I'm A/l agri student so this video is a very important for me.And i like this video .thank u so much❤❤
@prettyingreen5 ай бұрын
Glad to hear that 🙌
@northernplantuckyplantcinc2630 Жыл бұрын
I think this may be my favorite video you've done so far❤🎉
@prettyingreen Жыл бұрын
Aww thank you!!! I tried something a little different this week -- glad to hear you enjoyed :)
@ChadGardenSinLA Жыл бұрын
I can't express enough how much into the science I am with this hobby! Thank you for making this video. I'm curious to know if the protocols you used are generally available online for "common" plants like pothos. I understand why they are a well guarded secret, but I've seen a lot of small nurseries here in California (as well as instagrammers) performing tissue culture in their back rooms. So it makes me wonder how readily available (or shared) certain protocols are seeing so many hoods outside of a hospital or lab setting.
@prettyingreen Жыл бұрын
I'm so with you on this, Chad! TC is what really tickles my brain in the plant world. I find it fascinating! I think there are tons of protocols that are good starting points available online. In my four years of experience playing with TC, I've found each species, and potentially sub-species, needs its own curated recipe for that EXACT plant. This can be tough because I usually make 1 Liter of Multiplication media at a time, and that fills 20-30 vessels. So I usually just put Monstera, Philo, Epi, all in the generic Multiplication Media I've made for that day. But I would say I change the mix almost every time I make it!
@ChadGardenSinLA Жыл бұрын
@@prettyingreen Thanks for the tips! I'm not jumping into tissue culture just yet, but if I do I may want to experiment with a Pink Pineapple, or other patented plants like the Philo Gabby.
@alienperfection973 Жыл бұрын
I can understand your concerns.. I am trained for TC, I work in the biotechnology field, and yes protocols are well guarded because it's intellectual property. But if you understand how it works an learn the basics, it's not that complicated :) Personnaly I prefer not to do this at home because the quality of the materials and equipements can cause issues... and of course knowing how to manipulate under a BSC requires some training. I think it's best to leave this kind of stuff to people that really know what they are doing and have real lab conditions 😅
@alienperfection973 Жыл бұрын
@@aroidaddiction I have Biotechnology diploma 🙂 bio tech is a very wide field and used for much things TC is biotechnology !
@Encrypt-8 ай бұрын
@@alienperfection973 i'll do what i want thanks, easy to find studies with protocols
@Eric-yj5xg13 күн бұрын
Check your flowhood air vortex. Sometimes, the way you mive your air around can create pockets of suction bringing bad air in the flowhokd.
@user-xv2sr5jo4l Жыл бұрын
I'm a pharmacy technician by trade and I specifically work in a sterile admixtures lab where I utilize aseptic technique to prepare drugs for intravenous use. I find the process of tissue culturing really fascinating and I kind of want to try it myself since it seems to fall in my lane. I'm a little curious about your setup, though, if you don't mind some questions. In a typical laminar flow hood, the air flow is in sheets (hence "laminar flow") from a singular direction. I could be totally wrong, but I don't think that can be achieved with the type of filter you're using. We also utilize PPE including (but definitely not limited to) sterile gloves to avoid introducing potential pathogens. I know I'm working with more sensitive mixtures, considering their administration, but do these practices not impact your process? I'm legitimately curious because I'd like to see if this is something I could set up at home and while a fully sterile environment would be impractical, I could probably rig something up if it doesn't need to be 100%!
@chitsu13889 ай бұрын
You can make an environment sterile enough by using a still air box, I'm sure his flow hood is fine :D
@lindsey4178 Жыл бұрын
could someone answer a question for me? I understand the concept of tissue culture BUT how is it any different from propagation aside from the sterile environment and the more nutrient rich growth medium. I just wonder, if the cutting and everything is all the same, is the only difference a more controlled environment? This may be a stupid question. Lol. I'm just trying to understand exactly how it works and why it's preferred to regular propagation? Especially with plants that aren't too difficult to propagate to begin with. EDIT....maybe I should have finished the video first. Lol. I think I understand a bit better. This was a much more in depth explaination than other videos I've seen. Very interesting
@ChadGardenSinLA Жыл бұрын
Undifferentiated cells AKA stem cells, exists in the plants structure, like the stem. "Cuttings" typically have one node from which one plant can grow out from. But with the activation of stem cells, multiple plants can grow from one stem. The protocols (aka the growth medium mix) can activate stem cells to grow whatever we want. The initial phase are leaves (to create new clones) second phase is the activation of the roots.
@lindsey4178 Жыл бұрын
@@ChadGardenSinLA thank you! I think I wasn't realizing that the cutting wasn't only growing normally from the node in a perfect environment but also multiplying quickly due to the stem cells. This video and your comment explained all of this much better than other videos I have watched about it.
@carolstuff Жыл бұрын
This is so interesting. It’s not easy, but hopefully more TC will enable me to get a Thai less expensive. Thanks so much for your work on TC & for sharing!
@prettyingreen Жыл бұрын
This is exactly the beauty and speed TC provides over typical stem propagation!
