How to turn any light microscope into a dark field scope

  Рет қаралды 71,942

Ambithusia

Ambithusia

10 жыл бұрын

For less than $1 US, convert nearly any light microscope into a dark field microscope. Commercial conversion kits can cost upwards of $100. This cheap, easy, fully reversible technique allows even the most basic 'scopes to be upgraded.
(c) 2014 Ambithusia

Пікірлер: 107
@alylyshua74937
@alylyshua74937 4 жыл бұрын
Dark field always strikes me because of the beautiful colors. Thanks for sharing!
@usernamemykel
@usernamemykel 8 жыл бұрын
You're the Alton Brown of microscopy. Well done, thank you. More, please.
@luapynneb3069
@luapynneb3069 6 жыл бұрын
After watching this and doing a little more searching for ideas, I 3d printed a range of sized discs to fit in to my filter holder, which is at the same place you placed your blackout sheet you made, works good to about 0.65 Objectives (4x 10x 40x). One thing I noticed on my microscope was I had to raise the condenser to sit a few MM under the slide to get the dark-field to work. Big thanks for sharing this info as you saved me £80-£100. ; )
@pmac5621
@pmac5621 8 жыл бұрын
Thank you very much Dan! I was considering a dark field, but the price was too high, so having this ability to make a lightfield into a darkfield is much appreciated.
@wispafish
@wispafish 4 жыл бұрын
Gave a thumbs up just for the 'something somthing' bit of the vid lol. Thank you for the advice, I'm going to try dark field now!
@ChrisFuchser
@ChrisFuchser 5 жыл бұрын
Thanks! Suggest simplifying by cutting the opaque spots out of black construction paper, instead of using a Sharpie marker and stacking. You can just put a dab of adhesive on the construction paper dot to attach it to the clear plastic.
@pattirayne1243
@pattirayne1243 3 жыл бұрын
Thank you! If you will pardon the pun, I found your presentation "illuminating". I just got the wrong type of microscope for what I'd like to do and you gave me so much inspiration. I'll follow and subscribe so my thanks becomes a tangible thing. Carry on!
@ewu2030
@ewu2030 Жыл бұрын
just ordered a microscope today and I was looking for ways to view darkfield on it ( quite cheap, no commercial adapter available) and with your amazing solution, it's brilliant! thanks man!
@shaileshkris
@shaileshkris 3 жыл бұрын
I have this strong urge to come down and help you maintain precision! Thoroughly enjoyed the content, great work, and I got to learn something!
@Fifita2012
@Fifita2012 8 жыл бұрын
Very good idea! Thank you so much for sharing this with us ! I am going to make my light compound microscope dark field now :D
@danieltadeubraido2967
@danieltadeubraido2967 6 жыл бұрын
Thank you very much for the video tutorial. I was just needing that for my microscope. You're great!
@StargazerFS128
@StargazerFS128 9 жыл бұрын
Well I'll tell ya that was just about the most useful vid I've seen on youtube in a long while, super job on that, I'll be scoping out plastic sheets pronto! Thx
@Seyhan60
@Seyhan60 8 жыл бұрын
We need more people like you on KZfaq, thanks! :)
@WillyDrengen
@WillyDrengen Жыл бұрын
Check out microbehunter
@Seyhan60
@Seyhan60 Жыл бұрын
@@WillyDrengen thanks, I just discovered his channel :)
@WillyDrengen
@WillyDrengen Жыл бұрын
@@Seyhan60 No problem, Microbehunter is just such a great guy.
@hsuanhuang404
@hsuanhuang404 7 жыл бұрын
Thanks, easy and simple. Keep the good work
@masellipictures
@masellipictures 6 жыл бұрын
Excellent tutorial and explanation, Bravo!
@theblogfather
@theblogfather 6 жыл бұрын
Hi, thanks for the video. I found it very informative and helpful to get more use from my microscope.
@lornelemaire5661
@lornelemaire5661 5 жыл бұрын
very nice, as an amateur I appreciate your video, keep up the good work!
@Thinkchronous
@Thinkchronous 7 жыл бұрын
Thanks for this useful Video. It confirmed by suspicion that darkfield is a very simple modification.
@majornabeel598
@majornabeel598 7 жыл бұрын
Excellent work Dan!
@Jennycantho
@Jennycantho 9 жыл бұрын
Thank you very much! I'm looking for buying new one darkfield condenser but now I am trying to create it.
