ELISA test procedure | what is elisa assay?

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ELISA test procedure | what is elisa assay?

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Күн бұрын

Elisa test procedure - This lecture explains about the elisa test principle and the elisa test procedure. The elisa test is to identify specific antigen or antibody to detect the presence of bacterial infection. Elisa test can detect the presence of antigen or antibody in patient specimen that help us to detect bacterial infection. Stay tuned to know answers to the following questions -
What is elisa test?
Elisa test procedure?
Elisa test principle?
Elisa test classification?
How elisa test is done?
What is elisa assay?
Elisa test is also known as the enzyme linked immunosorbent assay.
Stay tuned to know the elisa test explained in this video lecture.
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Thank you for watching the video lecture on elisa test procedure and the principle of elisa test.

Пікірлер: 207

@zakiyasohana3037 8 ай бұрын

Legends are watching this video one day before exam 😅 btw....this video is very informative easy to understand helped me a lot. Thanks Shomu's biology

@shomusbiologyofficial 8 ай бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@simadassaikia8588 4 ай бұрын

Mera kal exam hain😂

@zakiyasohana3037 4 ай бұрын

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@simadassaikia8588 4 ай бұрын

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@zakiyasohana3037 3 ай бұрын

@@simadassaikia8588 From which institute?

@nilutpaldas18 Жыл бұрын

You r the warrior, our saviour. I become familiar with a topic of which I don't even know a single word and able to write it completely in exam. Thank you my teacher. ❤❤

@shomusbiologyofficial Жыл бұрын

Glad you liked it

@Taenowritesfictions Жыл бұрын

Thankyou so much sir... Tomorrow is my exam and I wasn't able to understand a single thing about this topic but you made it easier. Thankyou so much again. Your new subscriber 🌼🌼

@shomusbiologyofficial Жыл бұрын

You're welcome. Glad you liked it

@Taenowritesfictions Жыл бұрын

@@shomusbiologyofficial sir today I was able to write on it perfectly.Thankyou again sir. This video helped a lot and I recommended this to my friends too.

@b.a.i.s.h.y.a 10 ай бұрын

Same case with me

@yasminemahmoudrefaat7042 Ай бұрын

me too

@sabitasinku9548 2 жыл бұрын

Maine +3 apke video dekh kar khattam ki hai ,msc first year main hoon aur mujhe ye bhi pata hai k app ke vdo hinn mujhe pass kar sakti hai ,thank you sir 😁😁😁you are excellent 👍🏻👍🏻👍🏻👍🏻 learning from odisha

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@ebenezerandoh488 2 жыл бұрын

Hello! we're really thankful to you for all your brilliant videos about life science. you're just giving us the breakthrough into life science for free, we do appreciate that and will also support you to grow. Again, your explanation is so vivid, real in the sense that even a layman person could easily comprehend unlike others who will be just reading a downloaded ppt from google. continue.. your good work and may God be with you.

@akankshabhatt3357 2 жыл бұрын

Thankyou so much for your videos, you've helped me study for the MCAT (medical entrance exam in the US). I genuinely have learned so much from your videos and no matter wherever I am meant to head into the future based on my results, your videos always inspire me to keep on learning and expand my horizon and breadth of knowledge. God bless:)

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts. I am Honored. Stay tuned for more help. All the best for future career

@poojakumaran6734 Жыл бұрын

My favourite channel where topic is explained perfectly in a simplified way...

@shomusbiologyofficial Жыл бұрын

Thank you so much for appreciating my efforts

@AnjaliSingh-mn5qc 2 жыл бұрын

Thankyou sir. I am ug student of Biotechnology and am doing my internship in medical College and continuing this topic. You explained very well sir I have understood the whole only in one hour. Thankyou plz keep up posting.

@shomusbiologyofficial 2 жыл бұрын

You're welcome. Glad you liked it

@kamalakantakapat6768 6 ай бұрын

Now i have a confidence that i will be pass....Thank you sir 🙏

@shomusbiologyofficial 6 ай бұрын

All the best for future career

@debleenabhattacharjee6709 9 сағат бұрын

THANKYOU SO MUCH you made it COMPACT and very EASY thankyou sir

@shomusbiologyofficial 2 сағат бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@vlogtomywelcome7979 Жыл бұрын

I can clear my masters because of these types of videos by you sir.. thank you so much

@shomusbiologyofficial Жыл бұрын

You're welcome

@shreyanagpal1693 Жыл бұрын

You are indeed the best teacher!!!!!!!

