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Yes. You are correct. You don't need to wait for 5 min. Usually loop takes to cool max 1.5 min. You can cool faster by touching/stubbing on the sterile agar surface.

In my workplace, we use disposable inoculating loop.

@@melv1n_official No problem. But it must be sterile

@@melv1n_official same here

Or you can just have extra loops not just one.

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Microbiology is very interesting. Anyways, thank you

Food science is strongly related to food microbiology. Knowing at least a little bit is essential

@@dimitrinakashidze8627 You are right

Microbiology is very essential to know about microbes as u know that microbes causes dental plaque and all oral oriented infection🤩🤩 I am microbiology student

@@sahinasapnam1704 thanks for staying with us.

We write info on the backside. But in this video we wrote on the lid to make it visible to you properly. Anyways, thanks for your suggestions.

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You are correct.

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In our lab work, we also complete streaking by burning the loop for single time. But sometimes single burning is not enough to get the isolated colony, specially for the beginner. Because they draw streaking lines roughly overlapping the lines with one another. Listen, our aim is to get isolated colonies and I can guarantee you that if you follow this method of streaking, you will must get isolated colonies on any of the streaking lines. So how can you say that it is a less efficient method where it serves our purpose? Anyways, if you are already expert in streaking, ignore this video. This video is only for the beginner. Thanks for being with us. Peace

@@MicroChemsExperiments I guess it can be for beginners. Yes I didn't knew before watching it 😂 I always manage to get isolated colonies with single burns 😅

@@MicroChemsExperiments but I meant less efficient because of the many burns needed. When I have to do like 300 petri in a day sometimes, if I lost 20min in loop cooling, I wouldn't be able to do a single day of job in a week 😂😂

@@antoine3933 You are thinking in the professional way. We also do streaking in single burn at our lab and we also know that the burning loop doesn't need 5 minutes to cool. But we made this video in this way, thinking about the beginners. Anyway, let it go. Thanks for commenting here.

@@MicroChemsExperiments I guess haha Yes yes ofc, few seconds with some "trash" petri dishes are good too 😉 Yep didn't knew my bad, have a nice evening 😁 👋

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Maybe

Different colony counter have different techniques of colony counting. Follow the manufacturer's instructions of your colony counter device

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Can u make bacterial endospore staining and gram staining.. Video

Yes. We will make

Thank uhh soo much 😊

@@aaryamohite9103 you are welcome

Not yet

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Just stub into the semisolid media targeted the bacterial colony with inoculating loop. If you get confident about that the loop touched the target colony a little then take out the loop and streak on another fresh culture plate.

We purchased the reference bacterial ATCC strain and from there we cultured the bacteria in a plate. You can also use any bacterial culture plate for streak plate technique.

We isolate bacteria from various samples in our lab

@@MicroChemsExperiments American type culture collection?

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Okay. All the best

10-30seconds is short for streaking but cooling off a loop can be gaster than 5 minutes

Yes

35-37 degree Celsius for 18-24 hours.

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Culture bacteria into Selective and Differential media first. Then go for the biochemical confirmation. You can also go for the serological test

Or on molecular based

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Great!! It is a wonderful and interesting subject. Stay with us for learning Microbiology practical.

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Because excessive heat will kill the microorganisms in that colony which is touched by the loop, thus no pure culture will be found in subcultured plate.

Noted.

Colony density will the higher in first and second quadrant streaking line, thus no isolated colony will be found. But in the third and fourth quarter lines, colony density is much lower & you will find many isolated colonies in pure form. Colony density is decreased from first to fourth quarter lines because of the frequency of burning loop. Burning loop helps to lower the bacterial cell gradually.

We will upload video tor Total Bacterial Count soon. Stay with us

@@MicroChemsExperiments Willyou use TSA for total bacterial count?

@@angelinandriamaharo7801 No. I will use Plate Count Agar. But you can also use TSA.

24 hours

Yes. It is necessary to get isolated colony

You have study more. You are the beginner. Search these words in Google and try to understand.

We will try to develop method for it later

Go for the growth promotion test using Bioball of reference culture

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Please write in english

It is needed to get isolated and separated pure colony. This method is called: 4 quarter streaking

Don't wait for 5 min. Just wait enough to cool the loop

Easy to sterilize by burning and cools rapidly after sterilizing

No,only first time you need to take smear because he was performing four flame streaking method.

Exactly

I am not sure but I think it's okay? since she/he's working on a BSC

Yes. It is okay to keep the petri dish open for the long time if you work inside the Biological Safety Cabinet. Your plate will not be contaminated. Follow my video properly. You will get isolated colony.

Great 👍

24 hours

Ok we will cut off the music from upcoming videos

Yes. You are correct

You can take a full of a single colony. Or you can just touch the loop on a colony.

You are right.

@@MicroChemsExperiments ok tell me one thing more I have culture a bacteria and pour into a test tube with fixed amount of cells. I want to incubate it once again by adding an inorganic compund into it. Shall into the same test tube, I should add inorganic compound and keep it in the incubator at appropriate temp overnight?

18-24hours

Tryptone Soya Agar, Nutrient Agar and Plate Count Agar age the universal culture media

Good Morning dear. Yes. You can use this method for fungus culture also.

@@MicroChemsExperiments Thaank you so much for your response sir, More power to you and your channel 🙏🏽❤️

@@jazzopas-iamkajorn5192 So glad to hear that

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Low bacterial cell for third and forth.

Clear the basic watching this video and take the full concept. Answering to the questions will be easier

If you are an expert then you can do this test by 2 min only

We need to touch the bacterial colony only once at the very first time. For taking bacteria. Then we don't need to dip again

من هو الاستاذ وائل؟ ماذا تقصد بذلك؟

Coliform

Yes. Add into the same tube

@@MicroChemsExperiments Two questions: 1. Is inorganic or organic compound effect the growth of bacteria. 2. When we add organic or inorganic compound in to.i think there is need to add nutrient media also so that the bacteria can grow well.please guide me. 3. If we take cells from these tubes and put with fixed amount to other four tubes along with inorganic or organic compound. Shall we add nutrient media again Please reply me.

Because you can't count all of the colonies on the plate and many colonies are burnt during burning the inoculating loop

@@MicroChemsExperiments Wooh! Thank you! That's helps a lot

A single bacterial colony have millions of bacterial cell. At the time of streaking, smear contains maximum cell number. First quarter portion will contain lower cell than smear. Second & third quarter portion will contain lower cell number than first quarter. The fourth quarter portion contains lowest cell number. Bacterial cell is decreased gradually from first to fourth quarter.

Decreasing of microbial cell is necessary to get isolated colony in most pure form

@@MicroChemsExperiments sir thank you for replying but my second question is why we are decrease colony?

@@MicroChemsExperiments thanks

@@minhasanbar1854 please knock in our Facebook page for more discussion

These two units are same. CFU/g is used for solid sample and CFU/ml is used for liquid sample

24 hours