Making Luminol

Рет қаралды 967,502

8 жыл бұрын

Hey guys, this is the last part of the luminol synthesis. It has been a long time coming and I am sorry for the mega delay!
Luminol Recipe from: www.chm.bris.ac.uk/webprojects...
3-nitrphthalic acid synthesis video: • Making 3-nitrophthalic...
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Пікірлер: 652

@RiddimDubstep 6 жыл бұрын

Great! Now I can make luminol and test if I cleaned the blood enough.

@JakeDoesDaMcPe 6 жыл бұрын

Riddim Dubstep 😂

@nunyabisnass1141 5 жыл бұрын

Muriatic acid is pretty good at cleaning blood from semi porous surfaces like concrete, but it will also attack the concrete. After that sodium hydroxide can be used to destroy trace proteins.

@DryLog420 5 жыл бұрын

@@nunyabisnass1141 thanks! Exactly what I needed ;-D

@nunyabisnass1141 5 жыл бұрын

Jimmy Tremblay yeah just so we're clear, you're a hunter, and you hang the carcass in your garage...right? Lol

@DryLog420 5 жыл бұрын

@@nunyabisnass1141 call me a... contractor... of sorts... I do hang the carcasses in my garage, thanks for asking :-D

@NurdRage 8 жыл бұрын

excellent work! way better and cleaner than my approach.

@NileRed 8 жыл бұрын

+NurdRage Damn, that means a lot coming from you. I also think this is the first time that you've commented on a video of mine :)

@101onzlow 8 жыл бұрын

+NurdRage you guys should do a collaborative video together! That'd be amazing to see!

@laserfloyd 8 жыл бұрын

+Nile Red It's funny thought that NurdRage was you and you were saying that your approach was better than... your approach. It's the NR initials. ;)

@drmed92 8 жыл бұрын

+Bane? NR means No Reaction

@wyldeman0O7 8 жыл бұрын

+NurdRage I totally read this in your voice!

@Wind0171 8 жыл бұрын

If you ever make t-shirts, one idea is to have the words, "We'll just have to pretend that it's there."

@ausblob263 5 жыл бұрын

i think an "anyway" shirt would be awesome too lol

@garywang4999 5 жыл бұрын

"ice old ethanol" tshirt would be the best lol

@johnapple6646 4 жыл бұрын

"Wash it with distilled water"

@krissp8712 4 жыл бұрын

"In hindsight, I should have used a larger container"

@wayneyam1262 4 жыл бұрын

@@krissp8712 "the yield is extremely low, and I don't know why"

@thelionsshare8530 4 жыл бұрын

me: where have I heard luminol before.....??? me: (remembers my Ace Attorney phase) me: ah. right.

@jarlesleglerg9064 4 жыл бұрын

Which one? DD?

@civilixxxx8914 4 жыл бұрын

I think luminol was introduced in in last case of first game, and reappeared in fourth game and ME:AAI spin-offs (and I think it should appear once more in sixth game)

@Rina-kd8hf 4 жыл бұрын

mood ngl

@davidfrischknecht8261 4 жыл бұрын

I heard about it when I was watching CSI.

@shyyyyyy1904 3 жыл бұрын

I was thinking the same thing

@AmusedToast 7 жыл бұрын

I really hope you do a video of luminol reacting with blood. I am currently working towards a Forensic Science major at Wichita State University, and am highly interested in chemistry as well, so I'd love to see a video that applies to both :D

@HooyahPeacock 4 жыл бұрын

You still in Wichita

@AmusedToast 4 жыл бұрын

@@HooyahPeacock I sure am.

@HooyahPeacock 4 жыл бұрын

Me too

@AmusedToast 4 жыл бұрын

@@HooyahPeacock That's cool. I'm actually still at WSU for forensic science. I'm getting my bachelor's at the end of the year.

