NOT ALL HEROES WEAR CAPES! You're saving lives here. Respect.

THIS WAS PERFECT!!! I even clapped at the end! GOOD JOB!

Thanks you!! Why can't teachers explain that well!?! Life saver!

This was the height of perfection in teaching.. Thank you so much Sir... for making such difficult topics easy to understand.. love all your videos ..:)

Outstanding. These concepts aren't always that complicated, but they are often explained so obtusely that it hinders comprehension. I think it is a skill to be able to communicate these things without such obstructions and you guys do this consistently.

You are a really good speaker and teacher. I understood everything 100%. Subbed. Thanks!!

Your videos are so helpful. Have been watching these for years and I'm almost through university. Thank you!!

Currently im studying at a medical college in Republic of Korea, and honestly you are a true life savior Thx a lot :) Ps. After watching this lecture, eating a chocolate chip cookies :D

I can't even put in words how blessed I am to discover this channel, tysm💗.You are a big time saviour !!!

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I've watched your lecture on Gel Filtration Chromatography as well as this one and you explain the processes SO much better than my PhD University lecturer does so thank you!

This was such a perfect explanation! I loved the analogy too - I wish you were my BioChem professor!

All of the videos that I've seen by AK Lectures are well very done and SO HELPFUL. You do a great job, thanks!!

Best biology teacher in the world . No one can better than AK lecture

I forgot everything of chemistry after graduation. At first, i tried to get glimpse of this because there were so many words.. However, your explanation was so clear and i already watched your other lectures. I'm really appreciated with your explanation.

Wow, simply outstanding! You present this material so clearly and so well! Thank you so much!

Gracias por esto, que simple y facil de entender lo haces. Veo tantos comments de gente agradecida. De verdad eres un heroe, mis respetos y muchas bendiciones para ti. Saludos desde Puerto Rico.

There is no explaining this any better! Great job sir! Lesson learned. Now I cannot get this out of my head…

Thank you sir. I study molecular biology in austria and english is not my native language, but you explained this stuff better than all the german versions i´ve ever listened to. You are very talented. Tanks alot.

YOU HELPED ME THROUGH 3 QUESTIONS ON MY PAST EXAM! THANK YOU!!!

The best and simple explanations in the world, i study microbiology and your video was very helpful, THANK YOU !!

You're amazing, really amazing! I swear I didn't understand a word at school (in which the teacher spent at least 1 hour explaining), and after some time when I wanted to revise I didn't understand a word either in my copies. When I discovered you made this lecture I literally jumped from happiness. After 5 mins from watching, I understood everything. Just wow... I'm really really amazed and flabbergasted at how you could explain so well. Thank you so so so much!

You are amazing and your teaching is so perfect.. Thank you for this

You open my eyes ! Thank you for this hard works indeed! Love this !

WOOW I have a lab today about this and I didn't get what was written so searched on KZfaq. You really explained everything so well. Thank you soso much :)))) Great video really.

this is called PERFECTION! saves a lot of time by watching this

You have videos for everything I need for this course I'm having!

Thank You SOOOOO much! I have a Biochem exam on Monday and your videos are extremely helpful and informative!

Best ever teaching method👏 👏 Thanks for making this subject interesting..

the way you explain things is simply awesome

Excellent video that I have seen about ion exchange chromatography, thanks a lot.❤

BEST BEST! I was searching this answer for the Whole Day, yes whole day. And here it was Thank you

You always make things look very easy and simple , you are a great lecturer !!!! thanks !

Thank you so much for making these videos, they are so helpful and you are such an amazing teacher. Im able to understand these concepts clearly and concisely. I appreciate you too much, thank you!

Ak lectures is my favorite channel. by the inspiration of this channel i started my channel covering all topics regarding pharmaceutical analysis & biochemistry. i did video on ion exchange chromatography

Exceptional level of teaching, really grateful.

ما شاء الله , دقة الفيديو عالية ,, أبدعت بالشرح ,, شكرًا

OMG this was so good I didn't get the lecture slides but after watching your video, everything is SO clear!!!!!!!!!!!!

Clear and quick explanation! Thank you. Just what i needed.

Chocolate chip cookie analogy was brilliant. Just finished watching 34 videos, and you got 34 likes :) More to come.

Part with chocolate cookies - super!! Thank you for very very nice lecture!!

