Sandwich ELISA | Basics Explained

No video

Sandwich ELISA | Basics Explained

Рет қаралды 82,403

Күн бұрын

In this video we discuss Sandwich ELISA in Hindi, ELISA Basics and ELISA Test procedure as well.
Sandwich ELISA typically require the use of matched antibody pairs, where each antibody is specific for a different, non-overlapping part (epitope) of the antigen molecule. A first antibody (known as capture antibody) is coated to the wells. The sample solution is then added to the well. A second antibody (known as detection antibody) follows this step in order to measure the concentration of the sample.
Hope you find this video helpful. In case you have any doubts please feel free to ask in comments.
I keep checking comments and reply to them.
Related Videos:
Competitive ELISA: • Competitive ELISA | Ba...
Indirect ELISA: • Indirect ELISA | Basic...
Direct ELISA: • Direct ELISA | Basics ...
🙋🏻‍♀️ 🛎Subscribe: www.youtube.co...
🗣 Telegram: t.me/baayo_cha...
👩🏻‍💼LinkedIn Page: / baayo
💁🏻 LinkedIn Profile: / artirani
🥳 Facebook Group: / baayo
👩🏻‍🎓Follow me on Unacademy: unacademy.com/...
#Sandwich #ELISA #Baayo

Пікірлер: 100

@BaaYo 6 ай бұрын

To access notes and practice questions, please join our Telegram channel t.me/baayo_official

@sarbaralisaha7344 2 жыл бұрын

Nice explanation. You have mentioned epitope simply as they are present at the surface of pathogen. That's for sure it's a part of antigen. But I think more precisely it should be defined as the part of a antigen that binds to paratope of an antibody. Paratope is the part of Fab region of antibody. More precisely the Complementarity Determining Regions i.e. CDRs. But yeah it's a very nice explanation.

@anjalithakur4439 4 ай бұрын

Your teaching skills is amazing no needs to read again 🤗

@suvadipjana1185 3 жыл бұрын

Arti mam, your reading skill are just amazing

@manjindersidhu7620 2 жыл бұрын

😇😇😍😍😍😍very very nice class ji…… thanks a lot 🥰🥰🥰

@panorama85 2 жыл бұрын

Vey Nice and detailed explanation. Thanks from Bangladesh 🇧🇩

@prashantchaturvedi6698 Жыл бұрын

Thank you Mam , my background not science but mai life sciences company me job karta hu bahot hi ache tarike se aap ne samjhaya thank you again

@dr.khushbugupta7626 11 ай бұрын

In which company you work??

@amitalenka1817 8 ай бұрын

Kya job hai apki??

@maahii8335 3 жыл бұрын

Thank u maam ☺️😊 plz maam also make a video on super critical fluid extraction technique

@shirishamishra3855 3 жыл бұрын

Thankuu mam bahut help hoti h apke videos se 😘😘😘😘😘😘😘😘

@madanmohan660 4 жыл бұрын

Explanation is very nice Ma'am.

@BaaYo 4 жыл бұрын

Thanks 😊

@hamzaanees7282 20 күн бұрын

thank you ma'am

@razorr1920 27 күн бұрын

Great mam. Can you do a video on EIS bio-sensing

@amitsinghbhadoriya6318 Жыл бұрын

Thanks 👍

@BaaYo Жыл бұрын

Welcome 👍

@KanuPriya-dn1ul Жыл бұрын

best way to explain..thanks mam

@AnshikaSingh-jh6mj 4 ай бұрын

Awesome lecture 👍👍

@pst_iit-delhi 4 жыл бұрын

Nice explanation ma'am. I have a question like for negative case you said that before adding substrate we do washing step but if the capture antibody and secondary antibody has bound to antigen but we don't know that as solution is transparent so at that time we also do washing or not like you didn't mentioned washing step in positive case before adding substrate like logically we should do washing. Can you please explain this?

@sunilmaahi 4 жыл бұрын

Thanx mam we always wait for ur video

@amitsarkar7215 2 жыл бұрын

Very very very nice

@manishshukla4503 4 жыл бұрын

Excellent. ....

@simransarkar4502 3 жыл бұрын

Mam I have a question, so what we can do is we can link enzyme with the capture Abs only why to complicate the whole process? Then also the color reaction can happen as enzyme will be linkedin to the capture antibody only.

@user-iq2sy3pn5u 11 ай бұрын

Maam can u plz provide videos explaing RIA and PCR ..as well as other topucs of biotech!! ..u teach very nice

@ashokmaich5258 2 жыл бұрын

Super explanation 👌

@mamtaom6098 4 жыл бұрын

Thank u mam.clear ho gya

@BaaYo 4 жыл бұрын

Thank you Mamta

@naveenappu5231 3 жыл бұрын

Tq mam for ur brife explanation

@livebiochemistry 4 жыл бұрын

Perfect explaination #livebiochemistry

@RajeshKumar-ui7pe 4 жыл бұрын

Man western blotting pr bhi videos banana plz

@BaaYo 4 жыл бұрын

western blotting per already video hai...search in playlist

@crenapun57 3 жыл бұрын

@AdityaRaj-zk9eh 5 ай бұрын

thanks mam

@pinkishekhawat8312 4 жыл бұрын

Ma'am just spose yadi corona ke antigen ko detect krna h but eske lia to capture antibody nhi h to hum elisa tech.use nhi kar skte

@BaaYo 4 жыл бұрын

We can use direct elisa

@pinkishekhawat8312 4 жыл бұрын

@@BaaYo tq ma'am

@Mky004 4 жыл бұрын

Plz make a video on competitive elisa.

@neetuyadav2881 4 жыл бұрын

So amazing vedio mam

@madanmohan660 4 жыл бұрын

Ma'am ek comparative video Bana dijiye achha rahega

@BaaYo 4 жыл бұрын

Sure, will try to make it.