@Broxine
@Broxine 9 жыл бұрын
thats just awesome. thank you for this tutorial :-) are you going to make some more videos ? you are good at it
@donaldeisenbarth5255
@donaldeisenbarth5255 2 жыл бұрын
Thank you; this I will try. I accidentally partially blocked light from the condenser with a small ruler and ended up with a partial dark field. It helped with the contrast.
@angelcaudillo402
@angelcaudillo402 6 жыл бұрын
Very very useful! Well done!! Thank you very much :)
@paulwisniewski6551
@paulwisniewski6551 8 жыл бұрын
thanks, i enjoyed your video, it was informative and concise
@martinramirez9671
@martinramirez9671 6 жыл бұрын
Excelent and funny video. I just buy a microscope and I am eager to convert it in a dark field microscope. I will try these great idea. Thank you.
@michaelrapheal4772
@michaelrapheal4772 6 жыл бұрын
Thanks for the tip!! When will you be producing another video?
@jgrafsheppard
@jgrafsheppard 9 жыл бұрын
I really enjoyed this.
@Mrtfrimet
@Mrtfrimet 8 жыл бұрын
Awesome. Best tutorial ever!
@spiritualwarriorhealer6154
@spiritualwarriorhealer6154 5 жыл бұрын
I love your midnight movie voice! LoL!!
@riccardostefani1966
@riccardostefani1966 3 жыл бұрын
great tutorial, thank you so much!!
@robertpendergast2620
@robertpendergast2620 8 жыл бұрын
Works well after some time spent with fitting. I made one a couple years ago. How about polarizing for the DIY person?
@BrittanyASMR
@BrittanyASMR 9 жыл бұрын
Fantastic, thank you!
@HighFidelityFox
@HighFidelityFox 7 жыл бұрын
Thanks! It worked very well!
@rockstomper8390
@rockstomper8390 4 жыл бұрын
Thanks Dan!!
@MICROWORLDofficial
@MICROWORLDofficial 6 жыл бұрын
Oh, thank you very much for that cool idea =)
@danielzavala3739
@danielzavala3739 2 жыл бұрын
Very clever... Now please show us how to update it into a phase contrast xD
@murielfoucart860
@murielfoucart860 7 жыл бұрын
Excellent ! Thank you so much :)
@indranilpahari334
@indranilpahari334 8 жыл бұрын
great work .
@K3Flyguy
@K3Flyguy 2 жыл бұрын
I make em from plastic bottle caps. Very rigid, no cost and easy to work with
@danielribastandeitnik9550
@danielribastandeitnik9550 6 жыл бұрын
Thanks!! I was researching here, it's not that expensive slide sheets to print on a laser printer (fortunatly I already have such a printer). I'll try printing some!
@hyperhektor7733
@hyperhektor7733 7 жыл бұрын
here this one can be done also easyand cheap, just 2 polarising filters , 1 for the lightsource and 1 for the Eyepiece (CPL Filters can be found from 3$ each in the analog cameras section on ebay for example on ebay in your country or on ebay china)
@zazugee
@zazugee 7 жыл бұрын
that's not darkfield, it's phase contrast you're appearing to be doing here.
@michaelb.1860
@michaelb.1860 4 жыл бұрын
That’s neither darkfield nor phase contrast but...wait for it...polarized light aka polarization contrast
@borissman
@borissman 6 жыл бұрын
You are a good speaker
@Robodie
@Robodie 2 жыл бұрын
"Something something something dark field..." Just waking up, thank you for the first laugh of the day! Better than a cup of coffee.
@usernamemykel
@usernamemykel 6 жыл бұрын
Terrific, thanks!
@ianhodgson2149
@ianhodgson2149 6 жыл бұрын
Much appreciated top job. Thankyou.
@marcmarc172
@marcmarc172 Жыл бұрын
Niceeee! This is a killer microscope 'hack'. Thanks!
@h7oslo
@h7oslo 9 жыл бұрын
Great vid! Good presentation, simple, inexpensive, effective! Tnx!!! rc
@lotharmayring6063
@lotharmayring6063 7 ай бұрын
olique illumination is better than dark field. Simply use LEDs putting them a bit aside on filterholder
@JohnHoranzy
@JohnHoranzy 6 ай бұрын
I have done that at low magnification. Now I have the cash for the real thing. Hope I am not disappointed with it.
@lainthdavidmartinez3974
@lainthdavidmartinez3974 4 жыл бұрын
Nice video bro.
