watching this in 2023 with DADA2 and ASVs lol

This series of video is better than most counterparts on coursera. Not to mention that coursera is no longer free ...

I just started studying microbiome and am trying to analyze the sequencing data. It's was helpful. THANKS A LOT!

great series!

So explanatory. Thank you.

Hi Dr Knights, thank you for providing this useful lecture. I am just wondering how I can find the fig. from the EMP, telling the percentages that do not hit the database vs. different habitats. I searched the EMP website, but it seems this fig. is buried deeply. Thank you!

Thanks. Your videos are really helpful.

Nice Dan-o! And good to see you on the Tube! At

Thank you sir very much

How can we analyse the metagenomic sequences that we get from company??

Thank you.

thanks a lot！！！

May I ask is there any difference between CD-HIT and Sumaclust? They both abandoned k-mer approach.

how to explain otu (NA:Not Available) of sequence reads. what does it means? are they exist?

May I ask, what clustering method would you prefer? And does the clustering method greatly affect the OTU richness @ abundance? Thank you

what about vsearch? its opensource and doing better than usearch as they said.

Rob knights son

Can u plz communicate us ur email ?