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LC Troubleshooting-All of My Peaks are Tailing! What Should I Do?
Рет қаралды 15,072
Күн бұрынTailing peaks can be a problem when we are doing liquid chromatography (LC), and secondary silanol interactions are one of the causes. Here, using the example of basic drugs, we explain what causes them and cover a few steps that we can take to minimize the effect of these secondary silanols on our peak shape when we are troubleshooting.
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Additional Resources
All of My Peaks are Tailing! What Should I Do? | www.restek.com...
Troubleshooting HPLC - Tailing Peaks | www.restek.com...
When Should You Use a Buffer for HPLC? | www.restek.com...
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Restek Recommended Tools & Parts
Mobile Phase Accessories | www.restek.com...
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@amolgawande9248 4 жыл бұрын
Thank you much, usually it is difficult to understand but this animation makes eases an understanding of secondary interactions ...I appreciate, ty
@RestekCorp 4 жыл бұрын
You're very welcome!
@user-dm9km2hg4c 6 жыл бұрын
I see this a Reverse phase column and your structure is only the residual silanol group on it only. Thank you for this. Very informative.
@137bob3d 3 жыл бұрын
super idea these 'tips' vid's ... my interest was seeing if flow rate excess played a role too w/ tailings
@victorboyko6178 3 жыл бұрын
Hello. At 2:29 the bottles are closed with white caps to prevent loss of solvent. Can you tell me how they are called correctly?
@RestekCorp 3 жыл бұрын
We call them Hub-Caps, though other companies may have different names for them. We made a short video about them kzfaq.info/get/bejne/acCarNCn3NLRenU.html and you can find them on our site www.restek.com/en/products/accessories-labware/lc-accessories/Mobile-Phase-Management/7257/
@sachinpandit7786 2 жыл бұрын
But whan triethylamine added in mobile phase found result reduce the tailing while triethylamine have amine group why not show secondary interaction
@raminniroumand2336 6 жыл бұрын
what about fronting ??? i have trouble with that
@user-dm9km2hg4c 6 жыл бұрын
This is not a c18 Column! Is this a special column?
@AlphaNumeric123 Жыл бұрын
Are there any issues with buffers corroding the pump pistons? Does ammonium formate specifically cause these issues? I know certain salts can be quite problematic, but I’m not sure about ammonium formate specifically.