@lindsey4178 Жыл бұрын
@@prettyingreenOK....ive come back to this comment because I was looking for someone who knows about tissue culture and can maybe help me with a plant problem I'm having. Basically, I had an apparently healthy plant that rotted quickly and I'm wondering if it could be related to TC. I'llstry to keep it short but give the important details. About a month ago I ordered an N'joy pothos. I got it the next week and it looked beautiful. I let it just sit for a couple days but then repotted it because it was just in a soggy coir mix. The roots looked good....white, maybe a bit thin, but healthy for sure. Each individual plant (I think there were 6) were all crowded in the very center of the pot and there were a LOT of little sprouts, too small to identify as pothos or not. I separated, repotted, and after about a week I noticed they weren't perking up the way I expected. I tugged on the plants and almost ALL of them just came right out and were black and rotted under the soil. Could this possibly be because it wasn't hardened off after TC? And could those sprouts have been because it was still a bit exposed to the hormones and making babies? I don't think it could be the soil because I potted the pothos with another plant and it is just as happy as ever. It also couldn't be over watering. If it were pests or already rotted, I would have seen the evidence when removing the old soil....thanks so much if you see this LONG comment! I'm just trying to figure it out and I'm hoping I don't need to worry about my adansonii (the plant it was potted with) . Could it be because it wasn't hardened off properly? Also, if it's important I got it on amazon from California tropicals.
@THOTHvii Жыл бұрын
Wow, so I have watched about 8 videos pertaining to this subject and I wish I would have just started with this one. This is a very well put together video. I really like your brevity and concise instruction. I think TC is going to be my new hobby. Thanks for sharing the video.
@prettyingreen Жыл бұрын
Glad you enjoyed it! TC is really fun and challenging :)
@Lisa-gs7wj8 ай бұрын
It's nice to see how you explain everything about TC. I myself working on Plant tissue culture where I have to induce callus. I have used reported PGR for callusing but it seems like any hormone and its concentration has no effect on my leaf explant which is concerning. Also I'm using both young and mature leaves. What could be a reason for my leaf explant to not give any response on any callus inducing PGR? If you could answer my query?
@alimartinez1298 Жыл бұрын
This is fascinating! Thank you for the information!!
@karenfreedman9180 Жыл бұрын
I’ve always wondered… Thanks for sharing!❤️
@prettyingreen Жыл бұрын
Absolutely! 😄
@Iliketosaynish4 күн бұрын
Should mention if tissue culture plants or not by all sellers. Dr. Hussain
@plantypittsburgh Жыл бұрын
I am really looking forward to the future tissue culture videos. I harbor delusions of my own little lab some day. Another video topic that you might want to cover is light & variagation. I see conflicting advice and in my experience when increasing light should improve variagation, it does not. (I'm looking at you, cebu blue ghost). I've never had a plant revert. How does variagation happen? How is it replicated in tc and when can it not be? So many questions!
@prettyingreen Жыл бұрын
I think it's very much possible to create an "affordable" tissue lab for
@plantypittsburgh Жыл бұрын
@@prettyingreen I have a lot of questions. 😆 Thank you for the informative videos!
@Mario23558 Жыл бұрын
Very informative .🎉
@prettyingreen Жыл бұрын
🤗
@renatecormick7399 Жыл бұрын
Amazing, thank you so much for sharing this information, I enjoy learning about TC and the future of using it.
@prettyingreen Жыл бұрын
Absolutely! I enjoyed making this video 😄 More like it to come!
@rj69406 ай бұрын
Thank you for sharing the knowledge. Blessings to you 🙏🏻
@KimSol90 Жыл бұрын
This is truly incredible indeed.
@prettyingreen Жыл бұрын
It is! Big fan of TC
@user-bc1fx4qu1q Жыл бұрын
What kind of jelly did you use inside the bottle
@beanerb822 Жыл бұрын
Agar agar? I think it's a type of gelatin like seaweed
@queeniefelixjoiedomantay1913 Жыл бұрын
Same question here
@mahadihasanrahul98485 ай бұрын
It’s agar agar @@queeniefelixjoiedomantay1913
@amberfrazier5754 ай бұрын
@@beanerb822yes but with plant food and other hormones. This process would be hard to replicate in a home due to the lack of a sterile environment.
@geoffgeoff14312 күн бұрын
Aeroplane
@donnawest-newman956 Жыл бұрын
This is great information, thanks for dumbing this down. So interesting
@prettyingreen Жыл бұрын
So glad you enjoyed!! 😄
@rosemontez3674 Жыл бұрын
Thank you for sharing! What an amazing concept. Good job on this video and detailed explanation!
@prettyingreen Жыл бұрын
Ahhhh thank you Rose!!
@queeniefelixjoiedomantay1913 Жыл бұрын
Hello . I love your videos. Im really interested to learn more about tissue culture. All the tools and compounds needed. I really want to try it at home.