@joegarza3445 2 жыл бұрын

@@AmusedToast COME TO LOS ANGELES, WE HAVE PLENTY OF COLD CASE FILES TO SOLVE, INCLUDING Y FRIEND LUCIA, WHO SUPPOSEDLY SHOT HER SELF IN THE HEAD WITH HER SHERIFF BOY FRIEND, HE WAS ALWAYS A TOTAL JERK OF A GUY, AND I NEVER TRUSTED, OR RESPECTED HIM AS A PERSON. LUCIA HAD A TWO YEAR OLD, THAT SHE LOVED TO DEATH, I KNEW LUCIA WELL, LEA REMEDY JUST MOVED TO HOLLYWOOD SCIENTOLOGY, TO PURSUE HER ACTING CAREER, AND STARTED TO HANG OUT WITH US AT LUCIA’S HOUSE, AND GOT TO KNOW WHAT A JERK LUCIA’S SHERIFF BIY FRIEND WAS. I BELIEVE IT WENT DOWN AT HIS HOUSE IN STUDIO CITY. RIP LUCIA BENTTONCOURT.

@_mels_ 8 жыл бұрын

Luminol produces a nice blue glow. Your second channel is "creatively called" Nile Blue. I think you should create a NB logo with luminol just like you did in this video with NR.

@NileRed 8 жыл бұрын

+Melody Williams That is actually a pretty good idea!

@kulled Жыл бұрын

is this truly the comment that gave birth to the nile blue idea?

@UltimatelyEverything 4 жыл бұрын

I don't know how anyone can dislike Nile's videos they're great, interesting, educating and entertaining

@Hezarou 3 жыл бұрын

Someone who doesn't understand the chemistry and can't appreciate the effort he puts in.

@cadenbrown7407 3 жыл бұрын

@@Hezarou or they don’t like the editing style, lack of closed captioning etc. not everything is black and white and not everyone has to like everything you do

@Hezarou 3 жыл бұрын

@@cadenbrown7407 what? No, I'm not talking about appreciating the work he puts in; the effort. You can dislike the style, the captions, hell you can dislike Nile himself. But you can't deny he does what he loves and he puts effort in, I always appreciate people who put effort in what they like. So don't think everyone you meet online is as shallow as you believe they are.

@zodiakofficial4093 3 жыл бұрын

It could also be possible, that people accidently klick dislike. I also had it a few times, that I opened a video and I suddenly saw, that I disliked it before accidently without noticing🤔 I can't imagine, that anyone would dislike this!

@sissypissyrapper23 3 жыл бұрын

@@Hezarou You totally missed the point and reiterated a prior opinion. Excellent comprehension.

@ThePaintballgun 8 жыл бұрын

Why is there a random Australian dude that cuts in at 15:47? lol

@gamingstreams4703 5 жыл бұрын

Idk!

@FrenchFriesShallLive 5 жыл бұрын

LMAO

@daydreamer8344 5 жыл бұрын

I think it was just a voice taken from another video as he said 25 grams of what he was adding and then Nile repeated that.

@elephystry 5 жыл бұрын

Don't Even Bother IT’S EXTRACTIONS&IRE

@braedenh7421 4 жыл бұрын

ecstasy.apostasy ITS ALSO EXPLOSIONS & FIRE

@krap101 7 жыл бұрын

Clearly opaque... I see what you did there...

@NileRed 7 жыл бұрын

I am a wordsmith

@among-us-99999 6 жыл бұрын

krap101 i was about to comment this! 😭

@hamaljay 7 жыл бұрын

I like seeing your mistakes much more than successes. Thanks for keeping mistakes and sharing them with us.

@RazlGazl 5 жыл бұрын

Tasty mango smoothie Edit: Lemonade looks good too

@meepbeep2464 2 жыл бұрын

glowing gatorade

@omarbrooks6159 6 жыл бұрын

why couldnt my chemistry teacher make it this interesting i would've actually paid attention in class

@Riglockern 2 жыл бұрын

Cause they don't know what they talking bout

@zaybud6515 2 жыл бұрын

How much nilered has grown in the years is crazy, great job, keep it up

@Alex632 4 жыл бұрын

I'm still waiting on the future video of you exploring luminol. Its been long enough Nigel.

@ctbram0627 7 жыл бұрын

Isn't Hydrazine a fuel used in spacecraft reaction motors?

@samalbury9183 4 жыл бұрын

Yes

@Prototype5151 8 жыл бұрын

Fantastic results man!