@shomusbiologyofficial Жыл бұрын

Thank you

@mubarrahamjad3246 2 жыл бұрын

Very well explained! I have seen many videos on elisa before but you explained in best way .. and finall I understood the concept of this topic. Thanx alot

@shomusbiologyofficial 2 жыл бұрын

You're welcome. Glad you like it when

@kenrashi 3 жыл бұрын

Thank you sir. for making my ongoing MSC. So easy for me _💕

@shomusbiologyofficial 3 жыл бұрын

You're welcome. Glad to hear that you're getting benefit from my lectures

@pieroguastella9639 2 жыл бұрын

Thank you so much for your videos. I have just passed my biochemistry exam using your videos and i got top marks. You are the Best ❤️

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@Michela-ng4is 2 жыл бұрын

Grandeee

@rejoanakarimriana914 3 жыл бұрын

I m an undergraduate student of Biochemistry and of course so thankful to you for making our courses so easy❤ Eager to know more about your degrees and qualifications. Please share some with us so that we get motivated more. . Salam from Bangladesh ❤

@shomusbiologyofficial 3 жыл бұрын

Glad to hear that you're getting benefit from my lectures

@rejoanakarimriana914 3 жыл бұрын

You are absolutely great.. Best wishes ❤

@syeedayousufy 10 ай бұрын

I have wached many videos but i have not got the concept, like always you make my doubts crystal clear thak you so much Sir.

@shomusbiologyofficial 10 ай бұрын

Glad to hear that you're getting benefit from my lectures

@satyajyotikanjilal491 11 ай бұрын

Thank you. Very nicely explained the complicated technique in such a simple way. Best Regards

@shomusbiologyofficial 11 ай бұрын

Thank you so much for appreciating my efforts

@harriniharri1687 Жыл бұрын

This Topic became so clear because of your lecture🙏🏻🙏🏻 Thank u so much sir ☺️

@shomusbiologyofficial Жыл бұрын

You're welcome

@SG-ot4uc Жыл бұрын

You surpassed uni doctors by ur amazing explanation. Thank so much for ur efforts. Can you please download more videos for data analysis please 🙏🏻

@shomusbiologyofficial Жыл бұрын

Thank you

@risharajkhowa5060 Жыл бұрын

Sir, your way of teaching is just awesome 🙏

@shomusbiologyofficial Жыл бұрын

Thank you

@Sharvarthani 2 жыл бұрын

Such a wonderful explanation sir Thank you very much Sir ✨✨ Favourite teacher ever

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@Rohitjhilikshorts Ай бұрын

This video is very informative nd easy to understand

@shomusbiologyofficial Ай бұрын

Thank you so much for appreciating my efforts

@StellaNoire 2 жыл бұрын

Thank you so much Shomu Ji- you are such a blessing!

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@Quesofoods 3 жыл бұрын

You are educating us with a best way thankyou so much sir👌👌

@roshnibhatt2801 Жыл бұрын

Thankyou so much sir, I got very clear cut differences of all types of ELISA Test, very well explaination

@shomusbiologyofficial Жыл бұрын

Thank you

@kalaiselvi6151 3 жыл бұрын

If primary antibody has the affinity to bind to the well, then antigen vil also have the affinity to bind to the well n there is a chance of binding of secondary antibody to the antigen n gives us a colour in the end.

@gururajkalshetti198 2 жыл бұрын

Primary antibody don't have with Affinity to bind well but it is fixed in well

@padmajaofficial5863 2 жыл бұрын

Elisa plate or well is coated with blocking agent that prevents further binding or unnecessary binding

@alinasayyed9593 9 ай бұрын

Thankyou so much... watching it 3 hours before paper/

@shomusbiologyofficial 9 ай бұрын

You're welcome

@pratanupatra4006 2 жыл бұрын

Thank You Sir So Much For The Video......You Explain So Well I am a BMLT 3RD year Student and Your Videos Helped Me ALot ....Keep Sharing More Videos 🤘🏻🙏🏻🥰❤️

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@sijinmathew6299 3 жыл бұрын

Omg is this video for me👀Tomorrow i have immunology Exam🙂🌚... Plz Bless be My sir@shomus biology

@shomusbiologyofficial 3 жыл бұрын

Thank you so much for appreciating my efforts

@sijinmathew6299 3 жыл бұрын

@@shomusbiologyofficial it's my pleasure sir... Really you doing great work♥️😍♥️

@kulsumnishat1620 3 жыл бұрын

Sir you are a fabulous teacher.. Sir Please make a video on enzyme technologies too.