@BikramchaudharyB11 ай бұрын
When we select part of plant for tissue culture it's called explant not mother plant
@prettyingreen11 ай бұрын
Correct. I guess once it’s severed from the mother plant, it becomes an “explant”
@manjuk1708 Жыл бұрын
I think economically it makes sense to use tc for producing more monstera albo or Thai plants rather than golden pothos.
@prettyingreen Жыл бұрын
Correct! I was using a Golden Pothos in the video bc it likely will have contamination due to me talking through the entire video without the laminar flow hood on, and handling it incorrectly
@fikriefisal20428 сағат бұрын
Do you guys doing raspberry tc? Would the formula same as any plants that i can refer to?
@idealdrugs4 ай бұрын
Superb presentation !
@prettyingreen4 ай бұрын
Thank you!!!
@nelsonmayor93007 ай бұрын
excellent video, thanks for sharing. What antioxidant do you use for monstera? I have had serious problems with oxidation. Thanks
@jamiedelgado1484 Жыл бұрын
What a great video 👍
@user-lf7gl8ob8u5 ай бұрын
That is not producing laminar flow my good sir other then that great video
@khalidsultan7641 Жыл бұрын
A beautiful and wonderful channel, but I have a question. How long does plant tissue culture take until the seedlings are ready for sale? Thank you
@markeastridge9649 Жыл бұрын
Can be a long time if one is proving their clones as stable, healthy and hardy for long life in normal conditions before distributing to the public. Short answer is maybe 3 months from plant cutting to sell question mark plants in dirt with minimal acclimatization.
@greenhut16423 ай бұрын
Possible to helpe in tissue culture, my medium get contaminated every time
@bikexploration8 ай бұрын
The only reason I Don't do TC is of weak plants. It's more like quantity vs quality. And I'm more into quality.
@prettyingreen8 ай бұрын
I’ve found TC doesn’t necessarily breed weak plants. It can at times due to somalclonal variation from repeated sub culturing, but that can also result in positive benefits too.
@LarsLarsen776 ай бұрын
TC makes plants more vigorous, at least temporarily.
@greenhut1642 Жыл бұрын
Is it possible to make plant media at home, what ingredients are in growing medium. Possible to assist me sir
@miciglaric Жыл бұрын
Is plant also cleaned from viruses and diseases which are inside plant and does't allow plant to give full potential? Does tissue cultured plant have bigger yield?
@saraanderson4029 Жыл бұрын
When in the process do you usually transfer your tissue cultures into semi-hydro environment vrs soil?
@jack_irl4 ай бұрын
would you not wear a mask and gloves to avoid breathing on the TC?
@prettyingreen4 ай бұрын
Absolutely wear mask. Gloves are dependent on style of technician
@celgenbiotech5 ай бұрын
Do tou sell culture bottle of Albo variegated
@Strong_Tomato_5 ай бұрын
i think calling it the future of houseplants is a bit misleading because its been around for quite a while now(like 30+ years i think), nice video tho
@prettyingreen5 ай бұрын
True!
@mohan96706 ай бұрын
Is that possible to do tissue culture on elephant foot yam?
@greenhut16425 ай бұрын
How many hours light and AC must be on for tissue culture plants
@prettyingreen5 ай бұрын
Lights are usually 16H on. Temps around 76f
@jacobhicks7319 Жыл бұрын
6:14 those roots are JUICY
@prettyingreen Жыл бұрын
Yessssss 😄🤗
@pennymayo2332 Жыл бұрын
Interesting
@prettyingreen Жыл бұрын
😄
@madisontaff7379 Жыл бұрын
How do I get into this for a possible future job?
@LarsLarsen776 ай бұрын
Move to a country where labor is basically worthless and apply for the job.
@wazif4464 Жыл бұрын
wow very nice video👍 from my fren farhan safuddin
@jackfang58 Жыл бұрын
What is your sterile solution? I'm trying to replicate
@notgojo491 Жыл бұрын
so, basically the ,,you can make a new plant from every part of the plant, even from a piece of leaf" part is bs and you literaly propagate the plant by node, just on a fancy way
@LarsLarsen776 ай бұрын
You can make another plant out of ANY cell of a plant. Even a single cell. It's just harder.
@LarsLarsen77 Жыл бұрын
That's not a laminar flow hood.
@prettyingreen Жыл бұрын
It's MY laminar flow hood: en.wikipedia.org/wiki/Laminar_flow
@jordough4495 Жыл бұрын
What he's saying is, your filter is not producing a laminar flow of air (which it's not), which is the sole purpose of a "laminar flow" hood
@LarsLarsen779 ай бұрын
It's not laminar flow. Also, you shouldn't use a magnetic stirrer for sterilizing explants, the magnet can damage cells and cause somatic mutations.@@prettyingreen
@chatryna Жыл бұрын
Take away: don't build a house with a tissue cultured tree.
@prettyingreen Жыл бұрын
Lol
@420.........8 ай бұрын
1:45 You violated the area, you should never put your arms above the steril area.
@Subcribe_for_Subscribe5 ай бұрын
hi, can you make a tissue culture from a dried rose?