Congrats! always a reference in molecular biology and genetics! We get your NEB products here in Mexico and use your great online tools! Keep up the good work!!

how do I actually know the microliter required for the 5-10 unit, say if I have a 10,000 unit/mL RSAI

Hi mam! I have a problem I extracted the plasmid and did digestion for many time Always got no band after digestion or sometimes not properly digestion What is the possible reason behind this I did this many time all times failed

Hi, I extracted the plasmid and ran the gel and got good Bands of plasmid but I did not get band after restriction enzyme , did not properly digested my plasmid What is the possible reason behind this? Please guide me I did it many times

how would I go about simulating a restriction digest when I have my plasmid sequence in one file and my DNA insert sequence in another file? Would I just paste the sequences one after the other?

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If someone at your laboratory deliberately left 5 mL of T7 exonulease gene 6 out at room temperature for one hour what would said to them?

Can you share the Notes to get knowledge of Gibson Cloning

Every time I try to do this, a loading icon shows up and disables me from doing everything. I've heard others say the same, this doesn't work.

Thank u sir it is very helpful!!!!!!!

great video, very well explained and easy to understand :>

thank you sir

Very interesting video, thank you.

I love it

Cell free protein means protein synthesis in a test tube????

Ai cho xin tên bài nhạc này dc k nhạc hay

i come here every pi day. hi chris!

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How far does the exonuclease chew the DNA? How does it know when to stop?

I need help with my experiment please help, I am trying to add enzyme to the PLA and observe the degradation

Thanks a million

Well this aged well..get tested as much as possible!

How many beads are used for blood collection in glass bead tube?

Is it 100ng per microliter or is it in the total volume?

What is the implication of preserving kidney tissue at -20 degrees Celcius, if there is any?

The Gibson assembly does not faithfully join linear fragments. It needs a backbone vector to in the master mix. If only insert fragments are used it will also chop the outward 5' to 3' sequences from both strands, resulting in a single but truncated PCR product whose ends are shorter.

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Hi, thank you for your focus on Mansonella infections. Do you offer testing in the US? It's been several years since this video was posted- have you added testing for Mansonella Streptocerca or the new clad of M. Perstans DEUX, and if not, do you plan to ?? Do you currently offer testing to consumers or only to medical professionals? Thanks!

Sip wes

Crazy stuff.

Is it necessary use the loop primers?

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My Life revolves around CRISPR Thanks for the video

that smile killed me..

video anda sangat bagus dan mempunyai mesej yang luas terima kasih

Hello, does this also apply to the role of DNA ligase in DNA replication?

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This was so informative...great video!!

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Whole genome PCR and mini-primer PCR Also make a video on these 2 topics . Thank you.

The video sound is pretty good, beyond my imagination

Some segments in the video are stamped not adjacent to each other

Gee.....the animals in the u.s. can get Mansonella perstans......and so when that midge is a carrier, and bites a human, we can't get it? Are you freaking kidding me???? Guess how many people ended up in the E.R. in Olympia Wa with Midge bites that wouldn't heal, and the bite area had to be gored out with a scalpel, and gauze soaked in antibiotics was then inserted into the wound, and had to be pulled out an inch every day, and on the third day, it all had to be pulled out. Was tested for MRSA and negative. I almost had to be given an IV of antibiotics. Guess how many people? I was bitten in the groin, and ten years later, just had a 7.33 lb abdominal tumor taken out, no cancer. I had caught one that was biting, a year later to the month, and bought a microscope. It was a biting Midge, Culicoides.

What about post translational mods?

The devils seed…MRNA and nanotechnology

Got it

Does glass beads increasing the efficiency of transformation?

How to extract DNA from clotted blood(Yellow blood clotted tubes)