Reverse transcription PCR - RT PCR procedure

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Reverse transcription PCR - RT PCR procedure

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Күн бұрын

Reverse transcription PCR - This lecture explains about the principle of reverse transcriptase polymerase chain reaction also known as RT PCR. RT-PCR (reverse transcription-polymerase chain reaction) is the most sensitive technique for mRNA detection and quantitation. Watch this video lecture to understand the mechanism behind the reverse transcriptase PCR to amplify target RNA sequence by reverse transcribing it into complementary DNA.
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Thank you for watching the video lecture on Reverse transcription PCR .

Пікірлер: 274

@Gbemi78 4 жыл бұрын

Sir, you don't know how much your videos have helped a lot of people. you really don't know. I just appreciate your effort and God bless the womb that gave birth to you. Gracias

@shomusbiologyofficial 4 жыл бұрын

Thank you so much for appreciating my efforts. All the best for future career

@nailaperveen4113 4 жыл бұрын

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@SarojTamang-kv2ne 2 жыл бұрын

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@shomusbiologyofficial Жыл бұрын

Glad to hear that. Stay tuned

@reilian Жыл бұрын

same i swear to god his videos are life saver

@londonspade5896 Жыл бұрын

@@reilian 100% better than what university provided to learn with

@ousaxx 8 жыл бұрын

You have a video on everything! Such a boss, thank you! :D

@yumnafouzer5923 4 жыл бұрын

i'm on the verge of literally giving up on bio and then your videos are there. wow! thank you so much

@shomusbiologyofficial 4 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

@yumnafouzer5923 4 жыл бұрын

@@shomusbiologyofficial gladly doing that!

@vijayag8116 3 жыл бұрын

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@nasera9859 5 жыл бұрын

I am thrilled to bits that you are in KZfaq sharing easy to understand biology. Thumbs up buddy!

@shomusbiologyofficial 5 жыл бұрын

Glad to hear that

@Abhishek-ou1gc 4 жыл бұрын

Came here to understand RT-PCT techniques currently being used in Covid detection ❤️🔥🙏🏼

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@user-qi7qm4oy7z 3 жыл бұрын

No bro that is Real time PCR which is qRT-PCR used in covid19 detection

@seeramapoorva6792 3 жыл бұрын

Doing a Ph.D. related to molecular bio with zero background in Bio, your videos have and are helping me a lot. Can you please make a video on how to analyze sequencing data from NGS.

@shomusbiologyofficial 3 жыл бұрын

Glad to hear that you're getting benefit from my lectures

@misbahhussain1021 3 ай бұрын

U r the legend sir b4 8 years still perfectly u explained it ❤

@shomusbiologyofficial 3 ай бұрын

Thank you

@gayatrihaldar5570 2 жыл бұрын

Sir . You are literally a saviour 💕 may god bless you

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@gayatrihaldar5570 2 жыл бұрын

@@shomusbiologyofficial u deserve it all . And that's the least we can do . Even my teacher studied from your vdos. 😘💕

@ashishyt7953 2 жыл бұрын

Sir I was suffering with a confusion in this reverse transcription all thanks to you I got all my concepts cleared thankyou sir ❤️

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@aamirhussain. 2 жыл бұрын

love u sir.... helps a lot. lov from kashmir 💛

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@nissiravuri3063 Жыл бұрын

Excellent teacher for me ...forever sir,thank u so much sir...god bless you and ur future

@shomusbiologyofficial Жыл бұрын

Thank you so much for appreciating my efforts

@carolinejilbert2956 3 жыл бұрын

What would I do without you.... Thank you so much!

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

@tilakchandrakar6895 3 жыл бұрын

Thank you for excellent explanation of RT-PCR. You described it well and minutely differentiate the similarities and dissimilarity's between the similar term's.

@shomusbiologyofficial 3 жыл бұрын

Thank you so much for appreciating my efforts

@unliuniverse2999 4 жыл бұрын

Another nice video from you. Salute 🥰🥰👏👏

@shomusbiologyofficial 4 жыл бұрын

Thank you. Glad you liked my lectures

@vaishalisharma7773 3 жыл бұрын

thnk u again sir... cleared all the doubts regarding this topic

@rashmisarkar5289 5 жыл бұрын

So nicely explained sir...you are the best teacher forever....

