Preparation of nutrient agar plates l How to make agar plate

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2 жыл бұрын

In microbiology, it is very important to know how to make nutrient agar plates for routine microbiological work. Nutrient agar plates are prepared to grow microorganisms, especially bacteria.
Visit blog for more details: rbrlifescience.com
Hi, I Am Rahul B Rathod, Welcome to Our You Tube Channel RBR Life Science
About this video-
friends, In this video tutorial we are going to learn How to make nutrient agar medium plates and preparation of nutrient agar in petri plates for microbiology laboratory work thank you so much ....
Queries Solved:
1. How to make agar medium plates
2. How to sterilize media using autoclave
3. How to pour media into Petri plates
4. How to store agar plates in refrigerator
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#Nutrient Agar
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#rbrlifescience

Пікірлер: 108

@modoumangan9851 Жыл бұрын

Awesome, very clear and easy to understand

@rbrlifescience Жыл бұрын

Most welcome, also please suggest any topic or concepts i should make such videos

@modoumangan9851 Жыл бұрын

@@rbrlifescience I will appreciate it if you make a video about putting bacteria broth into microtiter plate to measure their growth curve and optical density

@sindhujan5241 2 ай бұрын

The best explanation out there. you are awesome 💯❤ ❤

@rbrlifescience 2 ай бұрын

Thank you Dear

@Vjsnd Жыл бұрын

This is great for beginners in the lab like myself :) Loved it!!! Thank you

@rbrlifescience Жыл бұрын

Glad to hear that ... stay tuned to watch such more videos

@upeducationtec9064 4 ай бұрын

thank you very much. well understood

@dakavlogs9680 4 күн бұрын

Casi no entendí pero las imágenes ayudaron mucho para comprender y me siento mas seguro para hacer mi examen de esto

@rbrlifescience 4 күн бұрын

English please

@greenworld1562 Жыл бұрын

Well explained

@himalayanwizard 4 ай бұрын

Youve got infinite flask bro

@santoshbhavi8516 2 жыл бұрын

Thank you very much for this informative video.

@rbrlifescience 2 жыл бұрын

Most welcome sir

@viratkohli7137 Жыл бұрын

Thanks sir

@lovelyfeespanola7931 4 ай бұрын

Why do some nutrient gar need to add sodium for prep?

@manand287 2 жыл бұрын

Thanku so much for u r hlep of my studies..god bless u

@rbrlifescience 2 жыл бұрын

Most welcome Sir

@soniyamourya8351 2 жыл бұрын

Sir really this video use for me👍

@rbrlifescience 2 жыл бұрын

Welcome Soniya ji ...You can also suggest topics and I will try to make videos on it 🙏keep it up

@sawairagul251 2 жыл бұрын

Well explained 🥰

@rbrlifescience 2 жыл бұрын

Most welcome..And stay tuned

@sudiptakapri8624 10 ай бұрын

1.After making the nutrient broth we should check the the pH . 2. Before pouring the plates conical flask neck should be heated for contamination safety

@rbrlifescience 10 ай бұрын

Yes, correct

@rulakakatipandey9554 Жыл бұрын

Nice and stepwise and easily understanding to all....thank u sir

@rbrlifescience Жыл бұрын

Thank you for complement..... Stay tuned for such more videos

@greenworld1562 Жыл бұрын

Your videos are very helpful. Thank you sir, plz make more video of lab experiment

@rbrlifescience Жыл бұрын

Sure, please suggest me topics or concepts on which I should make videos

@ManiMani-qj8rb Жыл бұрын

Petri art video panuga

@lvs8850 8 ай бұрын

very good video

@rbrlifescience 8 ай бұрын

Thank you

@vlogwithsanu377 Жыл бұрын

Thanku 🥰🥰

@rbrlifescience Жыл бұрын

Most welcome

@ruchika_899 Жыл бұрын

Tysm ❤

@rbrlifescience Жыл бұрын

Most welcome and stay tuned for such more videos

@user-ju3iw8lk6f 7 ай бұрын

Thank you ❤

@rbrlifescience 7 ай бұрын

Welcome

@divya821 2 жыл бұрын

Sir make more how to reporting culture sensitivity and biochemical reaction

@rbrlifescience 2 жыл бұрын

Ok i will try

@poleenterprise7676 5 ай бұрын

Thanks 🙏

@rbrlifescience 5 ай бұрын

You’re welcome 😊

@Wrcher 5 ай бұрын

Thank you

@rbrlifescience 5 ай бұрын

You're welcome

@anassabduabdu9798 Жыл бұрын

Well done doctor

@rbrlifescience Жыл бұрын

Thank you and stay tuned to watch such more videos

@rbrlifescience 8 ай бұрын

I am just Master of Zoology.

