Presenter:
Lauren Jachimowicz, Ph.D.
Application Development Scientist, ACEA Biosciences, Inc.
About this webinar:
Accurate measurements of intracellular Ca2+ concentrations allows for a more comprehensive understanding of Ca2+ regulated cell functions and signaling pathways. Ca2+ is an important molecule that affects a wide range of physiological and pathological processes. Unlike other secondary messengers, Ca2+ is not synthesized or metabolized, but stored and rapidly released by channels/pumps that maintain Ca2+ concentrations in distinct cellular compartments. Flow cytometry allows real time measurement of a calcium flux response utilizing fluorescent Ca2+ indicators which exhibit an increase in fluorescence upon binding to Ca2+. In this webinar we will discuss the basics of calcium flux measurements by flow cytometry, providing specific details on experiment setup, acquisition, and analysis on the NovoCyte flow cytometer.
Key topics to be covered:
1. Essentials of calcium measurements by flow cytometer
2. Calcium flux experimental setup and acquisition on the NovoCyte
3. Detailed analysis of calcium flux in a T cell line using calcium indicator dye Fluo-4/AM