taught me what a GCM was and how it worked as well as exactly what I needed to know to analyze the results which neither my teacher nor my textbook nor my TAs could do for me so thank you again

@sankarasubramanian5053 3 жыл бұрын

Thanks a lot. Simple and clear explanation.

@naadiahmazlani Жыл бұрын

Thank you for making this video. it was clearly explained.

@cheyma7945 3 жыл бұрын

You're a life saver!!

@dhifarhasan6948 3 жыл бұрын

thanks a lot, that's helped me so much

@mrphysh 5 ай бұрын

I am sure that you were a very good student. I bet you got an A in every class you ever took! Good job

@Laura-hj2kk 3 жыл бұрын

really informative, helped me understand a lot more! thanks!

@ChemComplete 3 жыл бұрын

Glad to hear it!

@crazygokerff2517 2 жыл бұрын

@@ChemComplete Hi the percentage you calculated is the sum of peak Hight to assume the concentrations of product

@anitaten3856 3 жыл бұрын

thank you for such a helpful video!!!

@sabertooth_almas 3 жыл бұрын

Very helpful, thanks!

@olaleyeolutunde 4 жыл бұрын

Nice and well explained

@JonWu0716 7 ай бұрын

So helpful, thank you so much!

@crosshiiro7970 2 жыл бұрын

great video! Very informative, thank you very much

@ChemComplete 2 жыл бұрын

Glad it was helpful!

@SpencerFH 3 ай бұрын

when you describe the matching of peaks to boiling points, you put a dash in between y and z and their boiling points but not x. this makes it look like they are negative (-82 and -37) while x is positive.

@Priyanka-tt8cg 3 жыл бұрын

precise and informative

@thesealab8947 3 жыл бұрын

Excellent! cheers mate!

@HadyAkAHady 28 күн бұрын

amazing job thanks

@CienciaPelada 3 жыл бұрын

Thank you!!

@camid7403 3 жыл бұрын

this helped so much!!

@oandalevelchemistry8095 2 жыл бұрын

Good video. A point to be noted: different compounds yield different peak area even if they have the same concentration; e.g. 100ppm of X, 100ppm of Y, 100ppm of Z will not yield the same peak area. Taking only the areas to calculate selectivity/composition could yield wrong results. The compounds need to be calibrated and their respective concentrations should be used to calculate composition and selectivity.

@Alfalfaello 3 ай бұрын

I was actually looking for this comment. Thank you! It was essential to clarify it

@brittnhuli 2 жыл бұрын

great explanation! a little feedback... for the visual learners it would help to add a diagram of the standard gas chromatography setup, while youre explaining how the gas is passed through the coils. and put it next to the graph while youre explaining that the nonpolar parts are expelled first.

@ChemComplete 2 жыл бұрын

Thank you for the constructive feedback. I will consider in in future work.

@maximehendrickx6212 2 жыл бұрын

Great video!

@saka_inrmy6293 3 жыл бұрын

Thank u so much for this

@gollaprathima1134 3 жыл бұрын

Thank you so much

@Beautifullife7189 3 ай бұрын

Thank you so much it is very informative videos

@Aleu931 4 ай бұрын

Thank you

@kubra7996 Жыл бұрын

thank youuuuu for this video

@ireneaa5890 3 жыл бұрын

thank you for this video! very excellent. Can you make a video of how to analyze essential oil using GCMs

@clementinenamazzi7852 3 жыл бұрын

Thank you very much it is informative, there are samples with over 60 compounds do i do the same to get the percentage of each?

@manashjyotibaruah6758 3 жыл бұрын

This is helpful.

@georgesunnyvarkey3425 3 жыл бұрын

Thankyu so much sir

@iraqi3612 2 жыл бұрын

thank you so so much sir

@ChemComplete 2 жыл бұрын

Most welcome

@praveenmishra7863 3 жыл бұрын

nice explanation.....

@franknjmt1 3 жыл бұрын

Awesome!

@mome123 3 жыл бұрын

Really good video man! I was just wondering is it possible to use this method with a unkown sample withouth having ms or lc. And do you need to have the boiling point for all the molecules in you sample you testing before using this method ?