@joyamedu5680 Ай бұрын

After preparing the Agar solution, can i use it to culture bacteria within 48 hrs without putting it in d refrigerator?

@rbrlifescience Ай бұрын

Yes you can use plates after 24 hrs of incubation

@arielbowmanoquinn7065 Жыл бұрын

I cracked up when he kept showing the petri dish pouring. 🤣 Classic.

@rbrlifescience Жыл бұрын

Thank you that you liked it

@kolavinodha1242 10 ай бұрын

Sir can u make the vedio regarding the Isolation of fungi nd bacteria from spoiled fruits nd vegetables sir please

@rbrlifescience 10 ай бұрын

The process is more or less similar as explained in the video. You can use your sample instead of soil

@a110100 9 ай бұрын

Transfer a piece of the fresh fruit onto agar. Hopefully they grow separately and you can keep transferring away from the contamination.

@somisettyharshitha7821 2 жыл бұрын

Sir make isolation and identification of probiotic bacteria and yeast

@rbrlifescience 2 жыл бұрын

i will try

@divya821 2 жыл бұрын

Sir make for culture sensitivity blood urine and so on

@rbrlifescience 2 жыл бұрын

ok i will try

@UyenTran-ut5sy 2 ай бұрын

sir, why unused sterile medium should be then stored in the fridge rather than at room temperature condition?

@rbrlifescience Ай бұрын

The moisture from the agar medium will evaporate, and plates will become dry. For microbial growth to occur we need water in the agar plate. Also if we keep the petri plates outside then there will be chances of microbial contamination.

@kael3808 4 ай бұрын

hi! what can we do if we’ll just sterilize the agar using pressure cooker? (we don’t have autoclave)

@rbrlifescience 4 ай бұрын

Yes it will sterilize the agar

@HattoriHanzo62 Жыл бұрын

just a small and respectful comment: the correct measure unit to indicate "grams" is "g" and not "gm"

@rbrlifescience Жыл бұрын

Thank you for suggestion. I accept and I will definitely try not to write this gm for gram unit . Apology

@HattoriHanzo62 Жыл бұрын

@@rbrlifescience no need to apologize, 🙂everybody can make small imperfection: the quality of the information is great and I just wanted to help to be more precise

@rbrlifescience Жыл бұрын

@@HattoriHanzo62 👍👍

@ligayan5143 Жыл бұрын

sir how do u compute for the powder agar to be used?

@rbrlifescience Жыл бұрын

If you are using ready made nutrient agar bottle then there is no need for computation. If you are preparing by addition of individual components then agar percentage is 1.5% For making 100 ml of NA take 1.5 g of agar powder For making 1000 ml of NA take 15 g of agar powder For exact composition read our blog post rbrlifescience.com/nutrient-agar-media-composition-and-uses/

@nishitachitkara4623 Ай бұрын

But why we use autoclaving or incubator

@rbrlifescience Ай бұрын

We use autoclave to setrilize and dissolve the media. After sterilization we pour the media in petri plate and after agar in plate solidifies we need to keep plates in incubator for drying for 24 hrs -48 hrs. This incubation is also check any contamination while pouring.

@RealKing213 Жыл бұрын

From where we can buy nutrition agar and can grow orchid seed

@rbrlifescience Жыл бұрын

You can buy the nutrient agar from various companies like Hi media, thermo scientific, Merck etc. Few are also available on Amazon

@fansarulsk4415 Жыл бұрын

Blood agar macconkey agar video upload karo

@rbrlifescience Жыл бұрын

I got your request.. and I definitely going to make the video on blood agar and macconkey agar soon.👍

@winwinkhaing468 Жыл бұрын

Why you convert plates? Is it really need or not ? Please explain kindly me?

@rbrlifescience Жыл бұрын

After pouring agar media in the plates once agar media in the plate solidify (usually after 1-2 hrs) then it should be kept in inverted position. Now two things are there, lid and plate with Media. If we invert the plate then due to weight of media in plate kept on lid there no air will enter inside the plate. If we keep the plate as it is after pouring then there are chances that air will enter inside and plate will be contaminated. This is the reason that all solid agar plates are always kept in inverted position in order to avoid entry of air inside the plate. Also plates are not contaminated while handling in inverted position.

