Genetic screening & Genetic counselling. A-level Biology. Use of DNA probes & DNA hybridisation.

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This video covers the A-level theory of using labelled DNA probes and DNA hybridisation to locate specific alleles of genes.
These DNA probes can be used to screen patients for heritable diseases and for use in personalised medicine.
Discover the role of a genetic counsellor.
For past paper questions linked to this topic click these links.
missestruch.co.uk/
Timecodes:
0:00 Introduction
0:45 DNA probes
1:34 DNA hybridisation
2.37 Locating alleles of genes
4.29 Genetic screening
5.10 Personalised medicines
6.46 Genetic counselling
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Пікірлер: 25

@miaxx3929 2 жыл бұрын

This is very random but I really appreciated how calmly you spoke in this video, it really helped me concentrate and stay focused as I find this topic quite difficult 😊thank you

@MissEstruchBiology 2 жыл бұрын

ahh thank you. Glad my voice made it easy to listen to

@cocomuffin5007 4 ай бұрын

thank you so much for the whole topic 8 playlist - i feel like i finally understand!!

@MissEstruchBiology 3 ай бұрын

Glad it was helpful!

@kristinastoianova4870 3 жыл бұрын

very helpful thankyou!!!

@MissEstruchBiology 3 жыл бұрын

So pleased it helped you 😊

@uwotm8770 2 жыл бұрын

hi miss, do you have a video on gene therapy? Thank you so much

@zoegarnett9601 2 жыл бұрын

Hello, great video! I was just wondering, wouldn't washing away the DNA probes also wash away the hybridised DNA probe and patient DNA sample? Or is it like the ELISA test where the patients DNA sample is stuck to a surface and can't be washed away?

@brijrajbhugaloo 2 жыл бұрын

I'm guessing it's like the ELISA test

@zarazaraaa4753 2 жыл бұрын

would we asked to know how you find the dna base sequence? or could i simply say find the amino acid sequence-->mrna-->dna

@paolachoufani6532 2 жыл бұрын

do you have a video on DNA sequencing techniques? what is the Sanger method?

@qasimkhan34 4 ай бұрын

nice pfp. i can see ur filled with thoughts

@markdean6174 3 жыл бұрын

Thank you. Is this on the aqa spec?

@MissEstruchBiology 3 жыл бұрын

hello, yes, it's in topic 8.

@Tristilax 3 ай бұрын

Do you need to know the Sanger sequencing method?

@sxfaa.1 3 ай бұрын

Not for AQA

@maria-ht3cg 3 ай бұрын

Do we not use gel Electrophoresis in genetic screening? And what is the purpose of gel Electrophoresis?

@user-rd6ib8sk6r Ай бұрын

its used to separate the DNA fragments, so they can be heated to form single strands and allow DNA probes to bind

@saniyasiddeka1364 2 жыл бұрын

so is DNA fragment and DNA probe the same thing at 4:00

@MissEstruchBiology 2 жыл бұрын

Nor exactly, a DNA fragment is a small piece of DNA. a DNA probe is a short single stranded piece of DNA that is labelled radioactively or fluorescently

@saniyasiddeka1364 2 жыл бұрын

@@MissEstruchBiology ohh so the DNA fragment becomes a DNA probe once radioactive/fluorescent label is added

@fredsbugs3045 2 жыл бұрын

@@saniyasiddeka1364 I'm pretty sure the DNA fragment is more similar to an allele (just a fragment seems to be larger). so similarly to a primer in a PCR, the probe attaches to the siDNA (single stranded) and scans along it until the desired allele is found then attaches to it, therefore allowing scans to identify the allele. DNA fragments are referred to in PCRs not DNA probes, where primers attach to the start of a DNA fragment within a siDNA (so the primer is also single stranded) allowing an enzyme to then bind called taq polymerase to then create a new strand of DNA starting from the primer if that makes sense.

@fredsbugs3045 2 жыл бұрын

Though my question would be, knowing that a primer isn't the same size as a DNA fragment as it only attaches at the start, is a DNA probe the same size as an allele? like the same number of nucleotides along? or does it not need to be the same size as long the probe is complimentary to part of the allele? thank you

@jamesklieve4620 3 жыл бұрын

Hi Miss, I have a quick question as I can't get in touch with my teacher for a while, she has recently sent us this topic list for our personalised upcoming exam: 1) Cell Biology 2) Organisation (is this just how we organise things into cells, tissue, organs etc etc? Or taxonomy?) 3) Infection and Response 4) Bioenergetics 5) Homeostasis and Response 6) Inheritance, Variation and Evolution 7) Ecology 8) Practical Skills Am I right in only focussing on Topic 2, the second half of Topic 4, as well as Topics 5, 6 and 7? Completely ignoring Topics 1, 3 and 8? Looking at the topics and their content, I believe this is right, but being both an anxious and clumsy person I fear missing something out and so am not so sure. . .

@MissEstruchBiology 3 жыл бұрын

Hello, I would say so, but I'm not quite sure what is meant by organisation. You're correct that it refers to cell, tissue, organ etc.. but your teacher may mean know the details of different organs and tissues, which is in topic 3. Good luck with your exam 🤞🤞