The Microscope Upgrades We've Made Along The Way

The Microscope Upgrades We've Made Along The Way | Compilation

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2 жыл бұрын

Go to microcosmos.store before August 8th to get 10% off of almost everything in the store and to pick up a limited edition Dark Mode Hydra T-Shirt!
This channel wouldn’t be what it is if it weren’t for one very key invention: the microscope. Everything we see, we see with the aid of light and lenses, expertly deployed by our master of microscopes, James. And if you’ve been on this journey from the beginning, or if you’ve ever gone back to revisit our earlier videos, you may have noticed that things have changed a bit around here.
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SOURCES:
micro.magnet.fsu.edu/primer/a...
micro.magnet.fsu.edu/primer/a...
www.geog.ucl.ac.uk/resources/...
micro.magnet.fsu.edu/primer/t...
micro.magnet.fsu.edu/primer/j...
www.microscopyu.com/technique...
micro.magnet.fsu.edu/primer/t...
www.microscopyu.com/technique...
www.microscopyu.com/technique...
www.microscopyu.com/tutorials...
www.microscopemaster.com/diff...
www.microscopyu.com/technique...
www.microscopyu.com/microscop...
www.olympus-lifescience.com/e...
www.microscopyu.com/tutorials...
www.microscopyu.com/microscop...

Пікірлер: 204

@journeytomicro 2 жыл бұрын

Go to microcosmos.store before August 8th to get 10% off of almost everything in the store and to pick up a limited edition Dark Mode Hydra T-Shirt!

@Bc232klm 2 жыл бұрын

Second :)

@popCORNcandy Жыл бұрын

The store looks so wonderful. Visually speaking. Hope I will get a microscope from there soon. By the way it will be really great if we have an interview with James where Hank will be the host. Most probably I am not the only one who desires it sooo badly!! Greetings to Journey to the Microcosmos team.

@herbertnatanael Жыл бұрын

Helo

@SeaJay_Oceans Жыл бұрын

God is Great ! Life is GOOD ! :-)

@boymeetsdrake6438 Жыл бұрын

the channel starts slowly becoming more about james then about the microbes lol

@agnorat 2 жыл бұрын

It’s 2:27 am here in Aus. And this is exactly what I need Mini lads with James n hank

@sycration 2 жыл бұрын

🦠mini lad

@CaptainCandycorn Жыл бұрын

1:49 am here, same. Chillin with the micro bois.

@ianmccann Жыл бұрын

3:33 here

@user-rp4et4hc4z Жыл бұрын

Same 2w later hahaha as soon as i read the comment

@anadipatel8545 Жыл бұрын

nice

@dr.jayburness6522 Жыл бұрын

It would be great if James could do a video on his workflow from his technique for wet slide preparation to video recording.

@matthall8744 Жыл бұрын

When I first saw the channel I wondered about the instruments used. This is a fantastic explanation of the technology itself. Thank you James & team.

@Zunderfeuer 2 ай бұрын

Me watching this video, trying to rekindle my love for using my for me expensive enough little microscope (400.-) and seeing his type of microscope model going up for 35.000.- from Zeiss :-o God it is so hard not to compare yourself with this kind of stuff.

@obieobrien5883 2 жыл бұрын

It’s always fascinating to watch these videos! Teaching urinalysis, my students were excited to see what was in it. There were so many things they identified. Crystal shapes was always the most fun. It wasn’t just liquid anymore.

@rainstorm_jo Жыл бұрын

MLS here - my favorite crystals are calcium oxalate monohydrate form. I discovered on my own that they polarize. They look similar in size and shape to RBCs, which do not polarize. also I love seeing motile bacteria =)

@SeaJay_Oceans Жыл бұрын

People gaze down at the tiny critters and are stunned by their beauty... Then they look up to the Stars and Galaxies, and remember we too, are tiny critters ! :-D

@AR15andGOD Жыл бұрын

@@SeaJay_Oceans that's not what determines something being small

@SeaJay_Oceans Жыл бұрын

@@AR15andGOD said the teeny tiny bug stuck to an itzy bitzy mud ball planet floating around an insignificant yellow star... ;-)

@JimmyKnax Жыл бұрын

@@AR15andGOD Actually, I feel that comparison between objects and/or levels in systems is exactly how one goes about determining something is "small". Everything is relative to everything else

@nav7icula Жыл бұрын

This was a very nice and visually interesting journey of your group's progress in photographing the aquatic micro-world. The new Zeiss microscope was money well spent for your teaching abilities. However, tell James that the excellent picture of his rotifer in 36:03 to 36:30 is not a Keratella. It is a closely related loricated rotifer called Lepadella; probably Lepadella ovalis.

