This video is a recording of a webinar presented by Oona McPolin of Mourne Training Services Ltd on the 4th August 2020. The webinar explored the different strategies, and combinations of strategies, which may be applied during HPLC method development. The best approach may vary depending on the particular requirements for a given separation.
Mourne Training Services Ltd provide a range of courses on the topic of HPLC, visit the website for more information: mournetrainingservices.com/co...
Navigation:
0:00 Introduction
0:15 Webinar info
3:42 Who's attending this webinar?
5:56 Challenges in HPLC Method Development
7:37 One size fits all?
8:02 Choice of strategy depends on
9:27 Is your desired method...
15:21 What is your greatest resource challenge?
16:43 2 Phases of method development
17:54 Examples of strategies
19:47 Quality by Design (QbD)
20:42 Analytical Quality by Design (AQbD)
21:50 Find a method in the literature
22:55 Pros and cons
27:09 Trial and error
29:23 Generic approach
31:39 Screening experiments
32:20 Example of screening experiment
36:51 Design of Experiments (DoE)
40:34 When to use it
41:43 Changing one factor at a time (OFAT)
43:04 Example strategy for experiments
44:40 Computer simulation and modelling
45:13 Typical modelling options
47:45 Suggested 5-Step Strategy
49:53 Summary of key points