This video describes the "Phased Cleanup" step for NEBNext UltraExpress™ Kits, this step is a new cleanup method that improves separation of the library from smaller fragments, including adaptor dimer.
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@PooleySteen7 ай бұрын
If someone at your laboratory deliberately left 5 mL of T7 exonulease gene 6 out at room temperature for one hour what would said to them?
@nebiolabs7 ай бұрын
We’re sorry to hear about your enzyme! At NEB, we always like to assume benign intent, so perhaps your lab mate was checking whether your stock of the enzyme was heat labile? We recommend reaching out to the enzyme manufacturer’s Technical Support team to see if they can help!
@PooleySteen7 ай бұрын
@@nebiolabs No just a fifty five year old acting like a child, because he didn't like a new protocol. As for contacting technical support they would be some biochemical wizards if they could put it back together, but more then happy to receive 11,000 dollars for new stock.