@Bolt6265 2 жыл бұрын

Yknow I've always wanted to see a microscope that can somehow do like a lightfield in realtime so the entire depth can be in focus simultaneously because the most annoying thing about microscopy is the incredibly shallow DOF and constantly having to adjust focus.

@SeaJay_Oceans Жыл бұрын

There is a software based digital camera that can do exactly as you say - I forget the name of it, but it basically gives you 100% DOF by processing all of the captured light and focusing all of it (computationally) so that the final image is ALL crystal clear. Not exactly 'real time' but very close, a few miliseconds delay as Billions of circuits transform data form one form to another, and produce for you, the detailed image for your analog eyes!

@benmcreynolds8581 Жыл бұрын

I totally agree. When I got into really up close macro photography I learned about just how shallow depths of feilds can get for different lenses or how your lense works with your sensor of your camera. So ya it would be great to see like a triple or quad stacked layer machine that puts all the depths of feilds into one continuous image.

@carbonium1264 Жыл бұрын

@@SeaJay_Oceans do you mean LYTRO ? yeah light field cameras are AWESOME 😍😍😍

@SeaJay_Oceans Жыл бұрын

@@carbonium1264 Ya, i think that's it - a digital camera that captures ALL the light and the focus is all done in software...

@potatofuryy Жыл бұрын

@@benmcreynolds8581 Yeah. I love macro photography, It’s so cool to see ordinary creatures in higher resolution than your own eye can resolve.

@RallySelf 4 ай бұрын

Thank you for your videos. I lost my uncle today and your videos have given me a calm and a piece in this moment in this day that I could explain but I just want to get back to watching micro. so just thank you. My uncle would have liked you too.

@andrewmontgomery1763 Жыл бұрын

I love your content! I'm a grad student currently, and we spinning disk confocal microscopy to visualize fluorophore tagged proteins in living neurons, so we can see how and where they traffic in the cell.

@franzferdinand1782 Жыл бұрын

I’m a lab intern and my favorite thing is the confocal lol, it feels like flying a spaceship. We do retinas!

@andrewmontgomery1763 Жыл бұрын

@@franzferdinand1782 That's really awesome, and same feeling as well!

@SoleSolSoul Жыл бұрын

I have never learned more about light scientifically, all at once and so simply, than here. And I’ve studied photons, waves, energy quite a bit.

@Q8Bart 2 жыл бұрын

Microbes can make selfies now ;)

@ktl4539 Жыл бұрын

Very well presented. When the micro-world was introduced in middle school, that was when I knew I was going to be a scientist. I hope this inspires today's students...👍

@ibanix2 Жыл бұрын

“If you have ever taken an optics class… you will remember how terrible that was” - as a physics major I feel this personally

@huntermaverickwells7289 Жыл бұрын

Hank you're the next david attenborough. phenomenal VO work, amazing documentary here

@benroberts3677 2 жыл бұрын

The job I work at is very sad, and not at all what I want to be doing, but seeing this video in my notifications pushed me through today.

@andrewmontgomery1763 Жыл бұрын

What's your job?

@xafierah 2 жыл бұрын

Hey! Optics class wasn't THAT bad! (Love the descriptions of the methods while comparing the video footage - really nifty!)

@1mcob 2 жыл бұрын

Totally awesome.... as an amateur, this was a helpful overview.

@MBY1952 Жыл бұрын

כל הכבוד על הפרויקט. תודה רבה.

@fintux Жыл бұрын

15:42 - I didn't know I've been occasionally looking at microscopic DIC pics. The things you learn watching this channel!

@evelyne7071 Жыл бұрын

So much information……..so little time. Thank you for the explanations of the different filters and their effects on light.

@BTheBlindRef Жыл бұрын

I just gave a compilation video a thumbs up, and that is something I never do. But this one is the rare examples of where a compilation video was absolutely the right thing to do (short of filming an entire new video that encompasses the entire combination of scripts).

