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In this video we discuss Sandwich ELISA in Hindi, ELISA Basics and ELISA Test procedure as well.
Sandwich ELISA typically require the use of matched antibody pairs, where each antibody is specific for a different, non-overlapping part (epitope) of the antigen molecule. A first antibody (known as capture antibody) is coated to the wells. The sample solution is then added to the well. A second antibody (known as detection antibody) follows this step in order to measure the concentration of the sample.
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