@georgesunnyvarkey3425 Жыл бұрын

Thanks bro ❤️

@galaxy6251 2 ай бұрын

bro stretches this out wayyyyy longer than it needed to be

@snowflakes11111 4 ай бұрын

Best video

@budia3070 11 ай бұрын

Thanks You very much, It is very good lesson for the GC understanding, but I still not clear for the area to be Calculated

@stephennguyen9476 2 жыл бұрын

Can we able to calculate of RF value when we have GC report which allow us to know retention time and area which is in μV-s? Thank you very much.

@judeonwudili9619 2 жыл бұрын

To use this 'simplistic' method, you must make it clear that you have assumed or confirmed that the 3 components have similar (even the same) response factors in the particular detector being used.

@ChemComplete 2 жыл бұрын

This is true and does need to be accounted for in more complex lab work and mixtures. This video is aimed at students first experiencing GC labs in general or organic chemistry. It would not be appropriate to teach response factors until an upper level course such as instrumental chemistry or an advanced spectroscopy course.

@rachelhughes2837 2 жыл бұрын

Shouldn't the x and y be swapped over? The peak is high so I'd of thought x would be in the second highest peak? I thought it goes in order of boiling points to high and low peaks. If that makes sense

@gbbanaag3954 3 жыл бұрын

Thanks really helpful, but if we have 2 compounds that have similar boiling point 1 cis and 1 trans which one has the lower retention time? Appreciate your feedback. Thanks

@ChemComplete 3 жыл бұрын

In GC compounds are separated by relative boiling points. Look for the lowest boiling point and it should be your first peak.

@user-md5ki5su6c 6 ай бұрын

How did you get the precise areas? Is there a table in MestreNova that shows the peak width and exact intensity that can then be used to calculate the area? Thanks

@NamesIWantAreInvalid 3 жыл бұрын

how would we adjust the ratio for the bias of a detector?

@anamikaG28 2 жыл бұрын

May I know how to calculate the area of the peak, as in real graph, the peaks are not very sharp as triangle where we could apply triangle area formula. Kindly let me know from where will I get the area of the peaks.

@terenica 2 жыл бұрын

how does this change if it is a relative retention time?

@jaykumarbhatt8841 2 жыл бұрын

Thnks sir 🙏 Very good sir👍 Sir what is the effect of column I.D and film thickness ????

@clip012 3 жыл бұрын

How about Normalized Peak Area? Normalized against biggest peak and get corrected area?

@Poolshockguy 3 жыл бұрын

GC shows 54 in area but under amount where it shows PPB it says -12,000. What does this mean

@epalanisamiepalanisami6863 2 жыл бұрын

Can we use Methanol solvent for edbileoil fame preparation?

@Beautifullife7189 3 ай бұрын

Sir how to calculate the area of any retention time

@kienphamtrung5173 2 жыл бұрын

how did you do it can you share with me , thank you

@NamrataGawai28 3 жыл бұрын

If mixture of methanol, acetone and MDC mixture which peak elute first . Elaborate your answer.

@kiranshahzadi2465 Жыл бұрын

how do you calculate values like 22.375

@sophies8107 3 жыл бұрын

isn't y=-82C the lowest boiling point? idk if thats a typo because you just say y=82 when u talk but wrote something else.

@Miyakimiki 2 жыл бұрын

He wrote the "-" as a reference to connected it to y. It's confusing because he didn't do it for "x". But it's meant just to show y - 82 to show that y is 82 (it is not -82).

@KacyKhietNguyen 4 ай бұрын

what if there is 3 possible products but the GC data only show 2 peaks what does that mean, how do I know which one is which one and what is present of not

@marcochimio 2 жыл бұрын

I am presuming that the Percent composition is by mass, and not by volume or mole fraction. Is this correct?

@tulip811 2 жыл бұрын

Hey can someone help. How can I get the quantity (in grams) from the area in % ?

@humakhan7694 2 жыл бұрын

How to calculate area then?

@carmelaeugenio1746 4 жыл бұрын

where did you get the area of retention? what is the formula?

@ChemComplete 4 жыл бұрын

Area is provided by the GC instrument. This is a random example teaching you how to utilize your data.

@AliAsghar-ue4lm 2 жыл бұрын

Percentage composition is by mole % or mass %

@cdietrich3252 Ай бұрын

(SUMMARY of text below): GC gives mole %. readings (percentage composition = mole %) (MORE DETAILS) I'm wondering this as well! I am 99% sure that percent composition for GC is by mole %. My thinking is that percent composition assumes "amount," and "amount" is always by moles. He also states in the video that "the GC does not know what chemicals you have," so mole% makes the most sense as the GC does not know anything about the masses of each component, which it would require in order to find mass%.