@rashidchak8945 3 жыл бұрын

excellent

@rushisuthar18 3 жыл бұрын

Very nice mem 👍

@poonamsaini7735 4 жыл бұрын

Thanks man

@Amitsharma-lm7ss 4 жыл бұрын

Nice explained mam👌

@pavanghunawat4419 3 жыл бұрын

Mam supposed antigen have only one binding site means single epitope then how other antibody can bind to that antigen?

@shahirasadaf2665 Жыл бұрын

Generally an antigen has several or many different epitopes

@Ishika976 3 жыл бұрын

Indirect sandwich elisa can use detection of both antigen & antibody like in elisa hiv 4tg gen kit

@Unique_Science_Academy 4 жыл бұрын

Thank you mam 👍👍👍

@zahidaessa2336 Жыл бұрын

Ma'am application of ELISA explain kar de kindly.

@dakshapatel2455 4 жыл бұрын

Can u plzz upload video related to ...how ELISA technique is used to find plant disease with example

@prakashpandey6977 4 жыл бұрын

Light data analysis jo ki direct indirect and sandwich ka hota h apne bola tha end me bataogi aap...but end me ni h ye topic

@naurishansari8137 3 жыл бұрын

Thnks alot mam 🙏🙏🙏

@exmuslimadamsiker 4 жыл бұрын

Thank you mam a very wonderful lecture and tell me where the video of competitive elisa

@Megha-ij8eo 3 жыл бұрын

Maam ...aap "RIA" k bare mein video nahi banaya hai kya?

@knowledgejunction1643 4 жыл бұрын

Mam pls make a vdo that how can we calcut bacterial growth genecn tm

@mukulchauhan5379 3 жыл бұрын

Thanks mam

@bhanupratapsinghchauhan7798 8 ай бұрын

Can you make video on reverse ELISA

@blinders9088 3 жыл бұрын

♥️madam.....but I have a doubt...in sandwich elisa.the E-linked antibody bind to primary. Antibody not dorctly to the antigen

@rajthacker8329 2 жыл бұрын

If once the antigen is bind to the the capture antibody why we add detection antibody?

@ashuyadav1542 4 жыл бұрын

Hlo diii i need help from you ...actually i am biotechnology 2nd year soo their are 2 subjects first is molecular biology and second is immunology or immunotechnology .....these 2 subject i didn't understand it ....plss make video on them ur suggest me someone channel on KZfaq or unacademy... and i am also following you on unacademy

@ashuyadav1542 4 жыл бұрын

Sry i forget to tell...that bsc biotehnology 2nd year

@BaaYo 4 жыл бұрын

I will try to cover..starting new video series

@ashuyadav1542 4 жыл бұрын

Thanku soo much dii

@JyotiYadav-fm7rf 4 жыл бұрын

Mam how can we know that which type of elisa has been performed

@pradhumnsharma292 3 жыл бұрын

Chutya

@nehasoni1133 2 жыл бұрын

Mam please provides notes also for sessional exams

@afsanaparveen25 4 жыл бұрын

Good morning ma'am, kindly make a video on- PhD or BEd? after post graduation.

@VijayShankar88879 3 жыл бұрын

Mam good morning Mere pass question papers hai Biotechnology ki Aap in topics PR video banaiye dijiye mam

@manaswidas586 4 жыл бұрын

Hello mam... In this process..wash with water or other ?

@BaaYo 4 жыл бұрын

PBS buffer

@risingmartialartist9633 3 жыл бұрын

You explained in previous video indirect alisa started from antigen coating..........then here you are saying use capture antibody......is it important ?

@sakshisb419 3 жыл бұрын

Ham wishing ke liye PBS buffer he kyu use krte hai

@annierafi5427 3 жыл бұрын

Mam then just symptoms s recognize ni kr skty bcz there was no antigen???

@jannatfatima4714 3 жыл бұрын

Mam what is difference between direct and sandwich ELISA? Beacuse both are used for detection of antigens

@dr.yasirnawaz5230 3 жыл бұрын

appreciable (y)

@sabirahmadansari2578 3 жыл бұрын

Not understood

@payelkarmakar1405 3 жыл бұрын

Mam why do we use capture antibodies ? Antigen toh enzyme linked detection antibody se bind karega hi baad mein.... So why capture antibody at first step? With respect, clear my doubt

@manishasingh3536 3 жыл бұрын

Ma'am indirect ELISA is used only for antibody detection or can be used for antogen detection as well ?????

@iqraminhas292 11 ай бұрын

What type of ELISA we will use if we want to determine the presence of apoptotic proteins???

@BaaYo 11 ай бұрын

Sandwich

@debjitpatra7329 11 ай бұрын

Mam Mac elisa keya hote hee??

@kalerutuja7288 2 жыл бұрын

Indirect aur sandwich Mai kya difference hai?

@divyanshunagar239 3 жыл бұрын

Radio immuno assay wali video ka link mil sakta h ky plz

@SabeekaSajjalag- 3 жыл бұрын

👍🏻👍🏻

@manineeshrivastava586 3 жыл бұрын

Ma'am, could you please help me in finding biotech jobs in companies?

@anupammishra5443 3 жыл бұрын

😂😂😂😂😂

@anupammishra5443 3 жыл бұрын

Can upsc

@m.h.a5796 3 жыл бұрын

Ma'am why you not make new videos

@sonamsuhashini8110 4 жыл бұрын

In case of indirect detection of sandwich ELISA, the primary antibody used is derived from the patient or designed ?

@shivamdixit5860 3 жыл бұрын

The antibodies used in either direct & indirect sandwich method are genetically modified for particular antigen, Also this method is for antigen detection so only antigens are taken from patient's serum not antibodies.