PARAMECIA grown at home - TIPS and TRICKS for your microbe zoo! 🔬 224

Рет қаралды 24,838

2 жыл бұрын

How to make an enrichment culture for ciliates, such as Paramecium. Microscopy advice, tips and tricks.
[1:37] Step 1 - Collecting Paramecia
[2:06] Step 2 - Add food
[2:51] Step 3 - Wait for them to multiply
[3:13] Step 4 - Prepare the microscope slide
[3:45] Step 5 - Observe
[4:12] Tip 1 - Oxygen concentration
[5:12] Tip 2 - Crustaceans
[5:28] Tip 3 - Age of water
[6:24] Tip 4 - Progression of microorganisms
[6:54] Tip 5 - Types of food
[7:30] Tip 6 - Replenishing water
[8:00] Tip 7 - Safety
[8:16] Tip 8 - Limiting movement
[8:33] Tip 9 - Different locations in sample
[9:23] Tip 10 - If all fails.....
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Пікірлер: 70

@billinct860 2 жыл бұрын

Many years ago, I had several aquariums and raised Angelfish from their eggs. I first fed them Paramecium cultured in a large jar of water from one aquarium, along with a small handful of dried hay. In a week or so there were millions of Paramecia. Each culture thrived for about a week before dying off, but a small sample would start a new population easily. Quite stinky, so I covered the jar with paper towels and kept it in a dark place in my cellar.

@haiijinks3569 2 жыл бұрын

I just recently found your channel, and its amazing!! It had really helped spark my interest in the microbiology world. I am getting a microscope for christmas, and I am counting down the days till I can do this for myself. Thank you so much for sharing this wonder with the world!!!

@osterberg87 2 жыл бұрын

Thank you so much. I like all of your videos but this is the best. Maybe you can talk more about cultivation of things larger then bacteria.

@zamioculcaszamiifolia3646 2 жыл бұрын

Thank you so much Oliver! You helped me get the most out of my microscope. Your videos are really thorough and fun!

@namnueaintarabut7348 2 жыл бұрын

The paramecium are good for a baby fish too

@H_E_M01 3 ай бұрын

I’ve been searching all around KZfaq for a good video about paramecium. And this is the only one who has a step by step tutorial. You saved my life, 10/10 🏆

@JackCrow0893 2 жыл бұрын

I just got a microscope, not a big one by all means, just a nice medium sized one that I love, when I was little boy my father got me a microscope and I was in ore of all the life their was in just one drop of pond water..... I'm now 65 years old and now I can go back to my hobby.... micrology...love your page... I subbed you, Thank you for all you do. Chris.

@JB-ol4vz 2 жыл бұрын

Thank you for your tips. I have saved moss and water from my forest to have during the winter in a aquarium. The number of organisms have decreased the last coupled of weeks but now you have give me a lot of tips to increase them. I will also go out and take some ice from the frozen Forrest swamps and see if life can ree start when been in ice. Cheers from a frozen Sweden.

@davidonfim2381 2 жыл бұрын

You don't even need to take water samples, you can just go to a dried up pool or to the edges of a pond and grab some dirt and dried up grass, then put them in some water. The dried up paramecium cysts/eggs/whatever they are will eventually hatch and colonize the container. I guess that takes longer, but I've never tried to compare methods.

@ummehanihassi4513 2 жыл бұрын

Very fascinating! Thank you for the tips 😁

@Jjjjjjjjjjjjjjjjjjjjjjjjjjjjjs 5 ай бұрын

You do such an excellent job of explaining things! I can’t wait to open my microscope on Christmas and start looking at the samples I’ve been collecting! 🔬

@ivancho5854 4 ай бұрын

7:45 If there is chlorine in the tap water then you are correct, it will evaporate, however most tap water today contains chloramine which will not evaporate and will kill most organisms in the water. Bottled water should be safer. Excellent video. Thank you.

@RobertoCighetti 2 жыл бұрын

Thanks for the tips, really interesting and useful! Amazing video

@larryhazelwood2632 Жыл бұрын

I set up a hay infusion once and was fortunate enough to find a few paramecium cadatium in a pond sample. The hardest part of the process was collecting them from the jar which had a bright light behind it. Over the years I could tell by the shape and locomotion of the organism through the water so I would collect them with a fine pipette. I was very successful culturing them and had no other protozoa. Thanks for sharing brings back great memories.

@jasonmoon5040 2 жыл бұрын

Always a pleasure hearing from you Oliver! Learn a lot from each of your videos and super grateful! Anatomy of different types of microbes is something I am trying to study in spare time. Maybe a video sometime in the future that touches on some of the basic anatomy. I’ll be in the front row! Until then happy microbe hunting!

@zanderdaugherty7389 Жыл бұрын

I got great results in 3 days. This channel is great! : )

@Microbehunter Жыл бұрын

:-)

@metiusabt2581 2 жыл бұрын

Kore dankon, Oliver. Tre utila!

@JohnSmith-kf1fc Жыл бұрын

Thank you for the tips! This answers the questions i had. My next hunt will be successful i cant wait to go to the pond!

@seaham3d695 2 жыл бұрын

Love you man x!

@erikacowen9794 Ай бұрын

I was going to buy a paramecium culture on ebay to feed some Rainbowfish fry I am hoping to breed, they are asking £15 for this. Now instead, I am going to our local Wetlands pond with my dog. Thank you Oliver :)

@AussyCC 2 жыл бұрын

Nice vid and info again thx mate. Keep up the good work @microbehunter

@Westartedgeneverse 4 ай бұрын

Your respected thanks for the info

@trinacorbett4827 2 жыл бұрын

!!! ... I am so happy to find you ... !!!

