Serial Dilution method | viable cell count method

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2 жыл бұрын

Serial dilution is a viable cell count method. In microbiology practical serial dilution method is used to count microorganisms present in any sample.
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Hi, I Am Rahul B Rathod, Welcome to Our KZfaq Channel RBR Life Science
About this video-
friends, In this video tutorial we are going to learn How to make serial dilution practical in the microbiology laboratory thank you so much...
Queries Solved:
1. How to make a serial dilution
2. How to perform serial dilution procedure in microbiology
3. How to calculate serial dilution
4. How to use serial dilution to isolate bacteria from soil
5. How to prepare a serial dilution
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#Viable cell count microbiology
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#english
#rbrlifescience

Пікірлер: 101

@simuchelomunkombwerkikbeiv6842 Жыл бұрын

Finally,well explained

@sandismike3822 4 ай бұрын

really helpful 👌 👏

@plantprotectionscience3646 2 ай бұрын

So nice

@deepakkumarpanda9350 Жыл бұрын

Informative Video with excellent explanation.

@rbrlifescience Жыл бұрын

Thank you Deepak..and stay tuned to watch such more informative videos... 👍😃

@dilawarkhanpathanniazi1369 Жыл бұрын

Sir very very good lacture for concept and understanding ☺️🤗 this method ♥️👌

@rbrlifescience Жыл бұрын

Thank you dilawar Khan ji...

@latestcraftwithrubab6393 2 жыл бұрын

Finally, find the authentic content

@rbrlifescience 2 жыл бұрын

Thanks for watching this video...Stay tuned to watch such more videos

@satyaprabhu6207 Жыл бұрын

K I got it!

@faith3193 Ай бұрын

Thank you very much sir for this great explanation ❤

@rbrlifescience Ай бұрын

Most welcome

@gangaarach3305 2 жыл бұрын

excellent explanation...thanks..

@rbrlifescience 2 жыл бұрын

Most welcome and stay tuned for more videos

@simantikasadhukhan6522 Жыл бұрын

Best video on CFU❤

@rbrlifescience Жыл бұрын

Thank You. ....and please suggest any topics on which I should make videos

@madhusahu3655 Жыл бұрын

Very helpful vedio thank you

@rbrlifescience Жыл бұрын

Thank you madhu....let me know other topics on which you want such videos

@asifk8459 Жыл бұрын

Sir, we say thank u so much sir.i require like this teaching sir . 😊🙏🙏🙏🙏🙏🙏🙏

@rbrlifescience Жыл бұрын

Thank you and please suggest such topics on which I should make videos

@harshadaadgulkar1029 Жыл бұрын

Nice explanation sir thank you sir for wonder full explanation

@rbrlifescience Жыл бұрын

Thanks harshada

@Sarojkumar-oq4hn 8 ай бұрын

Realy so nice explanation 😮

@rbrlifescience 7 ай бұрын

Glad you liked it

@lexusl849 5 ай бұрын

Great knowledge

@rbrlifescience 4 ай бұрын

Thank You

@malathibalu7467 Жыл бұрын

Very good explanation sir

@rbrlifescience Жыл бұрын

Thank you madam and please suggest any topics on which I should make videos

@gamecaptain1632 Жыл бұрын

Very helpful videos .... please make video on molecular technics ...PCR , RT-PCR, Nano drops , Sequencing, Electrophoresis,ELISA, Chromatography, spectrophotometry

@rbrlifescience Жыл бұрын

Thank you for suggestions...I will definitely make videos on molecular techniques

@abdulmajidal-yafei2793 2 жыл бұрын

thank you

@rbrlifescience 2 жыл бұрын

most welcome

@sandip45882 5 ай бұрын

Perfect 👍

@rbrlifescience 5 ай бұрын

Thanks 👍

@rafiqulislam3060 2 жыл бұрын

Thanks

@rbrlifescience 2 жыл бұрын

Welcome

@umamishra7757 10 ай бұрын

No words😊😊😊

@arshiaamjad9220 Жыл бұрын

very nice

@rbrlifescience Жыл бұрын

Thank you

@benibuntu2052 3 ай бұрын

❤❤❤ ⚘️🌷⚘️...Thank you...🙏🙏😊😊😊👍👍👍

@rbrlifescience 3 ай бұрын

Most welcome

@deepssih7787 Жыл бұрын

Thankyou so much sir for making my concept very clear...

@rbrlifescience Жыл бұрын

Most welcome Deep ...Please also suggest some concepts on which I should make videos

@fidanfazilova3542 14 күн бұрын

Thank you. It was very educative. One question. Why would we need to find out the number of cells? In what research do we need it?

@rbrlifescience 13 күн бұрын

To see the growth pattern of bacteria in culture we need to count cells.

