Hydrophobic interaction chromatography ( HIC)

Hydrophobic interaction chromatography ( HIC) | Application of HIC

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Күн бұрын

Hydrophobic interaction chromatography (HIC) separates molecules based on their hydrophobicity. HIC is a useful separation technique for purifying proteins while maintaining biological activity due to the use of conditions and matrices that operate under less denaturing conditions.

Пікірлер: 69

@qetsiyah1766 4 ай бұрын

The visuals are great! They were so helpful for my understanding. Thank you.

@animatedbiologywitharpan 4 ай бұрын

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@valentinascatizzi6406 3 жыл бұрын

Clear and fluid explanation, thank you . greets

@animatedbiologywitharpan 3 жыл бұрын

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@Nick.5.9..175 2 ай бұрын

You are amazing! Thank you so much

@animatedbiologywitharpan 2 ай бұрын

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

@qamaral-ubiedi5404 3 жыл бұрын

Do you know how long should my HIC column be to isolate GFP from 1g e.Coli?

@vg2358 8 ай бұрын

Great explanation, thank you very much!

@animatedbiologywitharpan 8 ай бұрын

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@animatedbiologywitharpan 8 ай бұрын

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@SS-lr6uk 4 жыл бұрын

Thank you sir it was much needed.

@animatedbiologywitharpan 4 жыл бұрын

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@nareshbarik5384 2 ай бұрын

Thank you ❤

@animatedbiologywitharpan 2 ай бұрын

could you please help me by sharing my contents with your friends

@shivaniathani8082 3 жыл бұрын

All the videos on this channel are very very useful. Thank you.

@animatedbiologywitharpan 3 жыл бұрын

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@suvismaya5980 3 жыл бұрын

Thank u so much sir I had problem in understanding hw they will use HIC for virus purification...now it's clear......😊😊😊😊

@animatedbiologywitharpan 3 жыл бұрын

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@suvismaya5980 3 жыл бұрын

@@animatedbiologywitharpan sure😊

@user-rw6fe3vm5b 5 ай бұрын

great

@animatedbiologywitharpan 5 ай бұрын

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@JJH2004 3 ай бұрын

So basically the hydrophobic analytes take longer to be detected as they interact more with the hydrophobic column?

@animatedbiologywitharpan 3 ай бұрын

Yes the elution time for hydrophobic molecules are more

@maninderdhillon8351 3 жыл бұрын

Excellent.. presentation .

@animatedbiologywitharpan 3 жыл бұрын

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@maninderdhillon8351 3 жыл бұрын

Sure.. I will do it.. but it will inevitably reach out to millions one day... Keep the good work going..

@animatedbiologywitharpan 3 жыл бұрын

support from audience is my motivation....hope to bring nice content soon

@KentJustinBanguilan 8 күн бұрын

You are so smart :)

@animatedbiologywitharpan 8 күн бұрын

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

@srijandey87 3 ай бұрын

Very well explained ❤

@animatedbiologywitharpan 3 ай бұрын

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

@sh7arwt3teer99 2 жыл бұрын

please if you can explain to me, why the mobile phases containing a concentration of ammonium sulfate promote the binding of many proteins to HIC columns??

@blueyolker4961 2 жыл бұрын

i forgot how it worked but i think it had to do with ammonium (NH4) being a keypart of proteins

@davidrosen5137 Жыл бұрын

Super helpful.

@animatedbiologywitharpan Жыл бұрын

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@bioline8441 3 жыл бұрын

so good.

@animatedbiologywitharpan 3 жыл бұрын

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@shrutikachaudhary672 3 жыл бұрын

Little bit clarity is needed while speaking rest the video was really nice and explanation through diagrams is good.... Keep it up 👍

@animatedbiologywitharpan 3 жыл бұрын

Thanks for the feedback....these constructive criticisms would help to improve the quality in future

@animatedbiologywitharpan 3 жыл бұрын

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@sasukeuhiha1131 2 жыл бұрын

I used to have a problem understanding HIC but watching this video has made me realize that I also have a problem understanding English.

@HectixShorts 2 жыл бұрын

Another nice effort!

@animatedbiologywitharpan 2 жыл бұрын

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@himankjain5473 4 жыл бұрын

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@animatedbiologywitharpan 4 жыл бұрын

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@animatedbiologywitharpan 4 жыл бұрын

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@rukhsarfatima2283 7 ай бұрын

How can water molecules mask the hydrophobic surfaces???

@songohan393 2 жыл бұрын

Thannnk you

@animatedbiologywitharpan 2 жыл бұрын

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@aparnaaa273 4 жыл бұрын

Need to increase volume a bit . Thanks for the vdo

@animatedbiologywitharpan 4 жыл бұрын

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@animatedbiologywitharpan 4 жыл бұрын

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@tamanaaswal5316 2 жыл бұрын

Moj krdi sir mst explanation

@animatedbiologywitharpan 2 жыл бұрын

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@animatedbiologywitharpan 2 жыл бұрын

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@richajoshi2578 4 жыл бұрын

Nice but plzz make videos on animal and plant biotechnology important topics

@rashmiranjansahoo3975 4 жыл бұрын

Explanation could have been more better with written texts.

@nanhelalgupta476 4 жыл бұрын

please does better in which don't mine time limitation..

@animatedbiologywitharpan 4 жыл бұрын

Thanks for the suggestion

@animatedbiologywitharpan 4 жыл бұрын

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@hammadak8 4 жыл бұрын

i think u have problem in your voice... kindly focus on it. and content is good but only voice.

@animatedbiologywitharpan 4 жыл бұрын

Ya my mic was not working properly

@hammadak8 4 жыл бұрын

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@_CatalystGuy 9 ай бұрын

Please speak a little bit loud😢

@animatedbiologywitharpan 9 ай бұрын

Yes , in this video the audio is not good. May be I should make a new video

@kuldeepbiotech4014 3 жыл бұрын

Such a low pitch sound really irritating and such things makes the learning distracted. Audio quality always matters when we try to maintain concentration.

@animatedbiologywitharpan 3 жыл бұрын

I took your comment positively....the sound quality would be improved in future