In this video, Petrus Siregar from Chung Yuan Christain University will introduce how to conduct laser-based skin ablation techniques and study skin wound healing in zebrafish. After imparting wounds to the skin of the zebrafish, wound images were collected and used for deep-learning training by convolutional neural networks (CNNs) to calculate the area of the skin wounds in an automatic manner. The laser-based skin ablation and deep learning-based wound-size measurement methods reported here provide a faster, reliable, and reduced suffering protocol to conduct skin wound healing in zebrafish for the first time.
Original paper: Siregar, P.; Liu, Y.-S.; Casuga, F.P.; Huang, C.-Y.; Chen, K.H.-C.; Huang, J.-C.; Hung, C.-H.; Lin, Y.-K.; Hsiao, C.-D.; Lin, H.-Y. Optimization of Laser-Based Method to Conduct Skin Ablation in Zebrafish and Development of Deep Learning-Based Method for Skin Wound-Size Measurement. Inventions 2024, 9, 25. doi.org/10.339...