Tissue Culture Series #1: How to Thaw Cells with High Efficiency
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2 жыл бұрынTo learn more, visit ms.spr.ly/6055w1Wkb
Tutorial demonstrating best practices for starting a fresh cell culture from frozen stocks, including cell thawing tips and tricks to maximize survival.
• Ice crystals and thaw time
• How to avoid osmotic shock
• Cryoprotectant removal and media replacement
• Thawing stem cells and primary cells
• Methods for resuspending cells
• Seeding density considerations
• Troubleshooting
@misscarebearclaire418 2 ай бұрын
I was told that if you didnt do a second spin down/resuspension that there would be remaining DMSO left over and cause cell damage. Is this true? or is that extra spin causing unneccessary stress
@funny11744 Ай бұрын
Regarding using the antibiotics for primary cells : Does is it necessary to add antibiotics whenever we change the media( let us say every 24 hours) for the first passage P0 , or it is recommended to add antibiotics just for the first 24 hours ?
@user-tp6vx5kk8m 10 ай бұрын
I could not understand what exactly happens after aspirating cells. Please explain.
@milliporesigma2407 10 ай бұрын
After aspirating the wash media, gently resuspend the remaining cell pellet in a small volume of pre-warmed culture media by pipetting up and down. Suspension volume and flask size is determined by the number of cells that were recovered.
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