Performing Column Chromatography

Рет қаралды 82,973

Жыл бұрын

We just learned about thin layer chromatography, but a much more industrially relevant technique is column chromatography. This is how we will separate large quantities of compounds in a mixture by virtue of their differing polarities. It follows the same principles as TLC, but we are pushing compounds down a column of silica gel rather than up a TLC plate. Let's check it out!
Visit Thermo Fischer Scientific for all your laboratory needs: www.thermofisher.com/lab-chem...
Follow Jamal Muhoza on social media: linktr.ee/dr_Jamal
Watch the whole Chemistry Laboratory Techniques playlist: bit.ly/ProfDaveChemLab
General Chemistry Tutorials: bit.ly/ProfDaveGenChem
Organic Chemistry Tutorials: bit.ly/ProfDaveOrgChem
Biochemistry Tutorials: bit.ly/ProfDaveBiochem
Biology Tutorials: bit.ly/ProfDaveBio
Classical Physics Tutorials: bit.ly/ProfDavePhysics1
Modern Physics Tutorials: bit.ly/ProfDavePhysics2
Mathematics Tutorials: bit.ly/ProfDaveMath
EMAIL► ProfessorDaveExplains@gmail.com
PATREON► / professordaveexplains
Check out "Is This Wi-Fi Organic?", my book on disarming pseudoscience!
Amazon: amzn.to/2HtNpVH
Bookshop: bit.ly/39cKADM
Barnes and Noble: bit.ly/3pUjmrn
Book Depository: bit.ly/3aOVDlT

Пікірлер: 61

@sjzara Жыл бұрын

I was shown around the department at the start of my biology Ph.D. first thing I did in that laboratory was accidentally knock over someone’s column chromatography experiment.

@entropy-1 Жыл бұрын

It is ok friend we are all human

@VaughanMcCue Жыл бұрын

Prof Dave warned you to use TLC. Lab gear has feelings and needs tender loving care.

@JamesVestal-dz5qm Жыл бұрын

Oooooh bad!

@watchapgang7150 2 ай бұрын

пиздец

@quinterotieno4774 24 күн бұрын

Oooo Noooo

@martinarenzi4744 Жыл бұрын

Thank you! I am so happy that you made this playlist. I am looking forward all the spectroscopies and mass spec. I love to see all the lab procedures through theese videos and their applications in real life analyses. Thank you Professor Dave ❤

@caseyr803 6 ай бұрын

Oh my god. I am currently a PhD student in ochem and I didn’t actually learn what a column is (my undergrad was not quite on the level of most institutions). My current research lab has an auto column but I’m also just very early in my research so I’ve only used it once and don’t quite understand the theory if it. This is my first time seeing the theory and it truly reminds me so much of why I love chemistry. So interesting!

@borttorbbq2556 Жыл бұрын

It’s because of you and a few other chemistry channels I follow but I do actually get home ads for lab equipment pretty often

@waelfadlallah8939 Жыл бұрын

Thank you for showcasing the basics of this technique and ending by displaying the latest advancement in this field :)

@stephenwalton8507 Жыл бұрын

Interesting video. I used to run flame ionization chromatographs to analyze hydrocarbon gas samples.

@Thaumius Жыл бұрын

One point to add, please dont manipulate silica without a respirator, its a inhalation hazard, especially doing the wet technique.

@crickerloverwithlove 5 ай бұрын

Class 11th experiment teacher said do it your self in lab now I understand this❤. Thanku

@RafaelSilva-by7yz Жыл бұрын

Hello teacher, how are you? Could you explain in detail, if possible, how to select the best column based on silica density? Thanks for the video

@Goose-ib2ti Жыл бұрын

Good stuff. You should do a video on GC!

@martinlam5161 Жыл бұрын

May I know the type of silica gel used in this video, please? Thanks!

@anub492 2 ай бұрын

good one❤

@defeatSpace Жыл бұрын

Fun stuff!

@wangchuanqi3942 Жыл бұрын

I usually prefer to add sand before I load the sample. It is less likely to disturb the silica gel level with sand on top.

