Fluorescence activated cell sorting (FACS)

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Fluorescence activated cell sorting (FACS)

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Күн бұрын

This lecture explains about Fluorescence activated cell sorting better known as FACS. This is a technique for cell sorting using fluorescent probe.
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Thank you for watching

Пікірлер: 68

@anythinggoes4588 7 жыл бұрын

I'm yet to meet a person on this planet, the milky way galaxy, that's completed their degree without Shomu! :) Thank you!

@shomusbiologyofficial 7 жыл бұрын

+Anything Goes glad I can help

@comoplaysdestiny5106 2 жыл бұрын

im 30 seconds in and already know this is gonna be super helpful for my technique write up paper in developmental biology. thanks!!

@TheSebastiandiego 7 жыл бұрын

Just to clarify his explanation is wrong. First the cells that have the florescence will get charged with an electrical charging ring, they are then differentiated by electrostatic deflection. they are not naturally charge they acquire a charge to differentiate the ones that do and don't have the flourecent antibodies

@igurujiaadharinstitute 6 жыл бұрын

you must know about Mr. hemant ....he is CEO OF a leading startup FLOW Jo in middle east and south east asia.....EXPERT of FLOW cytometry........MSc LIFE science fron JNU,.......PhD from max plank germany......Post Doc from chicago university..........

@subhamkarmakar3320 2 жыл бұрын

You are right

@ipeksolmaz4643 3 жыл бұрын

incredible teaching ability

@shomusbiologyofficial 3 жыл бұрын

Thank you

@ArchismanGanguly 3 жыл бұрын

You can make topic understand for everyone 😉

@shomusbiologyofficial 3 жыл бұрын

You're welcome

@sushreesangitapriyadarshin6441 8 жыл бұрын

This is how the cells get charged... The flow chamber, and the flow stream, is charged (± v where v typically lies between 50 and 150 V) at the moment a cell of interest is inside the droplet . The stream of droplets passes through a pair of charged plates (± 5000 V) so that the charged droplets are deflected and collected together with the cell container

@The9988111309 4 жыл бұрын

If a potential difference is applied on the flow chamber containing all sorts of cells then the whole stream of droplets will be charged right? Why only the cell of interest? The more complex way is that the cell of interest is charged based on the scattering where the pattern is detected and when the flow stream exits forming droplets at the bottom then they are charged accordingly with an electric ring present, then they are separated with the help of charged plates in the end.

@carolinegronnier6888 4 жыл бұрын

Thank you so much for sharing this really clear video

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@mayukhroy5393 4 жыл бұрын

This video was too helpful Sir.

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@isabellajung9834 Жыл бұрын

Thank you very much for this good explanation! I have one question: Where exactly is the difference between die amount of proteins and the number of cells expressing this protein. Is this about how much of this proteins are on the cells, so more about the quality?

@ravianandrao7761 8 жыл бұрын

Thank you so much shomu bro...

@hemantagrawal3772 9 жыл бұрын

My dear there is no use of radioactivity in flow cytometry. Please correct your sentence. We use fluorescent proteins or dyes but no radioactive material

@pranjaldhaka435 8 жыл бұрын

+Hemant Agrawal so FACs just record the fluorescence of the tags or flourochromes attached to the cells right?

@hemantagrawal3772 8 жыл бұрын

+Pranjal Dhaka In flow cytometry, cells or cell like particles can be analyzed based upon their physical properties like cell size and cell complexity plus cells of interest can also be identified in a mixture by tagging its specific markers (surface/intracellular) by antibodies labeled with fluorescent tag. Sometimes cells can also be studied using fluorescent dyes without any antibodies for example viability assay, side population determination etc. Please see a link, where flow cytometry is explained in a very nice way www.d.umn.edu/~biomed/flowcytometry/introflowcytometry.pdf

@igurujiaadharinstitute 6 жыл бұрын

PRANJAL DHAKA you must know about Mr. hemant ....he is CEO OF a leading startup FLOW Jo in middle east and south east asia.....EXPERT of FLOW cytometry........MSc LIFE science fron JNU,.......PhD from max plank germany......Post Doc from chicago university..........

@Captainlab01 4 жыл бұрын

Great to see u Sir

@kusfierce4328 3 жыл бұрын

Sir ..you r always ..a saviour ... Love u sir .. u make the topic soo easy to get understand ... ❤️❤️ Love from heart

@shomusbiologyofficial 3 жыл бұрын

Thank you so much for appreciating my efforts

@franciscoperez6132 4 жыл бұрын

Excellent explanation, salam amelicum❤👍

@shomusbiologyofficial 4 жыл бұрын

Thank you

@kalpanajaiswar5025 2 жыл бұрын

Thank you so much sir 🙏🙂

@shomusbiologyofficial 2 жыл бұрын

You're welcome

@smarakipattnaik685 2 жыл бұрын

Sir, how many cells can be sorted by charging method in this technique ?