@shomusbiologyofficial 5 жыл бұрын

Thank you. Glad you liked my lectures

@rashmisarkar5289 5 жыл бұрын

@@shomusbiologyofficial most welcome sir...

@nothingggg656 5 ай бұрын

Thankyou so much sir for beautiful explanation 😄💫

@shomusbiologyofficial 5 ай бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@meerpeya5139 6 жыл бұрын

Thank you so much.... sir you are awsyme ...the way of ur explanation. ..nice ..your viedo always help me..thanks once again I got 78% from ur viedos

@shomusbiologyofficial 6 жыл бұрын

That's so nice to hear. Congratulations. Please subscribe and share

@reginadarlong5655 Жыл бұрын

May God bless you sir

@shomusbiologyofficial Жыл бұрын

Thank you

@shahzadahmad9630 4 жыл бұрын

sir your video is very helped a lot of people, but prepeire new more advance and improve

@shomusbiologyofficial 4 жыл бұрын

New videos will come soon

@carolinakohl9543 3 жыл бұрын

Thanks for guiding me through medschool!!! You are awesome!!! 😊

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@syedaafsananushrat890 2 жыл бұрын

thanks a lot. the video is so helpful for me.

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@facsvlog 2 жыл бұрын

Each n every single words is matter means alot😍

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@shaarifaltaf6473 3 ай бұрын

U r great sir

@shomusbiologyofficial 3 ай бұрын

Thank you

@pampaniyaarashi6641 8 жыл бұрын

VERY NICE BROTHER ,ONE MEN ARMYYYYY.ALL THE BEST

@shomusbiologyofficial 8 жыл бұрын

+Arashi Pampaniya thank you. Glad you liked my lectures. Stay tuned

@openwrt5504 8 жыл бұрын

Like an expert. Great.

@rajwantkaur7019 4 жыл бұрын

sir your way of teaching is excellent 👏

@shomusbiologyofficial 4 жыл бұрын

Thank you so much for appreciating my efforts

@otgonsurenmunkh-ochir1202 4 жыл бұрын

you are really brilliant teacher i’ve ever seen 😇

@AmritKumar-vr7vn 3 жыл бұрын

Beautifully Explained!!

@shomusbiologyofficial 3 жыл бұрын

Thank you

@iloveegypt1324 8 жыл бұрын

Excellent ... very beneficial and simple thank you very much for all your unique videos, I appreciate all of them.

@MrDeep5481 3 жыл бұрын

Content is good , voice is not loud

@professorsanamalik1650 7 жыл бұрын

sir excellent way of explanation.i must acknowledge:)

@krishnenasu7110 3 жыл бұрын

Thank you its a very nice video

@shomusbiologyofficial 3 жыл бұрын

You're welcome

@shaghayeghsaeidi7159 Жыл бұрын

You are amazing ❤

@shomusbiologyofficial Жыл бұрын

Thank you

@sahewa100 6 жыл бұрын

Really valuable information sir....go on...all the best !

@jacksonwestaway3376 3 жыл бұрын

Shomu Bro you are a legend

@yogaranisivapalan5253 3 жыл бұрын

Thank you so much sir. I'm very grateful to you my lifetime.

@shomusbiologyofficial 3 жыл бұрын

Thank you so much for appreciating my efforts

@brakstrongarm9551 2 жыл бұрын

Another great video. Thanks. I am about to start doing RNA purification and cDNA creation so good to get a theory primer first:)

@shomusbiologyofficial 2 жыл бұрын

Thank you

@Hanan-wq1op 3 жыл бұрын

Thank you for explaining

@shomusbiologyofficial 3 жыл бұрын

You're welcome

@abdulsaif1607 8 жыл бұрын

sir,, can u please post a lesson on Upstream and downstream processing..

@dhbtna 7 жыл бұрын

Very good explanation as other videos of yours. I like board explanation more than the animations.