@PatHaskell Жыл бұрын

I can’t tell you how refreshing it is to see a video presented by someone that knows what they’re talking about, is clear and concise without the unnecessary theatrics you see when people are trying to turn themselves into a social media personality and/or sell you something. I definitely would be open to more detail/instruction on the reaction mechanisms.

@samwarren2850 7 жыл бұрын

Thank you so much! I had to find an original experiment for college o-chem and this is so helpful!

@chasewilbur851 8 жыл бұрын

+Nile Red At 7:50 I don't think the ammonium carbonate annotation is supposed to be there.

@boxman139 5 жыл бұрын

I actually first learned about this chemical from playing ace attorney! So cool to see how it is made

@rileyawalt3381 2 жыл бұрын

same 😩

@RyannonBarrNeo 7 жыл бұрын

I want you as my chemistry teacher!

@wolftheshade7946 3 жыл бұрын

Luminol is so cool and interesting! Thank you for making these videos!

@Manofvideos292 2 жыл бұрын

It is 2:30 am December 2021. I've finished work, made the long drive home and I have chosen this video to watch. It's a 5 year old video at this point with 19 minutes of a pair of hands making a forensic liquid because I remembered how cool it looked on CSI.

@meiyuan2134 3 жыл бұрын

this is my favorite video of yours nile! i love forensics :)

@forevermrsanime 2 жыл бұрын

The color it has when it first glows is my favorite color ever. I absolutely love that color.

@ThePaintballgun 7 жыл бұрын

Followed this synth, one of the coolest things I've done.

@if860 3 жыл бұрын

I did this synthesis last week. From 200g of phtalic anhydride (pure p.a., Acros) i got 40 grams of 3-nitrophtalic acid. I took 10g of acid which yielded about 8g (6g after drying in oven) of luminol. All steps were identical, except I used pure sodium dithionite, cause it can be bought over the counter in Poland. Only last step was a bit different - I dumped the sodium dithionite to the solution, brought it to a short boil, and left it to cool. Great video.

@BrooklyKnight 7 жыл бұрын

That voice at 15:56 reminds me of "2 shots of vodka".

@Haemoglobuli 4 жыл бұрын

AND THERE YOU HAVE IT

@alexisflory6496 3 жыл бұрын

It's been 4 years. Did you forget about the luminol and blood experiment? I can't find it on Nile Blue either.

@flagmuffin1221 7 жыл бұрын

Ah, the old heating the test-tube with a heat gun trick. Had to do a diphenylacetylene and then hexaphenylbenzene synthesis recently which involved exactly that, except with little to no solvent.

@lookmedusa 3 жыл бұрын

Just want to let you know this is my favorite KZfaq channel.

@jonathanvanhyning3344 8 жыл бұрын

Brovo! Some of your best work. :) keep it up!

@C134B 2 жыл бұрын

I love that you're using pieces of old papers you printed, been there.

@Feetkiller97 8 жыл бұрын

love it once again

@Mrjmaxted0291 5 жыл бұрын

I've just discovered your channel and I'm completely blown away. This is proper hands on chemistry, a clear demonstration of various chemical reactions resulting in a variety of products using simple language that is accessible to anyone. In chemistry classes these days it feels like so much time is spent trying to get students to think in abstract terms about theory that they rarely if ever get to experience genuine practice. In my opinion this approach to learning is backwards; you shouldn't try to teach someone to do something before you do it, you should walk them through and SHOW them the process in action, and then seek to explain it in detail!

@redpractition 5 жыл бұрын

Mrjmaxted0291 yeah i completely agree

@divyanshugogna6152 4 жыл бұрын

I follwed this procedure and was able to get 1.33 grams of luminol in my chem class, thanks nile red. But the oxidation didn't work out for me cause of lab misinterpreting ferricyanide with ferrocyanide so had to use bleach for that. I loved this experiment.

@FennecTECH 7 жыл бұрын

11:01 looks like slightly off orange juce

@lorenzo42p 6 жыл бұрын

I'd drink it

@aperson4075 6 жыл бұрын

Pete Lorenzo i wouldn't

@eliasvila2984 6 жыл бұрын

sunnyD

@thisseagamer8165 6 жыл бұрын

The deadly orange juice...

