Glad to hear that 🙌

Aww thank you!!! I tried something a little different this week -- glad to hear you enjoyed :)

I'm so with you on this, Chad! TC is what really tickles my brain in the plant world. I find it fascinating! I think there are tons of protocols that are good starting points available online. In my four years of experience playing with TC, I've found each species, and potentially sub-species, needs its own curated recipe for that EXACT plant. This can be tough because I usually make 1 Liter of Multiplication media at a time, and that fills 20-30 vessels. So I usually just put Monstera, Philo, Epi, all in the generic Multiplication Media I've made for that day. But I would say I change the mix almost every time I make it!

@@prettyingreen Thanks for the tips! I'm not jumping into tissue culture just yet, but if I do I may want to experiment with a Pink Pineapple, or other patented plants like the Philo Gabby.

I can understand your concerns.. I am trained for TC, I work in the biotechnology field, and yes protocols are well guarded because it's intellectual property. But if you understand how it works an learn the basics, it's not that complicated :) Personnaly I prefer not to do this at home because the quality of the materials and equipements can cause issues... and of course knowing how to manipulate under a BSC requires some training. I think it's best to leave this kind of stuff to people that really know what they are doing and have real lab conditions 😅

@@aroidaddiction I have Biotechnology diploma 🙂 bio tech is a very wide field and used for much things TC is biotechnology !

@@alienperfection973 i'll do what i want thanks, easy to find studies with protocols

You can make an environment sterile enough by using a still air box, I'm sure his flow hood is fine :D

Undifferentiated cells AKA stem cells, exists in the plants structure, like the stem. "Cuttings" typically have one node from which one plant can grow out from. But with the activation of stem cells, multiple plants can grow from one stem. The protocols (aka the growth medium mix) can activate stem cells to grow whatever we want. The initial phase are leaves (to create new clones) second phase is the activation of the roots.

@@ChadGardenSinLA thank you! I think I wasn't realizing that the cutting wasn't only growing normally from the node in a perfect environment but also multiplying quickly due to the stem cells. This video and your comment explained all of this much better than other videos I have watched about it.

This is so interesting. It’s not easy, but hopefully more TC will enable me to get a Thai less expensive. Thanks so much for your work on TC & for sharing!

This is exactly the beauty and speed TC provides over typical stem propagation!

@@prettyingreenOK....ive come back to this comment because I was looking for someone who knows about tissue culture and can maybe help me with a plant problem I'm having. Basically, I had an apparently healthy plant that rotted quickly and I'm wondering if it could be related to TC. I'llstry to keep it short but give the important details. About a month ago I ordered an N'joy pothos. I got it the next week and it looked beautiful. I let it just sit for a couple days but then repotted it because it was just in a soggy coir mix. The roots looked good....white, maybe a bit thin, but healthy for sure. Each individual plant (I think there were 6) were all crowded in the very center of the pot and there were a LOT of little sprouts, too small to identify as pothos or not. I separated, repotted, and after about a week I noticed they weren't perking up the way I expected. I tugged on the plants and almost ALL of them just came right out and were black and rotted under the soil. Could this possibly be because it wasn't hardened off after TC? And could those sprouts have been because it was still a bit exposed to the hormones and making babies? I don't think it could be the soil because I potted the pothos with another plant and it is just as happy as ever. It also couldn't be over watering. If it were pests or already rotted, I would have seen the evidence when removing the old soil....thanks so much if you see this LONG comment! I'm just trying to figure it out and I'm hoping I don't need to worry about my adansonii (the plant it was potted with) . Could it be because it wasn't hardened off properly? Also, if it's important I got it on amazon from California tropicals.

Glad you enjoyed it! TC is really fun and challenging :)

Absolutely! 😄

I think it's very much possible to create an "affordable" tissue lab for

@@prettyingreen I have a lot of questions. 😆 Thank you for the informative videos!

🤗

Absolutely! I enjoyed making this video 😄 More like it to come!

It is! Big fan of TC

Agar agar? I think it's a type of gelatin like seaweed

Same question here

It’s agar agar ​@@queeniefelixjoiedomantay1913

@@beanerb822yes but with plant food and other hormones. This process would be hard to replicate in a home due to the lack of a sterile environment.

Aeroplane

So glad you enjoyed!! 😄

Ahhhh thank you Rose!!

Correct. I guess once it’s severed from the mother plant, it becomes an “explant”

Correct! I was using a Golden Pothos in the video bc it likely will have contamination due to me talking through the entire video without the laminar flow hood on, and handling it incorrectly

Thank you!!!

Can be a long time if one is proving their clones as stable, healthy and hardy for long life in normal conditions before distributing to the public. Short answer is maybe 3 months from plant cutting to sell question mark plants in dirt with minimal acclimatization.

I’ve found TC doesn’t necessarily breed weak plants. It can at times due to somalclonal variation from repeated sub culturing, but that can also result in positive benefits too.

TC makes plants more vigorous, at least temporarily.

Absolutely wear mask. Gloves are dependent on style of technician

True!

Lights are usually 16H on. Temps around 76f

Yessssss 😄🤗

😄

Move to a country where labor is basically worthless and apply for the job.

You can make another plant out of ANY cell of a plant. Even a single cell. It's just harder.

It's MY laminar flow hood: en.wikipedia.org/wiki/Laminar_flow

What he's saying is, your filter is not producing a laminar flow of air (which it's not), which is the sole purpose of a "laminar flow" hood

It's not laminar flow. Also, you shouldn't use a magnetic stirrer for sterilizing explants, the magnet can damage cells and cause somatic mutations.@@prettyingreen

Lol