@santhoshtrashers9536 2 жыл бұрын

Good sir. Please do video on Molecular markers and QTL mapping

@shomusbiologyofficial 2 жыл бұрын

Sure

@judithjara001 8 жыл бұрын

excellent explanation. . super clear ... thank you so much!!

@iqrabatool2739 4 жыл бұрын

Really helpful

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@iqrabatool2739 4 жыл бұрын

@@shomusbiologyofficial I want to ask a question to you about my study method and my concepts. I am a biotechnology student. Can you please help me?

@jkssbexamupdate 6 жыл бұрын

Sir u r explaining things very well But u r getting more faster Sir if u will teach slowly I think the things will be explained more.Although ur way of teaching is best

@ismaqaiser9431 5 жыл бұрын

Superb explanation....Thanks for giving us such a better understanding regarding RT PCR.

@soumyaojha3537 Жыл бұрын

very informative lecture...😁

@shomusbiologyofficial Жыл бұрын

Thank you

@aliviadalal726 3 жыл бұрын

Well explained sir..Thank you so much

@shomusbiologyofficial 3 жыл бұрын

Thank you

@amicablemisbah6360 6 жыл бұрын

just wow sir!!! I follow you lectures...

@alien3143 3 жыл бұрын

U n we don't know whn u mke this video.that each person in this World will spell this Word😂

@shomusbiologyofficial 3 жыл бұрын

Yes

@apekshadandi7767 5 ай бұрын

Thank you 🙏

@shomusbiologyofficial 5 ай бұрын

You're welcome

@shronitgaur1085 2 жыл бұрын

Thanks bro help...in genetic engineer exam

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@elegansist 8 жыл бұрын

Well done! This is well and simply explained.

@Student_2324 Жыл бұрын

I am in class 11 and we are making a model of rtpcr in sci exibition , What is the function of Cdna ?? Not understood

@alabaadewoleadebayo7703 6 жыл бұрын

Thank you more Sir... I do watch and like your videos.

@shomusbiologyofficial 6 жыл бұрын

+Adebayo Alaba thank you. Glad you liked my lectures

@tainusali4165 5 жыл бұрын

Thanks sir for your tremendous lecture about RT-PCR.

@shomusbiologyofficial 5 жыл бұрын

Thank you. Glad you liked my lectures

@kamalmurtala1368 4 жыл бұрын

Kamal from Nigeria, excellent

@kashifahmad8250 8 жыл бұрын

you have done a great job brother, simple and easy explained

@shomusbiologyofficial 8 жыл бұрын

+kashif ahmad thank you very much

@humanoid1960 8 жыл бұрын

mate you are great!!!!

@antmes5304 3 жыл бұрын

Well done ! Clearly explained ! great job

@shomusbiologyofficial 3 жыл бұрын

Thank you so much for appreciating my efforts

@geekymedico.8099 3 жыл бұрын

Thank u soo much sir... .. U r lectures help a lot...,🤗🤗🤗🤗🤗🤗🙏🙏🙏🙏🙏

@gajananawachar6548 4 жыл бұрын

Nice sir

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@Abdulsalam-ln4pv 8 жыл бұрын

clarity is Awesome

@saichandramacherla7661 7 жыл бұрын

explained is outstanding

@lebronprasad2632 3 жыл бұрын

Can u provide video on mini preparations and purification of plasmid dna

@Jumpingfrog1234 8 жыл бұрын

Great video. Well explained sir.

@deeksharai4212 5 жыл бұрын

Sir I am following ur almost all videos.. U r giving good explanations bt ur videos are too much long.. Please make clear cut explanation.. Thank j

@shomusbiologyofficial 5 жыл бұрын

Thank you. Glad you liked my lectures

@aboutstudies123 6 жыл бұрын

Thank you very much Sir. It is really helpful👌👌

@shomusbiologyofficial 6 жыл бұрын

Glad you liked it

@danielvizcaino5970 8 жыл бұрын

Thanks! Great job explaining

@krishnabaruah7185 6 жыл бұрын

Grt learning..thnku sir

@shummu1458 6 жыл бұрын

very great explanation sir👍

@klam77 4 жыл бұрын

Nicely Explained!

