Publishing Flow Cytometry Data (Intro to Flow

Publishing Flow Cytometry Data (Intro to Flow - Episode 9)

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Күн бұрын

And at long last- the final episode in our Introduction to Flow Cytometry series! Flow cytometry is a powerful technique for studying single cells. Knowing how to properly present your data in a publication is critical to using the full potential of your flow cytometry data. In this video, we go over the components you should include in all publications and some tips to make your data looks its best!
Intro to Flow Cytometry Playlist: • Introduction to Flow C...
Link to slide deck: drive.google.com/file/d/1UQ-P...
Link to MIFlowCyt checklist: drive.google.com/file/d/1g9RC...
More info about MIFlowCyt: isac-net.org/page/MIFlowCyt

Пікірлер: 10

@user-zb4dk6yq1i 3 ай бұрын

Thank you Aja. The videos are great.

@user-tb6tj9tt1y 2 жыл бұрын

Aja- just wanted to say your videos are great. Thank you.

@MegaMuffinManX Жыл бұрын

Hi! These videos have been massively helpful. I'm not sure if this is too general or too naive, but could it be possible to do a video about how to read and interpret different flow cytometry plots?

@ajarieger_flow Жыл бұрын

I can do that! Stay tuned!!

@fatima.Hubaishi 3 жыл бұрын

Thank you so much Aja! I have question, when we take the frequency of certain population ex. CD4 for negative and positive groups. What is the best way to represent the cell number of the CD4 so I can compare statistically? I saw people do it from total cell counts that they got by trypan blue but other normalize a fixed number like1000? I hope I explained my question well.

@ajarieger_flow 3 жыл бұрын

Thanks for your question! When representing cell counts as absolute counts, I prefer not to apply the % gated to trypan blue counts. There can be a lot of inaccuracy there. Instead, I recommend either (1) adding a counting bead to your sample and use this to determine the absolute counts or (2) determine the amount of volume run in a sample (some cytometers will give you this value; others you will have to determine this empirically) and use this volume and the cell numbers collected to calculate your absolute cell counts. Hopefully that answers your question!

@sachingcopk 3 жыл бұрын

Please make a video on gating strategy with flowJo, its very confusing for beginner

@ajarieger_flow 3 жыл бұрын

It is on my list!

@laurazarragavargas910 2 жыл бұрын

Please, can you explain how we can know if a very few events in a gate are real events? I love your work. Thanks for the videos.

@ajarieger_flow 2 жыл бұрын

Hi Laura! I would recommend looking at this paper: Allan AL, Keeney M (2010) J Oncol This image summarizes: drive.google.com/drive/u/1/my-drive In terms of determining if few events in a gate are real, I like to use supplementary techniques for this. One of my favourites is the ImageStream- as they say, seeing is believing :)