@rezafarahmand2695 Жыл бұрын

Thank you,good for you👏👏👏

@shomusbiologyofficial Жыл бұрын

You're welcome

@vgreddy_velma 4 жыл бұрын

very useful for beginners

@shomusbiologyofficial 4 жыл бұрын

You're welcome

@risharajkhowa5060 Жыл бұрын

thank u,so much Sir

@shomusbiologyofficial Жыл бұрын

You're welcome

@sinamt2982 Жыл бұрын

How do the different cells get the different charges to seperate them in the electric field?

@ullaskotihal1911 5 жыл бұрын

Thanks bhai Fan from Karnataka 😍 We wanna meet you

@shomusbiologyofficial 5 жыл бұрын

You're welcome. Glad to hear that you are getting benefit from the videos

@ullaskotihal1911 5 жыл бұрын

Where are you from bhai?

@gubeshgunaratnam4615 7 жыл бұрын

very nice explanation

@ankitalankar6042 3 жыл бұрын

thank you sir

@muhammadahmad-ft7fv 7 жыл бұрын

awesome job bro ,,,keep it up ..ALLAH bless u

@soniyavincent9116 3 жыл бұрын

May i know that is this answer for flow cytometry and fluroscence

@vijetasingh416 5 жыл бұрын

Sir please give lacture on the quenching fluorescence and phosphorescence please

@eggnogs 6 жыл бұрын

Having used the FACS machine for multiple years, I can verify that this information is incorrect. Also fluorescence is not radiation. You need to go back to school and pay attention

@shibrajpradhan6849 8 ай бұрын

😂 something is burning

@avinashkumargudadurkalmath7368 Жыл бұрын

R u with me I m going to xenotransplantation process practically from monkey genes to human tell what are the steps involved in that

@wonderfulIndia1 4 ай бұрын

Sir flow cytometry bhi ise hi bolte h kya sir plzz reply

@shomusbiologyofficial 4 ай бұрын

Yes

@rijilfreekdaredevil Ай бұрын

Thank u sir

@shomusbiologyofficial Ай бұрын

Welcome

@hosseinghadamyari3513 8 жыл бұрын

very good

@pamelajulia1610 3 жыл бұрын

The intensity is on the x axis. Not y

@surajsaksena 8 жыл бұрын

totally wrong explanation of sorting..

@shomusbiologyofficial 8 жыл бұрын

+Suraj Saksena really? Please explain your points

@2lefThumbs 7 жыл бұрын

I explained quite clearly where you were wrong over a year ago, and you deleted my reply, if you leave this intact, I'll gladly reconstruct my critique

@mercy9386 4 жыл бұрын

@@2lefThumbs UMMM can I please get the correct explanation then please? I need it ASAP

@love.vibe.official 6 жыл бұрын

GAL4/UAS.... Please ..... Upload.. This topic

@noorsbiology7580 3 жыл бұрын

ur x and y axes are switched I think

@atulanand7993 7 жыл бұрын

Wrong.....👎🏿

@MarceloZerillo 8 жыл бұрын

Hi Suman, I have a question. I am reading a paper in which FACS was used and for a control of the experiment a batch of the cells was treated with 70% ethanol in order to kill them. They report the counting of the dead cells, but I wonder if it is still possible to detect those dead cells using FACS, since the membrane would be disrupted and the cell content would be lost. Any guess? Thank you.

@pranjaldhaka435 8 жыл бұрын

+Marcelo Zerillo I think the purpose of the control in the experiment was to show the relative inability of the fluorescent tags to attach to the cells which got destroyed due to ethanol treatment. It still depends on the fluorescent tag used though because some tags attach to a specific protein under specific conditions,.

@hemantagrawal3772 8 жыл бұрын

+Marcelo Zerillo In Flow cytometry, people use viability dyes like PI, 7AAD, DAPI etc to detect dead cells. The principle is that dye gets bind to nucleic acids once the membrane is compromised and we can easily differentiate between dead and live . It has nothing to do with the non-attachment of fluorescent tags to the dead cells. Please see this link for a flow figure www.uvm.edu/medicine/flowcytometry/documents/ViabilityusingPI_000.pdf

@MarceloZerillo 8 жыл бұрын

+Pranjal Dhaka thank you so much for your answer.

@MarceloZerillo 8 жыл бұрын

+Hemant Agrawal thank you so much for your answer.