@aerospaceplayer5811 Жыл бұрын

Good sir from pakistan

@rbrlifescience Жыл бұрын

Most Welcome

@salettedepintosouza5455 Жыл бұрын

if your trying to determine the bacterial resistance to a particular antibiotic using serial dilution and total viable count what are the acceptable counts and what are not the acceptable counts to say whether or not bacterial resistance is taking place?

@rbrlifescience Жыл бұрын

Please go through this wic.oregonstate.edu/microbiology-writing-guide-presenting-data

@aliyuabubakar-kt9wf Жыл бұрын

Pls sir do you have any video on complete method for culturing bacteria

@rbrlifescience Жыл бұрын

Please explain in detail

@dieudonnenyamaifofe9646 Жыл бұрын

What about if the 2 plates from 2 different dilution ( 10^2 and 10^3 for example) have colonies within the range of 30-300. What dilution should be consider to calculate the CFU/mL in the undiluted sample

@MorganHunte 10 ай бұрын

In theory - If you did a perfect serial dilution, the calculations that you do based on the colonies you've counted on these two plates should give you the same CFU/ mL. If the numbers are drastically different, there was likely an issue with the dilution scheme, with the calculations, or with the environment (i.e wrong with the agar). In the interest of time, I would choose to count the plate with fewer colonies. Hope this helps.

@rbrlifescience 10 ай бұрын

Yes thats very correct

@aitakil7294 Жыл бұрын

Après on fait la moyenne ?

@prathamkuril8277 Жыл бұрын

👋

@rbrlifescience Жыл бұрын

Welcome

@shaftasyed6244 5 ай бұрын

What about other plates in which we got countable growth how can we include those in calculation and we usually take duplicate or triplicate how can we calculate in such case

@rbrlifescience 5 ай бұрын

suppose you have plated 0.1 ml of sample of 10^3, 10^4, 10^5 dilution on three different plates. suppose you got 100 colony on 10^3 dilution, 11 colony on 10^4 dilution, 2 colony on 10^3 dilution, first conver the count cfu/ 0.1 ml to cfu/ml 10^3 (100 colonies) = 100 x 10^3 cfu/ 0.1 ml = 100 x 10^4 cfu/ ml 10^4, (11 colonies) = 11 x 10^4 cfu/ 0.1 ml = 11 x 10^5 cfu/ ml 10^5 (2 colonies) = 2 x 10^5 cfu/ 0.1 ml = 2 x 10^6 cfu/ ml now convert these 3 result to one specific dilution result 10^3 (100 colonies) = 100 x 10^4 cfu/ ml = 100 x 10^4 cfu/ml 10^4, (11 colonies) = 11 x 10^5 cfu/ ml = 110 x 10^4 cfu/ ml 10^5 (2 colonies) = 2 x 10^6 cfu/ ml = 200 x 10^4 cfu/ ml then average it (100+110+200)/3 = 136.66 x 10^4 cfu/ ml 136.66 x 10^4 cfu/ ml present in your original undiluted sample In the case of duplicate or triplicate samples, just average the results of specific dilution.

@iqrakhursheed7480 Жыл бұрын

Sir how to make different concentrations of the fungus

@rbrlifescience Жыл бұрын

As per my opinion fungal samples should be blended in specific broth medium before making dilutions. The procedure of serial dilution in similar as described in video.

@atiqaanis4098 4 ай бұрын

How can we will perform serial dilution method for tonsils sample after tonsillectomy for bacteria identification?

@rbrlifescience 4 ай бұрын

please refer medical procedure, I am explained only general procedure

@hamidahmadmalik572 Жыл бұрын

How to know the number of bacteria in original soil sample... We added 1 g in 50 ml... How to know the number of bacteria from that 1 g of soil ...

@rbrlifescience Жыл бұрын

Suppose you added 1 gm soil in 50 ml of Distilled water/ saline Now you are taking 1 ml of this sample and serially diluting it. You took 0.1 sample of each diluted tube and plated it. Suppose after plating 0.1 ml of sample of tube having dilution 10^-3 (ten power minus three ) you got 10 Colonies. Now it's 10 colonies in 0.1 ml of tube with dilution 10^-3 So convert it to ml 10 × 10= 100 × 10^3 cfu per ml So in 1 ml cfu count is = 100× 10^3 Multiply it by 50 = 100×50 × 10^3 = 5000×10^3 Cfu present in 1 gm of original soil sample Please watch my video on how to calculate CFU/ml

@sumitm8904 2 жыл бұрын

Can we use this method for counting the anaerobic bacteria ?

@rbrlifescience 2 жыл бұрын

You can make dilutions and then do pour plate method to count the anerobic bacteria. There are anaerobic jars in which you have to incubate plates.

@sumitm8904 2 жыл бұрын

@@rbrlifescience Thank for your explanation. But while performing dilutions there is a possibility that the anaerobic bacteria will come in contact with oxygen and the bacteria will die.So in order to protect anaerobic bacteria to get in contact with oxygen while dilutions, what precautions we have to take?

