Inoculating Liquid Bacterial Culture

Рет қаралды 159,928

Күн бұрын

Inoculation is the process by which you introduce bacteria into liquid media. In this video we will demonstrate best practices to perform inoculation, ensuring sufficient numbers of bacteria for plasmid DNA isolation.
For the full written Inoculations protocol, visit:
www.addgene.org/protocols/ino...
For our DNA Isolation Protocol, visit:
www.addgene.org/protocols/pur...
For our Creating Bacterial Glycerol Stocks protocol, visit:
www.addgene.org/protocols/cre...
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0:00 Intro
0:48 Materials
1:29 Setup
2:34 Inoculation
4:34 Incubation
5:34 Post-Incubation
5:43 Troubleshooting

Пікірлер: 27

@addgene Жыл бұрын

Addgene does not regularly monitor comments posted here, so we may not see your question immediately. We’d be happy to answer any questions sent to help@addgene.org as soon as possible. Please include the name of the video along with any questions so our support team can help. Thanks!

@lovelysoni1829 5 жыл бұрын

Nice video for kind information thanks again very much to you

@koalasaw 3 жыл бұрын

very helpful thank you

@laxmikiruthikamanoharan1768 4 жыл бұрын

Thank u

@akshitasoni445 Ай бұрын

Should i use nutrient broth instead of LB for Bacterial DNA Isolation @Addgene

@TALKyLIKEENGLISH Жыл бұрын

so we should after transformation: liquid medium --> plate --> liquid medium. What if we skip the first step?

@jasonbattermann9982 5 жыл бұрын

Why do so many education institutions call LB lysogeny broth, but companies use the term luria broth?

@impulsessj2033 5 ай бұрын

luria is the person who created the formula.

@funny11744 3 жыл бұрын

Please provide here the link with quantities of antibiotics for culture . Thanks

@addgene 3 жыл бұрын

Thanks for watching! You can find a chart of antibiotic concentrations here: www.addgene.org/protocols/inoculate-bacterial-culture/

@nitishkumarprajapati7115 Жыл бұрын

How to culture Sporosarcina pasteurii Bacteria?? Pls make video....

@devanisasankan818 3 жыл бұрын

👍👍

@jejeoluwatimilehin8918 Жыл бұрын

in a situation where we use gentamycin, kanamycin and rif why is this?

@janebond3263 Жыл бұрын

how to storage with glicerol?

@nikilee8862 Жыл бұрын

why do you need antibiotic?

@anjalisharma314 2 жыл бұрын

Sir I have to incubate an organic liquid of 50mg/L with a bacteria. Please tell me the procedure for it.

@addgene 2 жыл бұрын

Thank you for your question! You might find the protocol associated with this video helpful www.addgene.org/protocols/inoculate-bacterial-culture/

@anjalisharma314 2 жыл бұрын

@@addgene thanks for response sir but I want to know the degradation ability of a particular compound by a particular bacteria. Shall I apply the same procedure, that has been given in the link.?

@addgene 2 жыл бұрын

@@anjalisharma314 The protocol shared above is intended for growing up bacteria in liquid cultures and may be a good starting reference. However, Addgene does not perform functional experiments like the one you've described so we can't provide specific recommendations on the conditions you may need to use. Sorry we can't be of more direct assistance!

@anjalisharma314 2 жыл бұрын

@@addgene it's ok sir?

@impulsessj2033 5 ай бұрын

my mans streaked it so wrong. you cant scratch the agar like that

@oulythorng3922 2 жыл бұрын

Why don’t we take back the tooth stick out of the medium ?

@addgene 2 жыл бұрын

Thanks for your question! Leaving the toothpick in the culture tube avoids having to stick something else in the tube to remove it (e.g. tweezers), which could lead to contamination. Removing the toothpick once you've finished growing the culture is less risky because any contaminant won't have time to grow.

@Mirracle74 Жыл бұрын

Elon Musk wants to inoculate the surface of Mars with H. sapien. I think that it’s a lousy idea because Mars isn’t the right growth medium for humans.