@rachealpalmer9941 3 ай бұрын

Can I keep it for 48 hrs before refrigeration

@rbrlifescience 3 ай бұрын

Yes off course

@soniyarajkumari9419 Ай бұрын

How do we know that we need 4.6gm of NA in 200ml of H2O

@rbrlifescience Ай бұрын

On NA bottle, it is mentioned that for making 1000 ml of NA you need to take 28 grams of NA powder from bottle and suspend it in 1000 ml distilled water. It's just calculation 28 g for 1000ml 2.8g for 100 ml 4.6g for 200 ml Have a great day 😊

@zesseljhontaguibolos6621 Жыл бұрын

whathappen do them after 24 hrs, 42 hrs and 72 hrs?

@rbrlifescience Жыл бұрын

As the time passes the water in the agar gets evaporated if we keep plate in the incubator. A time comes when the agar falls from the lid after 5 to 6 days

@bernadettegaby 2 жыл бұрын

For 23 gm of nutrient agar powder, add 1 L of distilled water. Is it right?

@rbrlifescience 2 жыл бұрын

yes correct, whatever media you prepare there is information available on bottle itself. Take 23 gm of nutrient agar powder add it in 1000 ml distilled water

@hak8278 Жыл бұрын

28 mg for 1L👍

@rbrlifescience Жыл бұрын

It will depend upon which company media you are using. In this video i have added 28 grams of nutrient agar in 1 litre of distilled water. Instructions are always written on media bottle itself

@niloofarjadidi6030 Жыл бұрын

hi, what is the percentage of the ingredients of nutrient agar media?

@rbrlifescience Жыл бұрын

Nutrient agar media has different components such as Peptone, Beef Extract, Sodium Chloride, and Agar. For exact composition percentage please read our blog post rbrlifescience.com/nutrient-agar-media-composition-and-uses/

@gardeningonly 6 ай бұрын

Which is the good online platform to buy nutrient agar for india?

@rbrlifescience 6 ай бұрын

It's himedia labs

@gardeningonly 6 ай бұрын

@@rbrlifescience thank you i already checked there but they don't have 100gm I am just learning from your videos and blog so just want to try with small amount.

@rbrlifescience 6 ай бұрын

You can purchase from Amazon you will get 100 gm

@prabhadabahde1905 2 жыл бұрын

Sir humko 17 g Lena hai kaise weigh kare

@rbrlifescience 2 жыл бұрын

First take a weighing boat or dish and keep it on digital weighing balance and tare the weight of dish to 0 . Now open the nutrient agar powder bottle and add the Nutrient agar powder in dish till the weight on weighing balance shows 17 gram.

@prabhadabahde1905 2 жыл бұрын

@@rbrlifescience thank you sir

@rbrlifescience 2 жыл бұрын

@@prabhadabahde1905 most welcome

@chkumaryadav657 2 жыл бұрын

Nutrient Agar 37grams per 1liter so potatto Dextro Broth 24 Grams per 1 liter

@rbrlifescience 2 жыл бұрын

Whatever media you are preparing there is information available on Media bottle itself. Generally there is information given for how much media you should add to make 1 litre solution of that media .

@chkumaryadav657 2 жыл бұрын

@@rbrlifescience ok sir good information

@Talhanaqvi12 5 ай бұрын

But he didnt use sand for bacteria ??

@rbrlifescience 5 ай бұрын

its animation for understanding

@Pharmacygirl-struggles 4 ай бұрын

Because he's using nutrient agar powder.

@TechsScience 4 ай бұрын

I tried this Experiment but got no reasult Also my solution looks whitish

@rbrlifescience 4 ай бұрын

After autoclaving solution will be clear

@TechsScience 4 ай бұрын

@@rbrlifescience but the agar solution is yellowish In general My solution remains pale yellow even after autoclave & yes I am taking an edible agar (used to make jelly) is it OK?

@rbrlifescience 4 ай бұрын

Once agar in plate solidifies then it remains slightly yellowish. Nothing to worry about.

@TechsScience 4 ай бұрын

@@rbrlifescience it's not about the color the problem is I am not getting bacteria culture even after 7days