Explained perfectly, thank you!

thanks,man! prepared for my exam now due to ur explaination 😉👍

Thank you so much for making these lectures so simple and easy to understand!!! I'm suprised you have only around 550 views until now :P

That was great. Thank you very much, I do think I'll be using your videos for help from now on! Biochemistry has proved very difficult this semester.

Great video, and great analogy with the chocolate chip cookie kids! Thanks for this :)

The example and explanation is marvelous. Really well explained. Please include a bit of information about its industrial applications, its advantages and disadvantages. Thank you.

Omg, this explanation was awesome! Thanks man, very helpful.

awesome teaching style!! wont forget this concept ever!!

You remind me of leonardo dicaprio in wolf of wall street - your sound and mannerisms! Anyways - thank you- this is brilliant

I am the kid that will stop for the chocolate chip cookies! Well presented btw!

Very good job ,I really like this site . Analysis made easy henceforth.

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Your videos are so helpful . tnx

100% understood. well done sir your all lectures are very very helpful.

Thanks much, u really great , simple teaching and clear voice , i have only one request , if u can zoom alittile on the board

Thank you so much for doing this for free.. you are awesome

subscribed because of the examples you gave.. the chocolate cookies... great explanation!! helped a lot for my exams.. :)

Amazing explanation!!!!!! It helped me so much!!!!!

This was beautifully explained. THANKYOU‼️‼️

Art of teaching.

U did areally good job.... Many thanks

nice teacher.thank you very much for your work. only god will reword you

Thank you kind sir, excellent review for tomorrows lab!

While mass of any protein is not 0, suggesting that gravitational force pulls it down the column is a bit misleading to say the least. Also when you have electrostatic attractions between opposite charges in the column it is silly to suggest that anything that is electricly attracted to the stationary phase in the column will ever move down the column, unless you balance the charge between the stationary phase and that particular protein or whatever species you're trying to purify.

Perfect explanation!

This was so incredibly helpful! Thank you so much

Thanks so much! You made it easy to understand!

Very educational and helpful. Thank you!!!

Your explanation is great I love it 😍

That was a really good analogy

so much thank you , your videos and explanations is really helpful!!!

I like what you are saying and the tempo is good in your videos (i have only watched a few about chromatography)! On your previous videos about chromatography, it seems to me that you are shouting when talking. But in this video you are more calm - stay like that! :) cheers

A very good presentation. I have though a question that may be basic. It is regarding the competition of charged salt components with attached proteins in the column medium/resin. I can understand that salts will be attracted by the opposite charge of the resin. However, because they have bigger ionic strength will they manage to disassociate the already bound proteins, break the existing ionic bond? Moreover, some proteins will be eluted but I imagine that a percentage of them will stay in column. Thank you very much in advance!

This helps me to visualize the words I'm reading in textbooks. It really is helpful thank you so much!! That asides, who in the world hates choc chip cookies!!! They are delicious :)

Thank you for this! God bless you!

Dude you've saved my life

Really clear explanation! Thank you so much!

Best explanation!

Does adding the salt solution create the salting out effect? Thank you for the insightful lecture!

I hardly ever comment but holy shit I learned something that my professor couldn't do all semester long.

your lecture helped me a lot THANK YOU SO MUCH!!!!!!!!!!!!!!

Now I can only think of biochem when I'm eating chocolate chip cookies. Thanks for the vid!

bless your soul, thanks to you I will make it through my exam :D

EXCELENTE, FELICITACIONES! SE ENTIENDE PERFECTAMENTE EL INGLES!!!!

perfect refresher for my job interview...😃

Please keep doing what you are doing! You are great :)

you make everything so easy thank you soooooo much

Thanks a lot for such great lecture. It is helpful and easy to understand. I would like to know, will the salt solution not have an effect on that protein?

Nicely explained ! Thanks very much! One doubt - what will be the effect of pH change of the solvent ( mobile phase) on the separation / on the bead charge ??

I understand everything now, thank you so much !!!

A JOB WELL DONE, YOU DID WELL.

could you also please label which one is the anion and which is the cation exchange?

This was so helpful

perfect and easy thank you!

Clearly explained 👍👍👍

Very nice and helpful

Thaaaank you very much. really good presentation.

hi, thanks for the clear explanations. I have a doubt- How do we know when it's time to collect the respective eluate 1, 2 and 3? as in real life, the solution would be all same in colours.

This is great! Thanks for posting this lecture!! I am an engineer and would like to learn more abt Ion Exchange chromatography. Do you recommend any book or online article? Thanks!