@jc10907Sealy
@jc10907Sealy 5 жыл бұрын
Genius! I’ll use it thx
@magdastoksik1384
@magdastoksik1384 9 жыл бұрын
Great, for the beginner to understand the case - super!!! What about polarization and DIC? I just bought a microscope and all that extra stuff is so expensive and i have no idea how to apply it :-) i am gratefull for help.
@mammanemma5352
@mammanemma5352 Жыл бұрын
Sir you are a wizard
@senanlane6882
@senanlane6882 4 жыл бұрын
Thanks
@punishalltrolls
@punishalltrolls 8 жыл бұрын
The field can be darkened further by enlarging the shaded diameter. Can we use a similar technique for solar filters on telescopes?
@PEARLING-EXCELLENCE
@PEARLING-EXCELLENCE 4 жыл бұрын
Nice information, do you have any trick for inverted microscope to turn them in phase contrast such easier way?
@rafaassuncao5433
@rafaassuncao5433 3 жыл бұрын
Thanks sir!
@Samsasi77
@Samsasi77 9 жыл бұрын
thank u ...so help ful msg ...keep it up ..
@joewebster903
@joewebster903 5 жыл бұрын
Excellent
@healthesoils
@healthesoils 3 ай бұрын
soooooo good thank you 🙏
@funny11744
@funny11744 3 жыл бұрын
Nice video ! Nice person.
@ellencalabrese5308
@ellencalabrese5308 4 жыл бұрын
Thankyou
@christinahinton4538
@christinahinton4538 3 жыл бұрын
My thumbs up came at the something something something part😂😂😂
@JamieDelour
@JamieDelour 8 жыл бұрын
Hi Dan! What kind of microscope would you recommend a beginner that wants to view red blood cells under darkfield? Thanks for this DIY!
@haveaniceday7950
@haveaniceday7950 Жыл бұрын
This is my question as well. Did you ever find the answer?
@migmagingenieria
@migmagingenieria 6 жыл бұрын
Thank you so much for your vid. Please give me some advice, I am growing some lactobacillus cultures (now trying to replicate some L. Reuteri). I would like to be able to see the action and make videoclips and pictures. Would an Omax 40-2500x work, is that enough amplification??? Good luck!!
@PrasetyoMuhammadDwiBiology
@PrasetyoMuhammadDwiBiology 8 жыл бұрын
omg we need this :D
@Sara_London
@Sara_London 4 жыл бұрын
This might be a silly question but could you do something like this with a flashlight and jewlers loup. By covering the light from the flashlight with the black plastic and looking thru the loup? I know nothing about microscopes! I still have a lot of learning to do. I've been looking at gems as a hobby. I stare at them for hours with different magnification loups and a flashlight. I keep hearing about "dark field illumination" when I watch gem identification videos on KZfaq. Until I can get my own microscope I thought maybe this might be a very rough diy way! Thanks for the video... now when I do get a microscope I'll know how make my own dark field :) Plus it was funny! Nicely done!
@eqlzr2
@eqlzr2 5 жыл бұрын
How do I know when I have the right diameter for the light block? Thx.
@MasudO313
@MasudO313 4 жыл бұрын
Nice, i need help or direction. I want to see water clusters. Is thier a way.
@philiptaylor2567
@philiptaylor2567 7 жыл бұрын
I tried this method to look at the spirochetes in my blood, and it worked well at 10x. But when I try it at 100x with immersion oil it doesn't work. The image shows up faded and looking like a poor lit version of a light field microscope. Do have any ideas why this would be, and how I can make this work for high power with immersion oil?
@wesremy
@wesremy 7 жыл бұрын
Phillycheesecreampie Cake u need a smaller cirkel for bigger magnifications
@wesremy
@wesremy 7 жыл бұрын
Phillycheesecreampie Cake no just kidding u cant get darkfield with high aperture objectives
@philipwilkin2667
@philipwilkin2667 7 жыл бұрын
Uhm... Why are there spirochetes in your blood?
@vortexor1
@vortexor1 7 жыл бұрын
Did you get it managed to see good pictures of the blood in 100x?
@petetheweet
@petetheweet 6 жыл бұрын
Great idea, thanks for sharing. Wouldn’t it be quicker and easier to glue something opaque onto a single plastic sheet instead of colouring several sheets? Or am I missing something?
@Nihaikitty
@Nihaikitty 5 жыл бұрын
I was wondering this as well.
@michaelb.1860
@michaelb.1860 4 жыл бұрын
Yes that would work just as well - opaque disc should be as thin as possible
@thedealer799
@thedealer799 Жыл бұрын
I wonder if you could just paint the coin you use black and glue it to the transparent material.
