Reverse Transcriptase PCR ll RT PCR II

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Reverse Transcriptase PCR ll RT PCR II

Рет қаралды 6,400

Күн бұрын

The video contains a detailed discussion of Reverse transcriptase PCR.
Reverse transcriptase polymerase chain reaction (RT-PCR) is a modified PCR-based amplification technique that uses RNA, rather than DNA, as the initial template.
Reverse transcriptase is an RNA-dependent DNA polymerase, catalyzing DNA synthesis using RNA as the template. The end product is known as complementary DNA (cDNA). cDNA is not subject to RNase degradation, making it more stable than RNA.
In RT-PCR, the starting RNA is subsequently degraded, dsDNA is produced, and PCR amplification proceeds in the usual manner.
#pcr #rtpcr #csirnet #bsc #neet

Пікірлер: 26

@15432WQsshdjfh--....... 7 ай бұрын

Wow great 👍mam

@adreejaaa 3 ай бұрын

amazing explaination ma'am!!!

@ummeammarah9351 5 ай бұрын

Very well explained thnku❤

@alwayslearning3.52 4 ай бұрын

Crystal clear ❤❤❤

@eshaahmed8489 Жыл бұрын

Bestest vedio ever..... I have prepared my presentation from this vedio very easily❤

@motivator014 Жыл бұрын

बहुत ही शानदार 👌👌👌शब्द नही है आपकी प्रशंषा करने के 🌺🌺🌺

@kalyanlohar476 Жыл бұрын

Thank you maam.... Very nice informative, Excellent... Lecture 🙂🤗👍👍

@piyaaaa077 Жыл бұрын

Hats off to you mam..what a great explanation.. I'll never forget RT PCR afterwards...thank u so much

@AdityaRaj-zk9eh 7 ай бұрын

thanks mam

@ankitabiology 7 ай бұрын

welcome

@user-iw5ik1jd5u 7 ай бұрын

Best explanation ever♥️😍

@youtubeIslamic. Жыл бұрын

MashAllah 💖 ma'am bhut acha way ha thanks

@avi4707 9 ай бұрын

Superb lecture mam ❤

@kailashvijay6809 Жыл бұрын

बहुत सुन्दर एक्सप्रेशन।

@shuvosarkar726 Жыл бұрын

Very Nice Presentation

@choureyharshi2268 10 ай бұрын

Tq so much.. very helpful

@Innerability 7 ай бұрын

Nice

@aquabitelaxmiprasad 8 ай бұрын

Very nice explanation madam

@yougalpratapsingh2759 Жыл бұрын

Best explanation

@user-jy9nn6gm1i 18 күн бұрын

Bhut achi video thi .... ❤❤❤ But video quality achi nehi thi ...Kiya likha hova hi sahi sy samjh nehi a rha tha .... But Excellent effort😊😊😊😊

@hoorain-xr7vp 9 ай бұрын

Thank you so much mam

@shivabose1448 Жыл бұрын

Question: You are setting up a reverse transcription polymerase chain reaction (RT-PCR) experiment to amplify a part of the coding region of a gene using cDNA as the template. When you look at the results, you find that the PCR reaction fails to amplify any DNA. To identify the possible reasons for this result, you checked your lab notebook regarding the reagents you have used for RT-PCR. Which one of the options given below is likely to result in the absence of any amplification? (a)» (i) Reverse primer, nucleotide bases, and reverse transcriptase for the RT reaction; (ii) Forward and reverse primers, nucleotide bases, and Taq polymerase for the PCR (b)». (i) Forward primer, nucleotide bases, and reverse transcriptase for the RT reaction; (ii) Forward and reverse primers, nucleotide bases, and Taq polymerase for the PCR (c)». (i) Random primer, nucleotide bases, and reverse transcriptase for the RT reaction; (ii) Forward and reverse primers, nucleotide bases, and Taq polymerase for the PCR (d)». (i) oligo(dT) primer, nucleotide bases, and reverse transcriptase for the RT reaction; (ii) Forward and reverse primers, nucleotide bases, and Taq polymerase for the PCR