The principle of Real Time PCR, Reverse Transcription, quantitative rt-PCR

Рет қаралды 666,713

Күн бұрын

This video is an easy and full explanation about the principle of real time PCR.
For better understanding watch the previous video about the principle of PCR:
• The principle of PCR-P...

Пікірлер: 533

@sherylrodriguez2574 5 жыл бұрын

Good video! just a quick comment to clarify that RT-PCR is Reverse Transcription PCR and Real Time PCR is Q-PCR, for Quantitative PCR, to avoid confusion. Thanks for your video! It was helpful

@Biomeducated 4 жыл бұрын

Her videos are awesome! That's the reason I've put her in my '10 Biomedical Science Channels to watch' video on my own channel! Just a pity she doesn't upload anymore :( Hope for a revival!

@aparnasrivastava8064 4 жыл бұрын

Good explanation

@dranshumansahu6114 3 жыл бұрын

Biomed Master yeah but small blunders.....she need to improve that......and she’ll become best

@mrlawilliamsukwarmachine4904 3 жыл бұрын

Intetesting!

@rickscheck5330 3 жыл бұрын

" RT-PCR is Reverse Transcription PCR" That's what I thought. She says "Real Time" So what's the difference?

@reindorfboateng7230 3 жыл бұрын

This 5 years ago video has provided me with great knowledge and insight. Thank you very much for the clear and detailed explanation

@manuelmendez9372 6 жыл бұрын

I came here crying due to I couldn't understand my class today. I left loving RT PCR. Amazing work! Thank you so so muuuuch!

@biomedicalandbiologicalsci4989 6 жыл бұрын

Thank you for your comment, it makes me happy o hear that :)

@Calbishi1 5 жыл бұрын

Yes, Manuel, very handy and great video! Will help our QFT team for sure

@Plantshealth 5 жыл бұрын

@@biomedicalandbiologicalsci4989 Egyptians are making good videos.

@jsingh265 3 жыл бұрын

@@biomedicalandbiologicalsci4989 amazing. Your videos still having big impact.

@danial17011 4 жыл бұрын

I have to admit, pretty impressive ability to comunicate, you really made it cristal clear despite english not being your first language. Thank you very much!

@Zweileiter 5 жыл бұрын

great video! I already have a Master in Biologie, but never was interested so much in molecular biologie and I totaly get everything about qPCR now! Thanks!!!

@dryounisrather7855 2 жыл бұрын

i am not a molecular biologist but i can follow and understand your lecture easily, now i can interpret the COVID19 RT-PCR diagnostic result just looking at the curves and figuring out CT value quite easily, thanks and keep sharing knowledge

@yashkarmarkar8056 4 жыл бұрын

This is the most logical progression for someone who's looking to understand PCR. Really well structured content, big up!

@DM-xq3qg 6 жыл бұрын

your channel deserves more views! i could not have done well at university without you

@biomedicalandbiologicalsci4989 6 жыл бұрын

Thank youu .. this really makes me happy :)

@AqleemAbbas 3 жыл бұрын

@@biomedicalandbiologicalsci4989 Hello Madam, Why have you stopped making videos? Continue please

@stianborg-stoveland661 6 жыл бұрын

Thank you! I needed to refresh this topic, and you covered it all, good job!

@ilaydademir228 4 жыл бұрын

all mechanisms explained clearly! no insignificant details, just the main points, thank you : )

@onzimadaniel3747 Жыл бұрын

Haven't seen such a complete and educative video!!! Thank you.

@mebriezemene9250 6 жыл бұрын

Thank you very much It is a nice video with a great explanation. It is helpful to me

@enricocalvanese8223 6 жыл бұрын

I wish you had more views. You are such a good lecturer and I am really grateful for your time in uploading these videos!

@munerhd6095 3 жыл бұрын

Omg thank you soooooo much so much 😭 do you know how many times i tried to understand and looking for videos seeking god to understand this pain in the neck machine?!!! You’re awesome I finally understand it again thank you so much my savior 🥺💕

@antonioassis7981 6 жыл бұрын

Seriously, your video is totally amazing. What you have shown here, I guarantee you, most people from the field doesn't know. Just one little insignificant correction, cDNA means "complementary DNA" and don't "circle DNA".

@mehwisha.salman5517 3 жыл бұрын

She said cyclic not circle

@stanleyho8009 2 жыл бұрын

@@mehwisha.salman5517 still wrong

@stanleyho8009 2 жыл бұрын

I’m actually surprised… I’m first year university student and following the course biotechnology and society in the Netherlands and this came by real fast that I forgot exactly what is was so I looked it up again. I cannot imagine people in the field at least a lot of them not knowing about this if they specialize in this field,

@irinasegovia4900 5 жыл бұрын

Clear and simple explanation. Thank you and keep up the great videos.

@austinmiles5818 6 жыл бұрын

This is absolutely incredible. Just what I needed for a presentation next week.

@biomedicalandbiologicalsci4989 6 жыл бұрын

HAHAHA, very good. Hope you put my channel as a reference :p

@VLAREZENDE1983 4 жыл бұрын

Biomedical and Biological Sciences I hope too! Very good informations in you video!