@ht_sheehan 5 жыл бұрын

Mmmmm refreshing

@periwinkle43 Жыл бұрын

Got interested in the synthesis playing Trauma Team and thought to myself "NileRed should synthesize and test luminol" then it turns out he already did! What a treat

@contentlocked99 4 жыл бұрын

Vacuum filtration looks so satisfying.

@mrchangcooler 8 жыл бұрын

That heat gun seems super useful.

@messiasbapbaptista353 3 жыл бұрын

Excellent videos.

@bred_slice.69 5 жыл бұрын

When luminol glows in the dark it’s just so fascinating

@felixar90 6 жыл бұрын

That orange colour looks delicious

@PhilXavierSierraJones 6 жыл бұрын

I guess iron ion in Hemoglobin acts as catalyst when it comes into contact with hydrogen peroxide and Luminol mixture.

@nickwade1780 6 жыл бұрын

What other reducing agents do you think would work in the final reduction to make Luminol? Do you think sodium borohydride could reduce the nitro group?

@jhyland87 4 жыл бұрын

Either you're part Australian... Or you were watching an E&I video while editing this and didn't realize you included his voice.. lol

@TinyCutebug 4 жыл бұрын

These videos get me through my anxiety attacks

@maxpavlenko8172 8 жыл бұрын

Nice video!Thanks

@BeardedTate 2 жыл бұрын

Thanks!

@BeardedTate 2 жыл бұрын

Have a coffee

@IsabelHernandez-cm5bd 3 жыл бұрын

Doing a lab report without doing the exp so I’m glad I get to see what I could’ve done

@MinEntropy 8 жыл бұрын

Next up: Synthesis of fluoroantimonic acid! Please.

@MrMatnor2403 8 жыл бұрын

Ain't that the most corrosive acid in the world?

@Thyagohills 8 жыл бұрын

+MrMatnor2403 yup.

@shiningarmor2838 6 жыл бұрын

You're going to need a lot of teflon. Good luck.

@aakansh45yearsago83 4 жыл бұрын

Teflon: I'm ready baby!

@smokingsamosa 7 жыл бұрын

"it becomes clearly opaque"

@a3xccy379 8 жыл бұрын

Awesome video as always nile it was pleasure to hear your real voice !!!

@NileRed 8 жыл бұрын

+Rajas Godbole but I always use my real voice?

@lkexpress 8 жыл бұрын

+Nile Red He was referring to the voice that said "25 grams of sodium bicarbonate". Sounded Australian

@NileRed 8 жыл бұрын

Ludo Kressh haha it was my friend (who decided to put on an accent)

@a3xccy379 8 жыл бұрын

lol

@nicolasbug 8 жыл бұрын

Excellent !!!! How du you find enough time to do all go this...

@samuelalbritton2224 5 жыл бұрын

All I watch is forensic files and this is on every episode!!!!

@HighFidelityFox 7 жыл бұрын

15:00 heh I made a video with those exact ingredients yesterday. I didn't know you made one :P

@harrywoodman2988 6 жыл бұрын

Sodium dithionite is only metastable and rapidly decomposes in acid. My guess is the household version has the bicarb as a stabilizer. That's also why you get it as a possible range. Even the chem suppliers sell it as such and you usually use an excess. Again, nice hands NileRed. Mechanism is off, consider the first step going through the anhydride of the diacid (made by dehydration under the high heat). Second addition would also go through an anhydride or ester amide intermediate (from your glycerol or a dimer of the acids). Excellent demonstration.

@pietrotettamanti7239 6 жыл бұрын

Wait a minute. If the max amount of the other chemicals is 66,5% how can the sodium dithionite be present in only 15% concentration?

@macfixer01 6 жыл бұрын

Back in the early 1970's when I was in junior high school there was an (I'm guessing 1950's vintage?) chemistry experiments book in the school library that had a whole chapter on Chemiluminescence. It gave multiple formulas including this same one, and several others such as a deep red light made using Pyrogallol. As sources for the chemicals it listed Kodak, and also a Varniton Company located in Burbank California as I recall. Some other experiments shown were making pyrophoric lead, and dissolving cellulose then extruding Rayon. I'm just giving a couple examples from memory to see if maybe this rings a bell with anyone that may have the book or know the title and publisher info? I've always wanted to find a copy of this book, or to at least get a photocopy of it. Does anyone know of it? Thanks in advance.