@ZanteF12R
@ZanteF12R 8 жыл бұрын
How do you film the microscope? do you have a usb lens or you put the camera in front of the eyepiece?
@ravenm.4396
@ravenm.4396 4 жыл бұрын
I love you.
@Metalsenpai
@Metalsenpai 4 жыл бұрын
Thanks. That´s only works in 5x and 10x. Someone know how to make that in 40x or 100x? I discovery a very easy way to do that , with 5x and 10x, with a specific kind of filter suporter: open the diaphragm and move the filter suporter to the right until you get the image. Will work too.
@gabrielyosei7565
@gabrielyosei7565 3 жыл бұрын
I have the same problem with 40x
@haveaniceday7950
@haveaniceday7950 Жыл бұрын
What would be a good low cost scope for viewing live blood?
@alexpopblock6435
@alexpopblock6435 4 жыл бұрын
can't you just colour a paper circle in black and cover it in plastic circles
@rockstomper8390
@rockstomper8390 4 жыл бұрын
Meet Dan, He is a BAMF!!
@ellencalabrese5308
@ellencalabrese5308 4 жыл бұрын
I gotta look at some mites from the dog
@jackemled
@jackemled 8 жыл бұрын
THANK YOU!!! I FINALLY FOUND SOMETHING ON MAKING A DARK FIELD FILTER!!! **Explodes.**
@disasterexperiments493
@disasterexperiments493 4 жыл бұрын
Asewome!!!
@DianneElizabeth64
@DianneElizabeth64 2 жыл бұрын
So it’s not true or true because of strength that only Arizona & Nevada allow dark field microscopes
@benibuntu2052
@benibuntu2052 Ай бұрын
❤❤❤ ⚘️🌷⚘️. .Thank You Verry Much..🙏🙏😊😊😊👍👍👍
@miguelelento5637
@miguelelento5637 5 жыл бұрын
Brightfield
@ahmedraza1587
@ahmedraza1587 2 жыл бұрын
That guy used is Brain 🧠= 110% 😅
@MrBrianlowdown
@MrBrianlowdown Жыл бұрын
Somthing Somthing Somthing...not cheap...Ha!!! You can say that about so many things today...
@christiandietachmair334
@christiandietachmair334 6 жыл бұрын
I think 7 don´t like this Idea becauce they are selling Darkfield Microscopes .
@abergethirty
@abergethirty 6 жыл бұрын
What is with your odd enunciations ?
@picometer472
@picometer472 3 ай бұрын
stop waiving your hands in the face of the viewer--it;s offensive
@ravanabrahmarakshas4263
@ravanabrahmarakshas4263 4 ай бұрын
brilliant. you saved me a few hundred dollars. thanks.
@rezzer7918
@rezzer7918 Жыл бұрын
Moronic bordering on bonafide lunacy
@spondoolie6450
@spondoolie6450 Жыл бұрын
I am here from the future to tell you to buy Dogecoin.... you'll tank me later
@angelcaudillo402
@angelcaudillo402 6 жыл бұрын
Very very useful! Well done!! Thank you very much :)
🔬 Making microscope filters: Darkfield, Rheinberg, Oblique
21:22
Microbehunter Microscopy
Рет қаралды 59 М.
Nutella bro sis family Challenge 😋
00:31
Mr. Clabik
Рет қаралды 14 МЛН
I Can't Believe We Did This...
00:38
Stokes Twins
Рет қаралды 119 МЛН
How To Turn Your Microscope Into A Dark Field Microscope
3:45
My Microscopic World
Рет қаралды 10 М.
🔬 How to use the condenser aperture diaphragm | Amateur Microscopy
10:14
Microbehunter Microscopy
Рет қаралды 35 М.
Stomatal peel
6:17
Alan Harvey
Рет қаралды 218 М.
Microscopy: Dark Field, Phase Contrast, Polarization and DIC (Edward Salmon)
31:29
How to use a microscope and oil immersion
13:14
bioprofaz
Рет қаралды 465 М.
Dark field condenser
12:34
Clive Bagshaw
Рет қаралды 10 М.
Installation of Dark Field Condenser on i4 Microscope
11:16
LWScientific
Рет қаралды 1,1 М.
Lecture 9: Dark field and Phase Contrast
15:14
Joseph DeGiorgis
Рет қаралды 2,6 М.
How To Use A Phase Contrast Microscope
2:32
Microscope Central
Рет қаралды 8 М.