@miguelangel3029 Жыл бұрын

I congratulate you for your videos. I would like to know what microscope you use and what camera you use to make those magnificent videos. Thank you.

@TejhunnRamchurn 2 жыл бұрын

Hello Oliver, thank you for your sharing of knowledge. Can we have a video on inverted microscopes?

@chimeradiego4276 Жыл бұрын

NEW subscriptor, thanks a lot!!

@shankrprajapat1162 2 жыл бұрын

Best 🤘🤘

@IdiotWithEducation 2 жыл бұрын

How did you get these shots!? I would love to know everything you used for the ultra clear view and also the different color contrasts you used! Is it phase contrast or DLSR?

@Curixq 2 жыл бұрын

Is there an equivalent of paramecia for salt water?

@pmate95 2 жыл бұрын

Hi Oliver! What kind of microscope do you use for these videos? Did you use some kind of special illumination, like DIC? These are very good videos, congratulations on it!

@Microbehunter 2 жыл бұрын

Yes, it is DIC. And also Phase Contrast.

@pmate95 2 жыл бұрын

@Microbehunter I would like to buy a DIC type microscope, but I can't find it for commercial use anywhere. Where did you buy yours, and what is the exact type of yours?

@Microbehunter 2 жыл бұрын

DIC is an advanced technique. If you want to get one, then you have to directly contact Olympus, Nikon, Zeiss or Leica for an offer. I once asked Euromex for an offer (they are by far the cheapest) and a DIC microscope from Euromex (the Delphi-X) costs about EUR 12000. For the average amateur, this is out of range. I therefore recommend that you first try out Oblique Illumination, which also gives similar results, but which costs nothing (only a small cardboard filter).

@WillettaWhite Жыл бұрын

@@Microbehunter Van you please examine a sample for me ?

@lisabryna839 2 жыл бұрын

wowww

@dotzoomchannel 2 жыл бұрын

What kind of your microscope and what type? May i know that?

@yonetmen 2 жыл бұрын

I am still waiting for Oxytricha! :)

@____.__._.._ 2 жыл бұрын

Hello @Microbehunter , sorry for unrelated question, but does Vorticella exists in "free swimming" form, instead of a typical form attached to something? When i type Vorticella in qoogle scholar i see many papers on the contracting stalk, but none about the attaching mechanism. By the way, great video as always

@Microbehunter 2 жыл бұрын

Vorticella can do both. The free swimming form will grow a stalk and attach.

@zamioculcaszamiifolia3646 2 жыл бұрын

I just saw one in that form what a coincidence!

@____.__._.._ 2 жыл бұрын

@@Microbehunter Thank you! i have many photos of attached ones, but only one vid about a mature vorticella detaching and swimming

@____.__._.._ 2 жыл бұрын

@@Microbehunter Hello, just another question that popped into my mind. When one paramecium cell divides, there are quite complicated divisons of both nuclei, and how about the rest of the cell, like contractile vacuoles? They doesnt looks like something simple that could be just "cut in half"?

@lanphanh 2 жыл бұрын

Sir, What creatures are at the 6:42 second? I found a lot in my infusoria culture. Is it good or bad for my fish fries? Will they eat them?

@Microbehunter 2 жыл бұрын

These are Rotifers. Very common.

@askar_arystanbayev Жыл бұрын

How many X microscope do you use?

@matthewwagner47 2 жыл бұрын

@4:14 What was that big worm looking thing on the left side?

@Microbehunter 2 жыл бұрын

Flatworm

@matthewwagner47 2 жыл бұрын

@@Microbehunter thanks for response. ugh.... I see exactly why you have to boil water before you drink it. Its truely amazing whats in a drop of water.

@hanswasgaming3547 Жыл бұрын

Would Cheese be good? Maybe isn't the best to look at cheese mold ;-;

@TheRealDXVINCI_PLAYA Жыл бұрын

Hey microbe hunter, is there any food that might kill paramecium? Bc I gave mine a club cracker and when I checked a few days later, there was none.

@Microbehunter Жыл бұрын

Too much salt on the cracker?

@TheRealDXVINCI_PLAYA Жыл бұрын

I’m not sure

@TheRealDXVINCI_PLAYA Жыл бұрын

Wait yes there is

@TheRealDXVINCI_PLAYA 11 ай бұрын

@@Microbehunterould a rice crisp cereal work?

@ignaciogomezlima3416 Жыл бұрын

its brown sugar a suitable food for bacteria?

@Microbehunter Жыл бұрын

Depends on the bacteria, but generally yes. If too much sugar present, then bacteria will lose too much water due to osmosis and this is also not good for them (high sugar concentration preserves food, eg. jam etc.)

@ignaciogomezlima3416 Жыл бұрын

@@Microbehunter That is really interesting, so only low quantities like a special snack.

@asiyabegumshaikh9298 Жыл бұрын

At which magnification we can observe it under compound microscope????

@Microbehunter Жыл бұрын

They can be seen starting 40x or better at 100x or 200x.

@asiyabegumshaikh9298 Жыл бұрын

Should we covered the jar with lid or leave it open????

@Microbehunter Жыл бұрын

@@asiyabegumshaikh9298 Leave open. very important. Otherwise it might start to rot and smell bad, and many bacteria start to grow. When water evaporates, add more water.

@asiyabegumshaikh9298 Жыл бұрын

Thanks ✨

@asiyabegumshaikh9298 Жыл бұрын

Thank you so I've got many paramoecium in my culture 🧫 I've 1 question how long we can keep them alive?????