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Recombinant Protein expression in E.coli, Best suitable strains for protein expression, advantages of using E.coli for choosing the host for protein expression.
0:00 Introduction
0:07 Protein Expression in E.coli
0:12 Advantages of E.coli System
1:45 E.Coli Strain & Application
2:23 Vectors for Protein Expression & Features
2:53 pUC19 Vector
3:08 Most widely used vectors
3:13 Cloning & Screening
3:26 Plasmid Extraction / Isolation
4:21 Restriction Digestion & Ligation
5:08 Restriction Digestion and Gel
5:15 Transformation and Expression
5:21 Transformation - Procedure
6:20 Shake Flask Culture- Protein Expression & Analysis
6:43 Lac Operon & IPTG Induction
7:43 Protein Analysis - SDS PAGE
8:01 Process Scale Up
8:08 Protein Characterization