@shomusbiologyofficial 3 жыл бұрын

Sure. Thank you

@aamaramuzaffar3565 2 ай бұрын

Very helpful 😍 amazing

@shomusbiologyofficial 2 ай бұрын

Thank you

@xaidytff4487 2 жыл бұрын

Thank you sir, it was so clear and understandable..❤❤❤❤

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@nimramumtaz07 Жыл бұрын

Excellently explained ✨

@shomusbiologyofficial Жыл бұрын

Thank you

@sangeethabaskar9560 Жыл бұрын

Best vedio ever 💥💥💥💥

@shomusbiologyofficial Жыл бұрын

Thank you

@rezoanulfahim8237 9 ай бұрын

much of love for you my dear brother

@shomusbiologyofficial 9 ай бұрын

Thank you

@md.shirajurrahman9082 2 жыл бұрын

Great job done... Keep moving forward...

@shomusbiologyofficial 2 жыл бұрын

Thank you

@kalyanlohar476 Жыл бұрын

Thank you sir... Very nice informative lecture sir. and very excellent lecture sir... Thank you 💞💞💞👍🙂

@shomusbiologyofficial Жыл бұрын

Thank you so much for appreciating my efforts

@swathys650 2 жыл бұрын

Excellent video for elisa

@shomusbiologyofficial 2 жыл бұрын

Thank you

@Raj_02_07 7 ай бұрын

Thank you so much sir 🙏

@shomusbiologyofficial 7 ай бұрын

You're welcome

@ridamasood9878 2 жыл бұрын

Much respect from pakistan Sir.

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@divashah7920 Жыл бұрын

very very well explained sir thank u so much sir 👌👌🙏🙏🙏

@shomusbiologyofficial Жыл бұрын

You're welcome

@prajiktalonkhade1258 Жыл бұрын

Thank you so much sir

@shomusbiologyofficial Жыл бұрын

You're welcome

@subhendunayak Жыл бұрын

Thanks 🙏

@shomusbiologyofficial Жыл бұрын

You're welcome

@subhendunayak Жыл бұрын

@@shomusbiologyofficial i want lecture notes of this topic

@vishnukumart3371 3 жыл бұрын

Such a Lovely lecture 🤗

@shomusbiologyofficial 3 жыл бұрын

Thank you so much for appreciating my efforts

@syedhaya5723 2 жыл бұрын

What an explanation !!! 🔥🔥🔥

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@syedhaya5723 2 жыл бұрын

@@shomusbiologyofficial I was wandering here and there to make myself understand the actual concept, went through a lot of videos but this one cleared my concept,, keep spreading quality knowledge 🤍🤍

@Wiltflower 3 ай бұрын

during washing the unbound substance is remove from the whells😊

@sampathkumar-wx9oo 2 жыл бұрын

Superb explanation sir....👏

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts

@haroprasadpaul 2 жыл бұрын

Thank you sir

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@chaibiscooot6715 2 жыл бұрын

Hello. Can you please do a video or notes on how to calculate the reagents of indirect ELISA. Please!

@shomusbiologyofficial 2 жыл бұрын

Okay I will try

@iqrahassan3955 2 жыл бұрын

Thank you sir you are amazing 🤩

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@harishdr885 2 жыл бұрын

Sir thank you so much

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@Cupcakes74976 Жыл бұрын

Thank you so much, Sir

@hafsainam8258 2 жыл бұрын

Awesome 👏✨

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@JayAcharya-fz4kl Жыл бұрын

Sir ,I hardly request you to make one video on Immunological assay .

@shomusbiologyofficial Жыл бұрын

@_silent_eyess 2 жыл бұрын

Hello sir can u pls create another video on flow cytometry ....this video is very helpful for me and for many students ...you are truly doing great job for students who have lots of doubts and with your educational video all doubts easily cleared

@shomusbiologyofficial 2 жыл бұрын

Thank you so much for appreciating my efforts. Coming soon

@dhanrajpaulp6678 2 жыл бұрын

Hi shomu.hi shomu ❤️

@zahidaessa2336 Жыл бұрын

Sir kindly explain application.

@piyaliroy7100 3 жыл бұрын

Thanks SB...

@shomusbiologyofficial 3 жыл бұрын

You're welcome

@KalyanisVegPlanet 3 жыл бұрын

PLEASE DO VIDEO ON GMO Testing, and why it important to test GMO in food sample

@shomusbiologyofficial 3 жыл бұрын

Okay

@snehabasak4113 3 жыл бұрын

Thank you sir ☺☺

@shomusbiologyofficial 3 жыл бұрын

You're welcome

@sarthakshah6511 3 жыл бұрын

nice video

@lucygomango899 2 жыл бұрын

Sir thnkx a lot of u 🙏🏻🙏🏻🌹🌹🌹🌹 Love from...odisha 👏🏻👏🏻

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@poonamkoirala4466 3 жыл бұрын

Sir please make a video on ESBL and MBL

@BiobyNishit Жыл бұрын

sir please explain easy methods first and then go for complex methods,

@shomusbiologyofficial Жыл бұрын

All methods are already explained

@Ravifddccvf 11 ай бұрын

If you use wrong buffer or simple water than also kit will show accurate results

@iqbalsteaching2272 Жыл бұрын

Amazing teaching sir! Sir can u tell that ELISA is specific to only proteins test or it can also be used to detect any microorganism? Im not associated with this just asking for gk!