@rbrlifescience 2 жыл бұрын

@@sumitm8904 Dear sumit I totally agree with you. Generally any work on anaerobic bacteria should be carried out in anaerobic chamber. This chamber is purged with nitrogen and Carbon dioxide gas so all oxygen is removed from the chamber. Initially everything apparatus such as plate pouring, dilution making is done inside this chamber. and after plating plate is also placed in anaerobic CO2 incubator. It's little bit difficult to work with obligate anaerobic bacteria. There are different types of anaerobic media such as semisolid media which you can inoculate such bacteria.

@nithya1141 Жыл бұрын

Which tubes have present actinomycete, bacteria?

@rbrlifescience Жыл бұрын

It depends on samples

@aitakil7294 8 ай бұрын

290× 103 and 32×104 how To calculate them?

@rbrlifescience 8 ай бұрын

First convert 290x 10^3 = 29x10^4 Now take average of both 29x10^4 32x10^4 ------------------ Answer is 30.5 x 10^4

@pawar6444 2 жыл бұрын

Hello sir

@rbrlifescience 2 жыл бұрын

Hello, Good Morning

@aitakil7294 8 ай бұрын

How to do of average figures?

@rbrlifescience 8 ай бұрын

All dilution must be the same

@saurabhchoudhary3435 5 ай бұрын

Sir , is it true that single colony produce by single bacteria

@rbrlifescience 5 ай бұрын

a single bacteria will multiply a number of times and then only we are able to see a single colony. A single bacterial colony is made up of thousands of bacteria that arise by multiple cell division of single cell only.

@mitalibhoir6380 6 ай бұрын

Will this dilution technique useful for oil contaminated soil ?

@rbrlifescience 6 ай бұрын

Yes, off course but you have to choose correct medium of agar plates for plating as per type of microorganisms

@mitalibhoir6380 5 ай бұрын

@@rbrlifescience Yes!! we hv used Bushnell Hass agar as a selective medium for the isolation of hydrocarbon degrading bacteria. We did ur dilution technique. Thank you 💯🙏👍

@rbrlifescience 5 ай бұрын

Most welcome mitali @@mitalibhoir6380

@BritishBeachcomber 5 ай бұрын

That's how homeopathy works. Or doesn't.

@mishraratnesh6426 2 жыл бұрын

Sir mai aap se kuchh puchna chahta hu plzzz reply dijiye

@rbrlifescience 2 жыл бұрын

yes bro

@mishraratnesh6426 2 жыл бұрын

@@rbrlifescience Sir maine BSc (BZC) se ki hai , mujhe ,Media preparation, Autoclave operation, Watter sampling and testing, MLT testing ati hai kya mai microbiologist ban sakta hu plzz btaye ,ya apna no. Share kar dijiye taki me apse kuchh puchh saku

@rbrlifescience 2 жыл бұрын

@@mishraratnesh6426 Dear Ratnesh if you are a life science student then you can do the job of microbiology. If you have good knowledge of microbiology then there is no problem of getting job in the field in the microbiology. I am postgraduate in Zoology but right now i am working in microbiology field. So cheers.

@mishraratnesh6426 2 жыл бұрын

Sir ap contact no denge kya ?

@rbrlifescience 2 жыл бұрын

@@mishraratnesh6426 yes you can call me 9673690563 either on Saturday and Sunday

@sneha-zb1yg Жыл бұрын

32×103 should be divided by 10 not multiplied by it..I guess

@rbrlifescience Жыл бұрын

Dear Sneha initiatilly we calculated how much bacteria we got in 0.1 ml of sample. for plating we generally take out 0.1 ml of sample. once you calculate how many cfu in 0.1 ml of sample then we are simply converting it into 1 ml. multiply (0.1 ml X 10 )= 1 ml thats why we need to multiply cfu present in 0.1 ml with 10 and we will get cfu/ml.

@sneha-zb1yg Жыл бұрын

@@rbrlifescience thank you so much for clarifying..

@rbrlifescience Жыл бұрын

@@sneha-zb1yg most welcome and please suggest any topic or concept on which video is required.

@sneha-zb1yg Жыл бұрын

@@rbrlifescience if possible please make vedio on plaque assay, and protein-protein interaction techniques..

@akashrathod6065 Жыл бұрын

Rahul sir no send kara

@rbrlifescience Жыл бұрын

सुरक्षेच्या कारणास्तव मी तुम्हाला माझा मोबाईल नंबर देऊ शकत नाही जर तुम्हाला काही सहकार्य हवे असल्यास तुम्ही कमेंट करू शकता आणि मी त्याचा रिप्लाय तुम्हाला देईन

@akashrathod6065 Жыл бұрын

Ok sir

@rbrlifescience Жыл бұрын

May I know which subject or degree you are studying?