How to Change Cell Culture Media
Рет қаралды 101,721
12 жыл бұрынThis is a tutorial on how to change culture media in a mammalian tissue culture.
@science_mbg 7 жыл бұрын
You should need to edit the title or add in description section about the cell type whether it is attached or suspension cell line. Because if it was suspension cell line, all the cell would be discarded as well by removing the media at first place.
@user-ze9ze3lg1l 3 жыл бұрын
i agree with you
@cansutacettin3024 2 жыл бұрын
totally agreed!
@funny11744 2 ай бұрын
Do you have any idea how to isolate the URNIE STEM CELLS by simple centrifugation? Usually, the stem cells are mixed with sediments in the morning urine- the best time for obtaining the urine stem cells. I resuspended the sediments -which disappeared at the next centrifugation , but it did not help to find any single urine stem cell. Usually, there are just 5 to 10 urine stem cells in 100 ml of fresh urine. I used a protocol with 500 g for 5 minutes of urine centrifugation.
@JYOtiRaNJanMANgaRaj 2 ай бұрын
Thank you so much 😊
@retna2183 11 жыл бұрын
KS LEE you mean open the lid slightly of the T-75, after placing in the incubator for air flow? It may be the T-75 where the cap has a filter, so you do not have to loosen it. Cheers
@el-yugudaabdul-dahiru39 8 жыл бұрын
The video is educative and interesting. However, if you are not of maintaining the sterility or not too sure of your working environment please revert to using flame at every point of your work.
@toshibacoast 12 жыл бұрын
good video.
@osmirod1 2 жыл бұрын
The t75 must remain with the cap on at all times except when pipetting
@abumosabelhindi5505 11 жыл бұрын
its very nice
@moonlikeyou3339 Жыл бұрын
How long do you change the medium?
@kyoung731 11 жыл бұрын
There's no need for PBS if it's you're just doing a regular media change.
@funny11744 3 жыл бұрын
Please mention what type of cells are ? Are human cell ?
@FireboltRiver 11 жыл бұрын
Shouldn't he spray his hands again with alcohol whenever he discards used pipettes under the cabinet?
@jeeva1996 3 жыл бұрын
No, that quick movement is fine
@SubhadeepD 8 жыл бұрын
great video indeed :) am just start doing my phd work .... and this video will help me :) sterile work = great data/result :) cheers :)
@HuongPham-ur1sz 11 жыл бұрын
làm sao để xem dc đây
@ibraheemomar8109 8 жыл бұрын
why are you not wearing a face mask?
@ubanakoblaise9138 11 жыл бұрын
No need to wash cells with PBS?
@12823matthewkao 2 жыл бұрын
you didn't wash the culture with PBS before adding the fresh media?
@amrutasudake7271 5 жыл бұрын
In how many days we should change media
@funny11744 3 жыл бұрын
Mention 2-3 days.
@lemyafinder289 8 жыл бұрын
i'm looking for a cell culture lab for an internship..can any one help?
@kslee8096 11 жыл бұрын
Why not open partially the T-75 in the CO2 incubator in the last part of the video?
@kannanrk100 7 жыл бұрын
KS Lee it's a vented flask
@Carl-Ernst-Otto-Kunze Жыл бұрын
@Zauberberg
@vadersfist12 11 жыл бұрын
what kind of bracelet was he wearing? is it sterile? I think not... for shame, sir. for shame.. :( not :D
@tulsijoshi1046 Жыл бұрын
If she discarded the media won't the cells go with the media?
@eversincekorea 10 ай бұрын
the cells are adhered to the flask
@funny11744 Ай бұрын
Only the dead cells go with media, the adherent cells are fine. Which is good for the healthy of last one. I think.
@BigBabySexBobOmb 6 жыл бұрын
Oh my God. So many mistakes! Hurts watching that..
@S0m31rishDud3 6 жыл бұрын
New Years Eve I was just thinking that
@derekdeadlifts2986 4 жыл бұрын
aren't they students though so they are not professionals?
@jackh9556 4 жыл бұрын
What mistakes were made? I'm only asking to make sure I do everything correctly.
@ppottokki 3 жыл бұрын
Pouring media onto cells...and no flame sterilization.. Flame sterilization is necessary while you put foreign materials into the clean bench.
@funny11744 Ай бұрын
@@ppottokki Not any materials could be flamed ! There is also cold sterilization.
@vadersfist12 11 жыл бұрын
My bad I meant to dislike this. CSU Pomona does it hood status!
@infinitefuture1828 3 жыл бұрын
You shouldn't wear socks while performing experiments
@funny11744 Ай бұрын
it depends on how skilled you are and it depends on the cells.
@vadersfist12 11 жыл бұрын
CSU Pomona does it better!!!
@Gangularis 7 жыл бұрын
Why are you wasting your time spraying the pipette packaging? You aren't going to get it close to being completely sterile, and that wrapper should never be over any of your open containers, anyway.
@funny11744 Ай бұрын
just for precaution.
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