MASTERING TISSUE CULTURE PLANTS | HOW TO ACCLIMATE PLANTS FROM TISSUE CULTURE

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MASTERING TISSUE CULTURE PLANTS | HOW TO ACCLIMATE PLANTS FROM TISSUE CULTURE

Рет қаралды 16,646

Күн бұрын

Acclimating tissue culture plants can be a challenge especially if it’s new to you. I’m showing every step that I take to have 100% success acclimating any plant from TC!
Acclimation steps:
www.parkfolia....
Supplies I use:
Amazon US www.amazon.com...
Amazon CA www.amazon.ca/...
Music:
Hiatus - MERK-E-BONGWTR
/ merk-e-bongwtr

Пікірлер: 78

@brookewarra9520 4 ай бұрын

I'm waiting on my very first TC and I'll be honest, I had no idea what I was getting into. I just happened to look up the term tc+prop tonight and seriously I have been through so many videos & felt so confused and overwhelmed until I got to your content. Very informative and straight forward and detailed info. You're a lifesaver. I can't wait for my new little dude to show up!

@Parkfolia 4 ай бұрын

I’m so glad this was helpful!! You got this 💪 it’s intimidating dealing with your first tc plants but once you get the hang of it, it’s a ton of fun watching teeny tiny plants grow into their full size

@Miles_Hoffman Жыл бұрын

Thank you, I love learning from others mistakes. Please make more content about how different plants need different care. 😊

@skepticalhippopotamom Жыл бұрын

thanks for sharing!! tbh i would love to see some content on multiplying plants too if you have some time :) !!

@Parkfolia Жыл бұрын

Of course!! I’m going to try and do some filming while I work on plants in the still air box soon 😄

@hberror404 11 ай бұрын

Yaaay more TC content on KZfaq!! 🎉🎉🎉🎉

@vivity01 Жыл бұрын

Just received my first tissue culture from Etsy and was totally lost. Thank you for your professional, informative video! Looking forward to some of your culture offerings. Well done!

@Parkfolia Жыл бұрын

Thank you very much! I appreciate it and glad it helped you 🥰 happy growing!!

@vivity01 Жыл бұрын

The only thing I was not clear on was, when using two plastic cups as a dome, did the bottom cup need drainage holes?

@Parkfolia Жыл бұрын

@@vivity01 nope! I keep a reservoir of water in the bottom with this mix so no drainage holes are necessary

@buffalo7480 Жыл бұрын

I haven't seen your content else where, but you've nailed this vid. I'd never heard of that media. It takes a little longer, but I would love to see a comparison of different methods or protocols if you do Tc yourself. Great job! Looking forward to more content

@Parkfolia Жыл бұрын

Thank you so much! I can definitely make some comparison videos, it would be an interesting experiment to see the growth pattern of different protocols

@vmota60 6 ай бұрын

Please do more videos! I am new to ptc and want to learn from experienced people.

@realpain84 11 ай бұрын

Great content, so much to learn for me, thanks for sharing ❤

@TheSkyHive 11 ай бұрын

Just found your channel and subbed! I have been growing fungi for 27 years and recently started a mushroom farm. I have many industrial size pieces of equipment like my autoclave and flow hood, I have ALWAYS wanted to get into tissue culturing and I think it will be my fall project. The Biocouplers intrigue me. Have you made a video about the pros and cons of using them versus older tried and true techs?

@ralfvk.4571 Жыл бұрын

That's such a cool thing, wow. Thank you very much for this professional Video and information. ❤

@davidmaisel8062 9 ай бұрын

It would be nice to have a video showing the multiplication step on a few species and why different mediums are used.

@alfredosantos6669 Ай бұрын

Good information. Thank you. Happy 4th of July.

@GrawRious 3 ай бұрын

I’m about to buy some banana plants that are tissue cultured and this was so helpful.

@sunshinecarnivores1919 7 ай бұрын

This is helpful. Most of the plants I want are carnivorous plants and the cheaper ones are in tissue culture.

@buckbeaksgarden761 8 ай бұрын

I hope you do more!

@elvisgonzalez3798 4 ай бұрын

Hey, thanks for the great content. Wondering if neem oil can be used as fungicide when cleaning the plant?

