Have this system in my new home and this video helped a lot for the first replacement, especially for the inline carbon filter which hadn't been replaced since 2015. Thank you!

Microline says to leave the canisters loose and as water start coming out of the top of the prefilter, tighten it, then the same for middle/membrane, and after the prefilter and membrane are full, then open the water feed valve all the way and after a few to several minutes it should start coming out of the post/carbon filter and then tighten that last canister. Leave the RO tank closed and keep the faucet open and let water drip out for 2 hours, then open the RO tank valve and drain it completely. Once the RO tank is drained, then shut the faucet to completely refill the RO tank and the system will be ready after another approx. 2 hours for drinking. If you have the RO hooked to your fridge water dispenser, run a few large cups of water to get any carbon or sediment out and then everything should be good to go. Obviously pay attention to your fridge water filter too. I have my water dispenser running off the RO and the icemaker is separated and only runs off the fridge water filter. Hope this info helps.

Thank you for this video! We have just had this system installed and the manual says we need to sanitize the system/filters before using. Do you have any insight into this?

The grease that you put around the ring, would it be contaminated the water?