@ripple9484 7 жыл бұрын

If you start at n2h to nh2 before the vacuum pump would you be able to fallow the rest of the step as is or would it turn out a bit thicker or unusable

@ClannerJake 7 жыл бұрын

hey Nile, I've been wondering- since glowsticks are a chemical reaction can you reverse it or I should say extract the luminal for reuse(if so how difficult would that be). thanks.

@megagatlingpea2322 Жыл бұрын

glowsticks dont use luminol or else why do they glow for more than an hour. we saw that it only lasts for about a minute so that's a stupid question

@bipolarchemist 5 жыл бұрын

Not that palladium on carbon is easy to come by, but in theory, you could have used hydrazine sulfate to reduce the nitro group as well, but as I've never run such reactions in glycerol, I can't say for certain if this could be converted to a 1 pot process.

@chamarystigers8731 4 жыл бұрын

Cool glow in dark acid

@daydreamer8344 5 жыл бұрын

I always call heat guns industrial hairdryers. It make all the wood shop jockeys angry.

@someonessidechannel1485 6 жыл бұрын

Question: Would you be able to change its color with it still remaining stable

@stonent 7 жыл бұрын

15:53 what? Random australian?

@Jamietron666 4 жыл бұрын

*new zealander

@krissp8712 4 жыл бұрын

Way too nasal to be a kiwi!

@ashmiashiq1855 Жыл бұрын

please make a video on stilbene derived optical brightening agents

@matlilly8795 5 жыл бұрын

I have read the masks which drop down for emergencies on a jet are a chemical reaction to produce oxygen. I would love to see a reaction which produces "potable" oxygen.

@kinetikx 6 жыл бұрын

I'm no chemist, but what I do know from other endeavors is that having your thermometer directly in contact with your beaker during heating might not be the best idea. It has to introduce some variance due to the thermal nature of the glass. I'd think you would want the business end of the thermometer in contact with nothing but the solution you're monitoring. I guess since there is a temp. range in this reaction it isn't that critical... But I've found that using best practices habitually ends up being better in the long run.

@billsmathers7787 8 жыл бұрын

Some quick questions about this synthesis: 1) Why did you use acetate to free the hydrazine? Wouldn't hydrazine (bp 114 C) boil off before the acid (bp 119 C) does? Chloride seems like it would accomplish the same goal, except it is even easier to obtain and has a lower boiling point. It also seems like the acetic acid and hydrazine might have potentially reacted to acetylhydrazide, hurting your yield. 2) Why was the nitro reduction done after the hydrazide was formed? The basic conditions are capable of hydrolysing hydrazides.

@porkchopSMIN 8 жыл бұрын

Finally here!

@inactive417 5 жыл бұрын

So I’m deciding to try to make luminol for my science project. *Boy, I don’t know what I’ve just done.*

@viteksimek2936 6 жыл бұрын

Hi, please, if it's possible, wouldn't you like to make a video about how to get an iron from the blood?

@pheo4156 5 жыл бұрын

If you wanted to do this reaction with pure hydrazine, what would change? could you just directly add the 3nitroacid thingy with hydrazine and have the rest of the reaction be the same?

@maricedillo9413 2 жыл бұрын

Omg I always wanted to do luminol in my school

@jaymeselliot8181 5 жыл бұрын

i never get tired of seeing things change color and get vacuum filtered 0-o

@giovannadallalba2126 2 жыл бұрын

I can't understand shit about chemestry but I still enjoy watching Nile's videos

@lubomirgallo1605 7 ай бұрын

@NileRed great interesting video, maybe i'll try it myself..... something else i wanted. You're using mainly magnetic stirer that means you don't have to have boiling stones but from my experience it's better to have boiling stones. Mainly for destilations and methods where is required lot of heat. My magnetic stir hotplate is not efficent as yours because of that i'm using old ETA spiral hotplate (slovakian thing) , it's capable of much higher temperatures (sometimes really crazy) as my magnetic stirer. Well in that case i need boiling stones. At school we're using broken porcelain plates but i personaly prefer ceramic filtration rolls from aquarium filter because they don't react and have much more surface than porcelain fragments. Thanks that i can use much higher temperatures without bobbling out.