@prettyingreen 8 ай бұрын

Subbed!! I hope you do more videos 🙌

@EverythingPlants Жыл бұрын

Great instructional video and is this the substrate you send with the plants when purchased?

@Parkfolia Жыл бұрын

I never used to but I am moving to sending this mix instead! My holy grail used to be coco coir and perlite which is still a great simple substrate to use, but I’ve had much better success with stratum 😃

@DEAexotics 9 ай бұрын

great information

@Parkfolia 8 ай бұрын

Glad you liked it! :)

@MrDirkles Жыл бұрын

To avoid having contamination issues when using working with growth media for plants i have found that the following process is essential after 10 years working with GM plants. *wash your hands and lower arms thoroughly with soap ( most important step) * wipe down flow hood with 70% ethanol. * Before you put your containers, bottles, plates etc in the working area wipe them down with 70% ethanol. * spray a little 70% ethanol on your hands before you start. * Have a bottle of 70% ethanol in your working space ( This is kept specifically for flow hood) and decant some into a beaker to sterilize tools whilst working. * Have a flame ( away from the ethanol) so that every tool is dipped in the ethanol and then passed through the flame before the tool is used. * If working with plates , only raise the lid the minimum and never place the lids upside down in the flow hood.

@Parkfolia Жыл бұрын

Great tips thank you!! Have you ever used a 10% bleach mixture instead of ethanol when working?

@MrDirkles Жыл бұрын

@@Parkfolia I only use a bleach Solution for seed sterilisation. To save yourself a lot of headaches doing this stuff I'd recommend that people find a published protocol and follow it. For example, this paper (1985). A Simple and General Method for Transferring Genes into Plants. , 227(4691), 1229-1231. doi:10.1126/science.227.4691.1229 Is the most common plant cell culture transformation method used. You can download the full paper by visiting sci-hub This site allows to download any scientific paper even if it is behind a paywall.

@max420thc 10 ай бұрын

If you are working with any hemp, marijuana, cannabis plant this will not work. A virus has been spreading through growers crops called HLVd. Or hops latent viroid disease. Alcohol will not kill it and you will spread it through your utensil's. The only thing that kills it is bleach , I suggest keeping your surface area clean with bleach and your tools with bleach then wipe they areas down after using the bleach with alcohol , your plants will not like the household bleach at 20 percent ,

@max420thc 10 ай бұрын

I use a 20 percent bleach, new single edge razor blades for each plant , then replace it. Then after using bleach I clean the surface areas with alcohol after that. The plants will not like the 20 percent household bleach. Not dichlor, but household bleach.

@sousafamilygarden 5 ай бұрын

Question please - how to order the tissue culture kit to try it myself - thanks Great Video thanks so very much

@jamess1732 3 ай бұрын

Do they need bright light?

@muddypawswanderlust Жыл бұрын

Please helllp! 😭 I messed up! I accidentally added 30gr/L of agar instead of 6gr/L. I used an expensive DKW media and other expensive additives and hormones and I don't wanna waste it! Will this too high concentration of agar affects plant growth?

@Parkfolia Жыл бұрын

🥺 hate to break bad news, but I think so..I imagine the agar will be rock solid from 30g/L so I think it will be difficult for the plant to make good contact with the media. Keep me updated how it goes though, I am curious if the plants will still be able to absorb the nutrients with such a dense media!

@rosewest5168 3 ай бұрын

Hi Do you put the zip lock bag under a grow light please? Or is it best not too?

@tulsiramdagur9580 Жыл бұрын

हेलो दोस्त आप कैसे हैं। आपके द्वारा टीशु कल्चर पर बहुत सुंदर जानकारी देते हैं। आप भी बहुत सुंदर हो आपके टेटु भी बहुत मस्त है

@gettinluckyfishin4514 7 ай бұрын

@Parkfolia, great info! By the way, what brand and model lights are you using on the racks behind you?

@Parkfolia 7 ай бұрын

They’re Barrina lights! I believe the LED T8 version

@natachapetite1008 Жыл бұрын

Is it good to leave them on a heatmat ?

