MS Session 7 - A Basic Introduction to Tandem MS

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AutoVectis Suite - Mass Spectrometry

Күн бұрын

A basic introduction to some key concepts in tandem mass spectrometry: the Product Scan, Precursor Scan, Neutral Loss Scan, Selected Reaction Monitoring, Data Dependent Analysis (DDA) and Data Independent Analysis (DIA).
In this video, we go though all the basic areas, one at a time, explaining what they are and why they are useful.
there are so many different DIA methods that we can't go through them all - but in this video I do explain the basic concepts of PAcIFIC and MSe.
If there are other areas you would like to see covered, then make suggestions in the comments.
This is part of a postgraduate level course, but may be of interest to viewers at different levels. You can also access the html5 version here: www.kilgourlab.com/postgrad-le...

Пікірлер: 3

@kodchakornuengchuen4724 3 ай бұрын

Very help me for QE exam! Thank u ❤❤❤ good explanation

@ms-ds3wv 2 жыл бұрын

Very nice explanation of tandem MS. Finally an explanation that gives the topic proper covering, the half-assed explanations we received in a MS course I took gave minimal context. The explanations in this video on the other hand were at lot easier to follow. What tandem MS does is adding a second ionisation step, so this opens up for combining an initial soft ionisation like electrospray with minimal fragmentation, with a more energetic second ionisation that produce fragments that can be traced back to the parent molecules. So we get both the benefits of soft ionisation with well defined peaks from mostly intact molecules as well as an oppourtunity to extract more structural information that can be read from the fragments that comes with more energetic ionisation.

@autovectis 2 жыл бұрын

Thanks for the comment! 😁 And, your are right, some tandem MS methods are also identical or almost identical to types of ionisation - like EID is using the same effect as electron ionisation and UVPD is using the same effect as photo-ionisation. But, the most common type of tandem MS, collisionally induced dissociation, isn't directly related to a type of ionisation - it and infrared dissociation are really just methods of heating molecules up until some bonds break. There are even some combination methods where you first heat ions up a bit (using collisions or lasers) before you hit them with another fragmentation technique. Poor ions - but tell me everything you know!