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@saminaahmed934 5 жыл бұрын

Thank u so much sir, it is much beneficial nd i do watch ur most of the video, it does help me a lot..!!🤗

@user-mn8gv6up9b 5 жыл бұрын

it was rly helpful. Thank you so much

@Yusrrabibi Жыл бұрын

its good

@shomusbiologyofficial Жыл бұрын

Thank you

@sujatamishra1304 3 жыл бұрын

Please make a video on 5' and 3' RACE

@simplescience3959 7 жыл бұрын

Can someone explain the difference between the Random primer and oligodT because he said both of them can bind to any place and start replication.

@shridharhiremath2896 7 жыл бұрын

No the oligo dT primers will bind to the poly A tail ,they are not random.....whereas the random primers bind to the random sequence of the m RNA

@physicsiseasy2403 3 жыл бұрын

Covid Era me apka video dekh rhe h .

@sanskardauneria1070 6 жыл бұрын

Sir question**there is uracil in place of thymine as a base pair in RNA but in DNA we have thymine so from where did the thymine come from two RNA that have uracil in reverse transcript ion

@lawinahmedomar1108 3 жыл бұрын

thank you so much

@newboyshostel 3 жыл бұрын

Sir, can you make video on Polymerase Spiral Reaction (PSR) or LAMP PCR..? As there is no video on this topic on you tube and I hope I will get on this platform only . Regards.

@pampaniyaarashi6641 8 жыл бұрын

GOOD JOB,,,,,,THANKS

@mohitsingh-ev8nm 7 жыл бұрын

really valuable information sir....go on...all the best I listen more to Ur lecture s dan our college classes...

@shomusbiologyofficial 7 жыл бұрын

+mohit singh thank you. Glad you liked my lectures and

@TownHeroine 7 жыл бұрын

Thank you

@shomusbiologyofficial 7 жыл бұрын

+TownHeroine you're welcome

@shankarkumarjha2320 4 жыл бұрын

Thank you sir

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@iamcoolerthancool 4 жыл бұрын

Thanks for making this video! You’re a great teacher!!! 👏🏼👏🏼👏🏼

@shomusbiologyofficial 4 жыл бұрын

Glad to hear that you're getting benefit from my lectures

@vivekyadavkm5307 3 жыл бұрын

RT PCR is explained you sir 5 years ago it's and now people are tested

@agustin.8 6 жыл бұрын

Gracias. Muy bueno

@ShilpashreeGowdaRCZMI 3 жыл бұрын

Very nice sir but I am student 🥺 I get little complicated in assuming ur vedio was very good but little confusion in my mind 🙄

@bhargavatejasallapalli7648 2 жыл бұрын

Can you please add the sources/cite that use?

@shahinagoria2921 6 жыл бұрын

Useful video

@shomusbiologyofficial 6 жыл бұрын

You're welcome

@mohamedelgendy9629 4 жыл бұрын

thank you

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@rohitkumar-et3np 7 жыл бұрын

very nice....

@mallikanagamanickam5535 5 жыл бұрын

Sir u mentioned tat rtase won't survive at higher temperature Tan 37 rite for linearising of rna we r heating it to 70 so won't tat denature tere itself

@Biologybucks 6 жыл бұрын

Plz make video on fermentation kinetics and how to remember that

@Friend_forever_Pure 3 жыл бұрын

Sir is RT-PCR , reverse transciptase PCR and qPCR is same or different?

@radhakrishna4329 3 жыл бұрын

sir can you explain a little bit about detection of pathogen by pcr please ?? its a earnest request

@roseb2105 6 жыл бұрын

why cant one just use 37 degree temperature even with multiple repeats when pcr process happens doesnt it act on ( multiple strands and sequences at the same time i dont understand the thing about higher temperatures with repeats?

@minhanadim7571 2 жыл бұрын

Is there any video lecture/demonstration available on "Nucleic Acid Sequence-based Amplification"(NASBA) over your channel because I couldn't find it? If you've not, then pls it's a request to make one lecture on it.