Interpreting flow cytometry data plots

  Рет қаралды 2,518

Aja Rieger

Aja Rieger

Күн бұрын

If you are new to flow or wondering what all these data plots mean, this video is for you! Herein we cover how to read basic flow cytometry plots (histogram and dot plots), how to understand a gating strategy, and how to interpret dimensionality reduction plots like tSNE and UMAP.

Пікірлер: 8
@Riasat202
@Riasat202 Ай бұрын
Excellent explanation!
@nghinguyen8045
@nghinguyen8045 Жыл бұрын
Thank you so much for the clear explanation.
@MegaMuffinManX
@MegaMuffinManX Жыл бұрын
Excellent! Thank you so much!
@Ching-WenLin
@Ching-WenLin Ай бұрын
Thank you Aja for the wonderful information - May I ask for a tutorial of how to export the MFI (fluorescence intensity) and perform statistical analysis and make figures out from it? Thank you!
@ajarieger_flow
@ajarieger_flow Ай бұрын
Thanks!! I have a video on statistics- check that one out. If you still have questions, let me know
@djhell3054
@djhell3054 Жыл бұрын
Thank you :)
@jelagatemily3119
@jelagatemily3119 Жыл бұрын
Hello Dr Aja, what did you use for your umaps? R or Fcs recently started working with umaps and made my first umaps with fcs and they are pretty I would love to see more videos of umaps. I couldn't manage to use flowAI but I did my cleaning and compensations on flowjo then exported the files to fsc. More videos on this area of unsupervised analysis will be highly appreciated
@ajarieger_flow
@ajarieger_flow Жыл бұрын
I used the FlowJo plugin for UMAPs, which runs through R but lets you stay in the FlowJo interface. I will work on making more videos on unsupervised analysis
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