@aakansh45yearsago83 4 жыл бұрын

After it stops glowing, is it like permanent or it'll start glowing again as we repour it to another beaker?

@Dakorian100 8 жыл бұрын

Finally, thank you!

@NileRed 8 жыл бұрын

+Dakorian100 no problem!

@JediBuddhist 6 жыл бұрын

God.. Beautiful craft. This one lost me atm.. Keep em comin Dude

@dannyassmann7331 3 жыл бұрын

"Clearly opaque" I like what you did there... 😁

@KnakuanaRka 5 жыл бұрын

Incidentally, would it be easier to purify the sodium dithionite from your rust cleaner before you put it into the reaction, rather than having to deal with the foaming and having to repurify the luminol to deal with the additives? Or is it fine this way? You’ve done plenty of purifying and extracting from household stuff before, including a video on how to do a more general procedure, so is there some difficulty in isolating dithionite, or is it simply more practical to purify it afterwards?

@jebug29 8 жыл бұрын

Woo! You finally made it! I've been wanting to make Zinc Sulfide to play around with phosphorescence. I tried it the other day and didn't have a black light handy to test it out, but I don't think it worked because it didn't pop like fireworks when it burned (and the product was black).

@NileRed 8 жыл бұрын

+Jesse Downing hmm, where did you get your reagents?

@jebug29 8 жыл бұрын

Nile Red I'll have to look and see where they originally came from. They've been sitting in our supply closet for years.

@NileRed 8 жыл бұрын

***** Yeah they might not be the most pure

@johnraimondi1811 4 жыл бұрын

In lab we always use these long wooden toothpicks to induce boiling

@pinksapphire2898 7 жыл бұрын

Question, where do you get your vacuum filter? i found a hand pumped one that costs like $60 and i really thought it was a rip off so i didn't buy it

@DragonisRed1 6 жыл бұрын

Is there any other way of reducing NO2 to NH2 by something else except of sodium dithionite? In my contry is not widely available :/

@dabackupplan3148 2 жыл бұрын

The forbidden snowcone topping

@SolarMillUSA Жыл бұрын

How do you clean your buchner funnels? Are you using a paper filter on top of the frit? It's hard to tell from the video.

@editname6868 5 жыл бұрын

12:51 bit it’s color is neutral color for your ph indicator (you made a ph indicator

@pencrows 5 жыл бұрын

8:07 nice smoothie

@RaExpIn 8 жыл бұрын

High res equations! Haha :) Nice video!

@SurajC_surc999 8 жыл бұрын

What is the source of hydrogen in the reduction of the nitro group to an amino group?

@evan2587 6 жыл бұрын

Hey can I use sodium dithionite to reduce nitrobenzene?

@paytonpryor 3 жыл бұрын

To me it looked like you turned chemicals in to Pepsi, into orangejuice, into egg drop soup, then butter, and ended up with a new mineral foundation called luminol.

@tsraikage 4 жыл бұрын

is there anything like thermoluminescent chemical that if you worm up compared to current temperature emits visible light but if you leave at this temperature slowly dims. if you cool down becomes black and cycle begins again...?

@akhilthechemist 4 жыл бұрын

Cant we use sodium sulfite or sodium thiosulfate in place of sodium dithionite

@periwinkle43 Жыл бұрын

Would there be any differences to using 5-nitrophthalhydrazide as opposed to the 3-nitrophthalhydrazide synthesized in thd video?

@firstlast-cs6eg 5 жыл бұрын

I understand why you filtered after the Iron out. But why did you filter before then? Wasn't all the materials before then, pure chemical ingredients?

@jdflyback 8 жыл бұрын

Buy a cheap ceramic coffee mug, break a few pieces off and crush until the right size, the porous ceramic makes for a good replacement for boiling stones.

@NileRed 8 жыл бұрын

+jdflyback That is very true. I am just lazy :p