@Parkfolia Жыл бұрын

I prefer without a heat mat, it can get REALLY warm in the sealed environment and speed up potential mold growth or melt the plant from the heat in my experience

@Weightlosspendotuk 10 ай бұрын

Hi u ever thought about using ozone to sterilize

@Sergiu.93 2 ай бұрын

What do you use for CT? ms + naa and bap?

@geronimomiles312 Жыл бұрын

What would I do with Aquarium plants , sold as tissue culture , that I want submerged? Supposedly it's routine for them to melt.

@Parkfolia Жыл бұрын

I have a few TC plants in my aquarium, they can be a bit harder to establish, i probably lost 1/4 of my dwarf hairgrass when I planted it out so I’m definitely not an expert on the aquatic side of things! The biggest tip I can give is make sure 100% of the gel is rinsed off before planting them in your tank and give it a dose of fertilizer to start growing roots right away

@user-xz1rt2fk6c 5 ай бұрын

How about using lechuza pon?

@shellym79 Жыл бұрын

Could you use just perlite? A bag of fluval is literaly the same price I paid for my TC plant.

@Parkfolia 11 ай бұрын

Yes absolutely! I like adding fluval because it releases nutrients but straight perlite with a small reservoir works great as well

@ericalvarez889 11 ай бұрын

By using two cups with tape aren't you risking contamination? Or am I overthinking

@Parkfolia 11 ай бұрын

Keeping a sterile environment isn’t much of a concern anymore once they’re planted, the idea is to keep high humidity and with the cups taped together it keeps the environment sealed at 100% humidity :)

@ericalvarez889 10 ай бұрын

@@Parkfolia thank you for explaining! 😊 sorry totally new to all this.

@robertl6711 2 ай бұрын

Can I use RO water?

@svcruz 9 ай бұрын

I notice your distilled water is PC brand, if your in Canada, where do you buy TC plants to ship into Canada?

@Parkfolia 8 ай бұрын

TC 101 group is where I've found a few sellers! I don't really buy them anymore, there's one on Etsy as well who I used when I first started and they were great, I think the name of the shop is Plantsme or Plantsme2 something along the lines of that

@CHIROTHECA 4 ай бұрын

0.3-0.5% Peroxide!

@rickprater7159 11 ай бұрын

Wonder if I could add very diluted superthrive?

@dillonchamberlain 10 ай бұрын

What for?

@user-jj9zr4rv5x 9 ай бұрын

Where do you get those little jars from??

@Parkfolia 8 ай бұрын

A lot I've kept from past TC orders or purchased from other local sellers who had unused jars! I have found them on Aliexpress too though and they're kind of expensive on there but if you don't need a lot it works great. I believe ULINE also has a few sized jars with polypropylene lids but I haven't tried those yet

@noprialdi5032 11 ай бұрын

nice vidio

@Sama_09 8 ай бұрын

Where do you recommend to buy the tissue cultured plants ?

@somerb33 8 ай бұрын

I just bought some from Orange Lake Nursery!

@Sama_09 6 ай бұрын

nice @@somerb33

@gardeningonly 7 ай бұрын

Do you use flow Hood?

@Parkfolia 6 ай бұрын

I don’t! I’ll be making a video soon on the still air box that I use ☺️

@gardeningonly 6 ай бұрын

@@Parkfolia thank you

@charliemagoo7943 11 ай бұрын

It seems like all the tc videos I see are generic. Mix this stuff up. Throw a piece of plant in there. 2 weeks later you have 1 million plants in a test tube. Hmmm. Ok in a test tube what is considered a plant? 1 root. 1 cell. ??? How to divide the million plants in the test tube without injuring or turning them into a combobulated mess.etc

@Parkfolia 11 ай бұрын

I really Appreciate this, honestly since doing it for a few years I tend to forget what is and isn’t a sort of “common knowledge” and I can absolutely make a video breaking down each step even further and explaining terminology

@charliemagoo7943 11 ай бұрын

@@Parkfolia will be waiting...I'm sure it'll be a video and not a short

@Lovely_planet 7 ай бұрын

@@charliemagoo7943sheeeeeesh

@tulsiramdagur9580 5 ай бұрын

Hello friends how are you. I needed blue berry plant.ms midiya.

@thawiseninja1559 3 ай бұрын

@@Parkfolialink that video here