@andrewjustin256 Жыл бұрын

What do those invariable peaks mean, some are long and some are short?

@shyamlokhande3466 Жыл бұрын

How can we calculate the %yield of our product in reaction mixture using GC-MS, With or without taking any internal or external standard. Can anyone help??

@jackdawson7385 Ай бұрын

How do we get the value of area of retention time 3.1 minutes i.e. 22375

@aven2913 2 жыл бұрын

Is that template ACS format?

@ezekasarachifelix4471 3 жыл бұрын

Hi, can you make a video on waste engine oil analysis by GC?????

@madhurpandey5000 Жыл бұрын

Please find out the area of pics

@indrajeetw.5365 2 жыл бұрын

Thank you.. Please make a video on, how to calculate selectivity and conversion by using GC...

@sunithas5782 2 жыл бұрын

What would happen if retention times of compounds if the column temperature were raised?

@MrsLacqueredClaws 2 жыл бұрын

Hi, not the OP here, but doing GC lab work is my job, and generally speaking, if you expect a certain Rtime and your results show an Rtime higher than control (or reference), then you are looking at a similar, but ultimately different compound. RE: THC vs synthetic cannabinol

@tubetigeerr 3 жыл бұрын

does the machine automatically calculate the area? thanks

@ChemComplete 3 жыл бұрын

Often times it may, especially with more modern instruments, but not a guarantee.

@xharuu 2 жыл бұрын

how’d i get from my 8th grade chem class to this

@NanaAdjeiwaa-ei6om 8 ай бұрын

Please can oxygen be used instead of zero air?

@bariumselenided5152 2 ай бұрын

Dude, I _knew_ this instrument had to be set up to give you the integral. Idk why but our professor has us doing this some weird way where we estimate the integral by printing it out and physically measuring height and width to get a rectangular approximation. Why? Why couldn't I just be given data if the instruments give you data? And if we're going old school, why not cut out the peaks and weigh them? At least that method is cool.

@user-wp5tp2ut5n 3 жыл бұрын

but the area gives the mass or the mole percentage?

@AliAsghar-ue4lm 2 жыл бұрын

Have you got this answer ? Plz share

@erzas556 Жыл бұрын

but how to calculate the concentration using the area and height?

@ranarajeshwari9953 Жыл бұрын

Did u find it?? If u know..plz ask me..I have same problem

@jaxanax7162 3 жыл бұрын

Is the "Retention time" and "m/z" the same thing?

@mariamoubarak 3 жыл бұрын

No. "retention time" peaks is what you get from the GC. m/z is what you get from the mass spectrometry after the GC. It relates to the fractionation of the compounds into their fraction ions which is related to the mass to charge ratio of each ion that fractions from a given compound

@ClutchDaddy91 2 жыл бұрын

I know they say there are no dumb questions, but.. How is the area calculated for the compounds?

@caseypickens8592 8 ай бұрын

You first draw a baseline (you would not use the x-axis line).Then you would measure from the top of the peak to the baseline to get the height and then to get the width, you would go to the halfway point on the height and then measure the width from there. So, the equation would be A= H x W(1/2) if that makes sense.

@mona199696 Жыл бұрын

Gerate Video , but i`m a bit confused do you mean y= 82 °c Or Why is it last to avaporate ?

@mona199696 Жыл бұрын

Instate of minus 82*

@nhatle6129 3 жыл бұрын

how does z have lowest boiling point

@marianafaria4233 3 жыл бұрын

I think he meant 82°C instead of -82°C

@inemesitakpan226 2 жыл бұрын

what's the unit of the area

@ChemComplete 2 жыл бұрын

This can depend on the GC being used, but generally it is the area of the x and y axis units. So most GC will have minutes (retention time) along x and a volts (detector signal) on the y axis. So a common unit might be volts-minute.

@rachelhughes2837 2 жыл бұрын

how did u calculate the 22,375 ?

@akhileshchaurasiya9408 4 ай бұрын

Area of triangle formula put down in the peak portion

@Fummy007 2 жыл бұрын

4:09 I thought it was minus 82 and got confused

@vithenych9605 3 жыл бұрын

This does not seem right to me. Your calculation assumes the same detector response to all compounds. However this is not true. 50/50 % mixture does not generally make 50/50 % response. I would say you need to know the response factors to make the calculation.