@monaalbalawi6470 2 жыл бұрын

@@biomedicalandbiologicalsci4989 can i have this presentation as a file?

@audreyvanya5520 4 жыл бұрын

Thank you for such a simple and clear explanation between these two assays.

@abhayiyer8864 5 жыл бұрын

U explain well. I didn't understand RT PCR for my course. Now I understand it. U have great teaching talent.

@anthonyvillarama6806 5 жыл бұрын

Awesome. Your presentation and explanation are clear. The best lecture out there. Keep your lectures coming.

@farahdagane157 6 жыл бұрын

Another wonderful, informative and concise video, as always. Keep going

@benjaminsulemankurya6664 3 жыл бұрын

You are an excellent tutor, very clear message. I learnt things I could not understand from other videos.

@Tony_Ss-bx2dp 4 жыл бұрын

It is an amazing video! thanks a lot for this explanation. Just one correction; cDNA is for "complementary" not for "cyclic" (22:04). Great job!

@Gigis1111 3 жыл бұрын

Well done. The best explanation I could find!

@AhmedSaad-om9vf 4 жыл бұрын

Thank you for this amazing video , really i gain great knowledge about qpcr this due to you amazing lecture , i am looking forward to more lessons

@oluwashinaabiodun3667 6 жыл бұрын

well explained in the simplest form ever! Kudos

@nandinipriyathangavel8293 4 жыл бұрын

Thank you madam for your awesome explanation....first I didn't understand the rt PCR before now I'm clear in this topic ...once again thank you madam

@sagarvemula6800 5 жыл бұрын

Your step by step explanation is excellent Thank you

@moazal-ghamdi9017 5 жыл бұрын

Thank you so much. The presentation was so clear. Keep up the good work.

@PoseidonXIII Жыл бұрын

This helped it finally click for me! Much appreciated.

@adlesal24 Жыл бұрын

You are magnificent. You do not leave any point without detailed explanation. God blessings ❤

@farshidyekani3957 3 жыл бұрын

Good presentation .. Just an important point: cDNA is Not cyclic DNA, It's Called Complementary DNA.

@adityatayde98 3 жыл бұрын

Thanks for correction

@boinualizeh4877 3 жыл бұрын

Yes👍

@archanar9676 2 жыл бұрын

Good presentation

@naveedahmed8544 2 жыл бұрын

@@boinualizeh4877 good

@educationinfo5080 Жыл бұрын

Means this cDNA undergoes this cyclic reaction so one can say.

@sihaogao661 5 жыл бұрын

Thank you. Your explanation is very clear and the slides you made are awesome.

@JQPOINT 4 жыл бұрын

Your explanation is so succinct and understandable even for a non-scientist like me!

@fauziaazzahra8578 4 жыл бұрын

Thank you so much, it helps me alot to understand about pcr.. You explained it well so it easier to understand 👍

@miangulhilal6858 6 жыл бұрын

Outstanding job very informative and easy explanation of difficult thoughts many thanks for your knowledge sharing

@Analysis-Zone 2 жыл бұрын

This what i am looking for. Thank you for your effort and nice presentation...

@nagarajukanakavalli8465 5 жыл бұрын

Thank you so much.. I did cDNA synthesis next rt-pcr it is helpful for me

@thummalaharish5588 4 жыл бұрын

Nice effort in giving information to the beginners and even the experts. Thanks for the video

@erfanhatami7040 5 жыл бұрын

this is the first time i got what rt-pcr is.thanks a lot

@qiwenli6657 6 жыл бұрын

Thank You! Excellent job!

@1stPrimadonna 2 жыл бұрын

Thank you very much for this video ! You explian very clear and easy to follow. Hope you keep doing this

@viniciusgoularte2729 5 жыл бұрын

Thanks for the video, really helpful!

@medicineman786 3 жыл бұрын

You are the best!

@mehwisha.salman5517 3 жыл бұрын

You are amazing it took me years to understand ft pcr. But you did it in few minutes

@abubakarsaidumshelia550 3 жыл бұрын

This is Apt and Well Explained. Many Thanks.

@user-wf5sn9vg9k 5 жыл бұрын

Excellent video. I really understand several information. Thanks

@aracelyabarcainostroza5190 7 жыл бұрын

Thank you so much!!! it´s a really good explanation :)

@biomedicalandbiologicalsci4989 7 жыл бұрын

Thank you for the nice comment :)

@drsaimairam15 7 жыл бұрын

no doubt its an excellent presentation about RT-PCR

@GamingAmbienceLive 3 жыл бұрын

Very good video you did an amazing job simple and doesn’t miss important details, but doesn’t go into an unnecessary amount of detail.

@carolinec.picoli6849 5 жыл бұрын

Congratulations on the video, I found it well didactic.

@isaacclark9886 3 жыл бұрын

Really awesome video. Super helpful in clarifying this concept for me. Thank you!

