Microscopy: Super-Resolution Microscopy (Xiaowei Zhuang)

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Күн бұрын

Learn more: www.ibiology.org/talks/super-...
This lecture surveys a variety of recent methods that achieve higher resolution than is possible with conventional microscopy with diffraction-limited optics. These include different types of patterned illumination (e.g. STED and SIM microscopy) or techniques that build up an image by stochastically switching on single fluorescent molecules and localizing each molecule with high spatial precision (STORM, PALM, FPALM).

Пікірлер: 22

@CosmicEpiphany 9 жыл бұрын

Wow what a beautiful lecture! You really put a lot of time and effort into this presentation and this should be used as a guide for lecturers everywhere no matter what subject. I wish all teachers would put this much effort into conveying complex subjects.

@jinpan9905 Жыл бұрын

Recently, I am enthusiastic about the micro world invisible to our naked eye. I search for microscopies in optical or electronic. Unconsciously, I saw this tutorial, which impressed me a lot with the clear presentation. Finally, I find the young speaker is the famous Prof. Xiaowei Zhuang from USTC Special Class! What a small world.

@amossberg Жыл бұрын

That was great! I'm unlikely to need/use these advanced techniques but seeing them in action and presented so clearly is delightful and illuminating. I especially appreciate the shoutout to all of the researchers that contributed to these techniques at the end.

@anonviewerciv 3 жыл бұрын

Come with me, and you'll see A world of higher resolution. (6:26) 8:38 STED, RESOLFT. 11:11 SIM. 16:00 STORM, PALM, FPALM.

@j121212100 9 жыл бұрын

fascinating work! eye opening.

@11-1sp8 9 жыл бұрын

It's really a Pity that you did't get nobel prize this year.

@cuiqingli2077 9 жыл бұрын

I agree

@sigmalove1187 4 жыл бұрын

真正的顶级人才

@AB-rf1qf 6 жыл бұрын

This video is missing on the iBiology website!!!

@hawaiiguykailua6928 9 жыл бұрын

Hi Xiaowei, can you let us know if you have read or studied the optical system built by Royal Rife in the 1920's and his research and application of dividing light on live cells/virus at +60,000x's optical. As I understand it his microscope is still the most powerful scope for live cells, which allowed him and his team to research effects of tuned wave vibrations on cell walls. Ultimately building a verifiable wave table guide to destroy cell specific walls such as found in tumors, and other diseases. The research seems to have been thoroughly forgotten even though the team is the only one to have a 100% cure rate for terminally I'll patients with stage 3 or greater diagnoses before being treated under his and the USC medical chiefs care.

@rickynave 6 жыл бұрын

Hawaiiguy Kailua According to wikipedia: "Rife's claims could not be independently replicated, and were discredited by independent researchers during the 1950s". Science describes how our world works. If a scientific claim can't be replicated, it never existed.

@zapperman5344 4 жыл бұрын

@@rickynave The Rife microscope was exceedingly complex, actually using some of these techniques and others as well. Being able to replicate such work requires the ability to understand the principles involved and having the ability to implement them. Just because the person who tried to replicate failed does not mean that the results were not achievable by someone with greater understanding and insight. The main problem is that much of the work done by R.R. Rife was destroyed or just lost.

@sebastian5671 Жыл бұрын

@@zapperman5344 it didnt pass the scientific method, cry about it lol

@zapperdave9166 Жыл бұрын

@@sebastian5671 Show me the study where you found this.

@ErichAmMeer 2 жыл бұрын

厉害

@AshrafulIslam-pp3wl 4 жыл бұрын

I understood nicely and the pronunciation was imaging. I would like to attend another lecture(youtube). I think I could utilize this in vaccine and medicine molecule for HIV. Hope I will get help from you as well as Harvard University. THANKS.