@Sweetweaponn 3 жыл бұрын

Sir please do make a video on how to prepare for GATE biotechnology 2021🙏🙏🙏

@shomusbiologyofficial 3 жыл бұрын

Okay

@sasisasi-gn7xk 2 жыл бұрын

Sir ..,we want optical immuno assay vedio

@shomusbiologyofficial 2 жыл бұрын

Okay

@niravpatel8674 3 жыл бұрын

Sir... basic peptide of tissue .... in thic topic no any one video in youtube....so please made it

@ishfaqahmad9690 2 жыл бұрын

The Direct Elisa and Sandwich Elisa is same...Correct it

@drvarshayadav5511 Жыл бұрын

sir wanted to know if the intensity of colour formed has anything to do with intensity of the disease?

@wordsforyou6542 3 жыл бұрын

Sir , you are a fabulous teacher.... Sir , please, can u make a video on the working of the China vaccine "Sinopharm".. please sir...

@shomusbiologyofficial 3 жыл бұрын

Thank you so much for appreciating my efforts

@sanjaykumar-ew6mb Жыл бұрын

Please make video on ABO DESCREPANCY IN DETAIL IN HINDI PLEASE DEAR SIR ❤❤

@shomusbiologyofficial Жыл бұрын

Okay

@dr.mohddanishstudyzaheer2558 2 ай бұрын

Your qualifications my dear

@shomusbiologyofficial 2 ай бұрын

Msc in Microbiology

@Shirmila224 3 жыл бұрын

Can U explain Geneious De novo assembly and Contig mapping ??

@gshanmugam5552 Жыл бұрын

Why serum is preferred than blood in ELISA test

@kamrunandalfaz Жыл бұрын

Sir i can't find elisa or ria incase of hormone assay which is in my syllabus...one day left for my exam...please reply here that incase of hormone how can l use the word hormone in substitute for antigen antibody... Please sir give me reply... Nobody can tell me this answer... I am waiting sir🙏🏻🙏🏻🙏🏻🙏🏻🙏🏻

@shomusbiologyofficial Жыл бұрын

I am glad

@lakshayasethi4013 3 жыл бұрын

Why will secondary antibody bind with antigen x when ag and ab follow lock and key mechanism ? when it has already bound with primary ab it shouldn’t bind with the other ab.

@aqsanaz1783 2 жыл бұрын

well, your question is very good but my point of view the answer is that although Ag Ab bind together so secondary Ab also follow the same lock and key model it does not follow any other method to bind with Ag, lock and key model does not say that one Ag Ab phenomenon always bind, does not matter how many Ag or Ab bind. Hope u got it

@nandita.spuranik7888 3 жыл бұрын

Worth watching 🙌

@shomusbiologyofficial 3 жыл бұрын

Thank you

@eleven9701 Жыл бұрын

Can someone tell me what peroxide is it ?

@abhinavjain5076 3 жыл бұрын

Very well explained. I have one question why we skip washing step in direct Elisa because we are using enzyme linked antibody and in the next step have to add substrate , substrate because product by enzyme or if antibody is not linked to particular analyte but we have enzyme linked antibody and substrate in solution so is there any chance of colour production. I am so confused please explain

@shomusbiologyofficial 3 жыл бұрын

Watch the other varieties of elisa lectures

@nehanegi6345 3 ай бұрын

In plants ELISA is different?

@dayanimitesh4786 2 жыл бұрын

Sir as you had told antibody- antibody don't bind to each other on time 12:56 in video. but in previous video of yours for topic Western blotting you told that antibody binds to antibody Fc region and shows color in solution.

@gururajkalshetti198 2 жыл бұрын

But the Fc region is already attached to well or may be both antibodies are not similar to bind each other ... As per my understanding i think that might be the reason ab doesn't bind to ab

@rameshsbiologyacademy1042 Жыл бұрын

Hi sir. Gd morning! Is secondary antibody which has linked with enzyme is similar to the primary antibody?

@shomusbiologyofficial Жыл бұрын

No. Secondary antibody binds to primary antibody and it has enzyme linked to it

@madpools_life Жыл бұрын

Why only detects protein ?

@zainabsiddique2222 Жыл бұрын

In the case of sandwich ELISA, we have a secondary antibody that can interact with a particular region of primary antibody then why after second washing and adding substrate it will not show color?

@kamrunandalfaz Жыл бұрын

Cause the substrate not individually added it is linked to the sec antibody