@diya4939 5 жыл бұрын

amazing video i saw in my life i appreciate u

@thestoner2870 2 жыл бұрын

props gehen raus für des geile Video feier i extrem

@manthansurkar471 3 жыл бұрын

Nicely Explained, thanks!!

@ponrajarajan 4 жыл бұрын

Clarity in ur presentation. I enjoyed it.

@angelpaulrodrigues6477 2 жыл бұрын

Thanks for the amazing presentation!

@yuxi422 2 жыл бұрын

Really good video! So clear!

@gilsanaraujo6298 4 жыл бұрын

maravilhoso! ajudou muito!!!

@mdshahidulislam6805 5 жыл бұрын

Really a nice demonstration. Thanks.

@sametcirik4278 3 жыл бұрын

Finally I could understand the key information thanks to this video after days of another mixed incomprehensible video. Please keep doing this. Congr. 👏👏👏

@swedd2 3 жыл бұрын

This is such an informative video! Very useful for a graduate course I'm considering reattempting. Cheers

@mimalam5315 5 жыл бұрын

you explained it so well..thank you

@bryanjones7126 4 жыл бұрын

Excellent job! Thank you!

@enes_rafel 2 жыл бұрын

Thank you, perfect explanation!

@MohammedAmodu 5 жыл бұрын

Really helpful. Thank for your time

@sikchamomile 2 жыл бұрын

This video clarified a lot.. Thanks 🤩

@ursilayaseen2136 5 жыл бұрын

This explaination is very clear.thank u

@ahmednaji1701 6 жыл бұрын

It was really wonderful video. Many Thanks

@bonkap7131 4 жыл бұрын

Superb explanation. Thanks!

@usmanarshad1246 2 жыл бұрын

Thank you so much! It was wonderful explanation.

@sumitaganguly2081 6 жыл бұрын

Very well explained, thank you for the video:)

@drashnatmou862 3 жыл бұрын

Really helpful for refreshing the whole thing... Thank u so much...

@cyrtcheu2052 3 жыл бұрын

This video is very good, very absorbing, you explained this methods simply and precisely. The only thing I can say is that SYBR green instead of Cyber green. It's very interesting.

@muhammadhafizsulaiman7163 3 жыл бұрын

You explain it well friend. Thank you so much.

@yuromi 5 жыл бұрын

Thank you so much. it is very useful for me and very well explanation

@rubaithasan8911 5 жыл бұрын

EXCELLENT .......... very easy to understand..........good job

@rashadmunir112 4 жыл бұрын

Simply, God bless you

@mahdisalh7179 4 жыл бұрын

Thank you very much Today I understood real time PCR ( nice and simplified explanation) thank you so much

@sharbaridas4912 5 жыл бұрын

I have a presentation on RT-QPCR next week. Thanks so much.

@adillatullathiifatunjannah4937 4 жыл бұрын

Thank you So much, this is very helpfull

@dalyaneldaly5167 5 жыл бұрын

Really good job. Thanks very much.

@amila_desilva 4 жыл бұрын

Needed to refresh , thanks and very useful.

@noornoor-ic4rg 6 жыл бұрын

Great simplified explanation...Thank you

@biomedicalandbiologicalsci4989 6 жыл бұрын

welcome :)

@candid180degrees 3 жыл бұрын

It was very informative. Thank you!

@Sturdy_Penguin 5 жыл бұрын

This is such a helpful video. Thank you so much for it. The only thing I noticed, was the use of "probability" instead of "possibility" at 17:38, where "probability" means "very likely", in this context. English is weird, sometimes, I know. But right. Your video was extremely helpful and the concept of rtPCR is very interesting. I'm glad it's so simple, too, as I have to be able to do it really soon.

@SaraTahaFPTU 2 жыл бұрын

Thank you for the very informative video. Easy to understand and to listen to your English accent. and very great presentation skills. Thanks a lot

@shariquemf129 3 жыл бұрын

The way u try to make us understand is just lovely.. thanks for the video and explaination 🙏🙏❤️

@yatagan1 6 жыл бұрын

thank you very much. i like your explanation. best wishes from Turkey :)

@Musicwithsahan 4 жыл бұрын

Great explanations!

@victoriaaquilino3529 5 жыл бұрын

Awesome video. Thank you.

@pritichristian2673 2 жыл бұрын

Very detailed explanation and vedio. Thanks

@poojautekar1446 4 жыл бұрын

Great explanation... And significant points covered... Keep uploading such videos.. looking forward to it...

@Padmashrinaren 2 жыл бұрын

This was such a life saver 🤧🤧❤❤❤🤧🤧

@theivun 5 жыл бұрын

You are the Bioanalysis queen!

@julianomachado7050 3 жыл бұрын

Amazing explanation!

@MrRamaeri 3 жыл бұрын

Thank you very much. I needed to refresh this topic.

@noorulain7302 5 жыл бұрын

Good video really, love from Paklistan...........

@thehim5458 5 жыл бұрын

amazing explanation...keep going on...love and peace

@sozzand3058 3 жыл бұрын

You are so good...thank you for the useful video