@ashigaruvolt6548 Жыл бұрын

Thermofisher sponsorship? Let’s go

@Mikich22 Ай бұрын

Thanks very much for a good example of column chromatography. Can you tell a concentration of methyl red and bromocresol green in your sample? Or suggest a concentration. I need this for laboratory practice for students.

@Kalashnikova_Val 2 күн бұрын

Could you please explain why the green compound eluated first if its more polar and had to stay longer on the polar silica gel?

@eliottjung4568 Жыл бұрын

Solid loading team

@paulmoran7026 Жыл бұрын

I’ve done 100s , possibly 1000s of columns, but nothing beats doing cryogenic columns over 24-48 hours with sensitive precious metal compounds.

@matte4123 Жыл бұрын

lysergic acid is much more fun

@pahadipreet5812 11 ай бұрын

Thanku 😊

@rashaselem 2 ай бұрын

شكرا لك

@lucidity1 Жыл бұрын

that's cute, when do you discuss HPLC? it's my favorite.

@manthandambhare7395 11 ай бұрын

Awesome

@JamesVestal-dz5qm Жыл бұрын

Lamont taught me that the first channel you turn on in the morning ought to be your occupation!

@GhulamAbbas-ci5zz Жыл бұрын

Start a paleontology series

@user-lo1hj3zo3n 9 ай бұрын

Thank you. your videos are very useful and interesting. just please speak in lower speed. so that people like me that English is their second language will understand better. Thank you Professor.

@rassimsimou1594 Жыл бұрын

Good

@erikamogelvang6171 Ай бұрын

URGENT question, 25 mL of solvent was added to what mass of the silica gel powder?

@Artavazd.kirakosyan 2 ай бұрын

thank you very much. quick question: the solvent that is used to dissolve the compounds (6:59), is it same as the eluent?

@Noldor97 2 ай бұрын

So you could do that. keep in mind that you really want to use a solvent where your sample is fully dissolved in as little of solvent as possible.Most of the time it doesnt really matter if you use some other solvent here, as its getting diluted so fast in the column that it doesnt really matter.

@Artavazd.kirakosyan 2 ай бұрын

@@Noldor97 thanks a lot.

@stephan5279 Жыл бұрын

Ohh... Column Chomatogaphy... The fear of every person which does synthesis...

@Noldor97 2 ай бұрын

what do you mean? ding columns is great:D

@nirmalmishra6404 Жыл бұрын

Sometimes you are Badass, sometimes Dick, you are non other but professor David.

@colinprice712 Жыл бұрын

Please, please, don’t just turn the tap on any burette with one hand! You can put a lot of strain on the thin tubing joint between the tube and the stopcock. If it’s just a little stiff you can break the stopcock off spilling solvent everywhere let alone the risk of glass fragments… And yes, that’s from experience…

@WKUConsulting Ай бұрын

may I use pieces of this video for a youtube training seminar? I will of course credit you and fischer. as well as link your channels.

@ProfessorDaveExplains Ай бұрын

Yeah if you're just playing the video on a KZfaq player of course

@WKUConsulting Ай бұрын

@@ProfessorDaveExplains thank you!

@JamesVestal-dz5qm Жыл бұрын

Boy you can't get no higher than this!

@maheraberu2000 Жыл бұрын

The problem is that producers of beads like thermifischer unfortunately don't say what is inside the slurry of the beads and that makes sometimes problems. It's extremely expensive too

@ProfessorDaveExplains Жыл бұрын

Of course they say what is in it. Why would you think that people don't know the composition of silica gel?

@Blevic18 2 ай бұрын

What kind of sand?

@Noldor97 2 ай бұрын

normal clean seasand. you can buy that at every chemical supplier

@ObichereGolden 3 ай бұрын

Please what is the need for the sand?

@Noldor97 2 ай бұрын

Okay so: sand at the bottom to prevent silica from getting in your samples that you collect. Sand at the top to prevent to disrupt your silica when you are readding your eluent