@sneakysquirrl708 Жыл бұрын

Your videos bring me joy 🥹 thank you.

@sabrinafelber Жыл бұрын

Wow! Fascinating!

@Geekchess 12 күн бұрын

A very important video. Cheers for all the information you've provided within it. 🍻

@mrcrazyadd2 2 жыл бұрын

4:50 I didn't appreciate that rotifer flipping me off

@-Oleg1 Жыл бұрын

Greatest video i've seen this year. THANK YOU!

@DumbBaby 11 ай бұрын

This channel is absolutely amazing to me, thankyou:) I love watching the episodes or just projecting the footage while making music to put me in a good space. Are there are any more collections of just footage out there? I've looked but can't really find any

@CrispyGFX Жыл бұрын

Is there a way to capture an image with multiple processes to get a compound image, similar to how telescopes can combine IR, visible light, etc., to resolve a more 'broad' image?

@stevebluh Жыл бұрын

I remember back when I didn't know it was Hank doing the voice over, he sounded like he didn't want to scare away the microorganisms. Good times.

@alberto148 Жыл бұрын

Q: what does a microbe actually look like? A: Laurence Fishburne: do you think that's air your breathing? gee thanks hank.

@jacobwoodbury6073 2 жыл бұрын

Wow, thanks for sharing... this is really interesting and inspiring, science is cool

@glennz8273 Жыл бұрын

This is a magical episode ❤

@HanifAhmadFauzi Жыл бұрын

That's amazing. Great jobs!! 😎👍

@BoydRB3 2 жыл бұрын

Amazingly clear images! Do you have any tips on how to keep your optics free of dust?

@JamsGerms Жыл бұрын

I built a cat tower over mine! -James

@LeonVonDai Жыл бұрын

James is killin it!

@Nikkes02 2 жыл бұрын

Congratulations on the new microscope :D

@napoleonhardin7954 Жыл бұрын

Absolutely amazing. Wow! Super cool. 👍🏾😁

@DaRios_Tristan Жыл бұрын

what a wonderful journey❤😊

@sarahlynn7807 Жыл бұрын

Amazing footage!

@Spacecomber 2 ай бұрын

I enjoyed your video and your presentation of how the optics involved can enhance what can be seen through a microscope. One thing that I might have given a bit more attention to is how limited the depth of field is under a microscope’s high magnification. It tends to create the impression that microorganisms are relatively flat, when in fact we are seeing a cross section of a largely transparent object. We learn to compensate for this by shifting the focus up an down a tiny bit, essentially trying to create a an imaginary “stacked image” in our mind’s eye of the organisms more 3-dimensional shape. Very few of will ever get the chance to use the most sophisticated optics, such as DIC, to observe that 3-dimensionality more directly. But, for most of the history of the study of microorganisms, a bright field microscope and an artistic talent provided the content for our textbooks.

@benmcreynolds8581 Жыл бұрын

This is amazing. Is it possible to see bacterial phages? I saw a video going into the study of all sorts of types of phages and the possible uses of them if studied correctly. The scientists would go around and collect water samples in weird areas to find different phages that specialized in different things. Is that a possibility for you guys to do ever? Just curious?

@pilotavery Жыл бұрын

They're microscopic even to a microscope. Some of them are only 200 atoms wide.

@omersar4221 Жыл бұрын

people should watch to learn more on youtube. Your effort and also teaching style increadible. i found this channel while looking something to learn and you deserve the best. thanks for all effort.

@vernonbrechin4207 2 жыл бұрын

Though lengthy this was wonderful. I finally became a Patron of your channel. I will be looking forward to the improvements of James's equipment. I'm glad the other producers are also evolving into this hobby. I was impressed that these productions avoid employing stains that might alter the behavior of this amazing world.

@glennbabic5954 Жыл бұрын

Love that T-shirt print. I had to buy one. First thing I've liked so much to buy from a KZfaqr store

@cindyclay1750 Жыл бұрын

Wow! 😍 So wonderful to see Nature's little surprises w/o "staining"! 😆 Your lighting brings out the eye candy of delights! 😍Thank you!! 💋

@Ngoctiennguyen2307 9 ай бұрын

Thanks so much ! Master

@NewMessage 2 жыл бұрын

Well... Time to make some popcorn, I guess.

@nicoloparacini633 Жыл бұрын

We love u James

@caballarius503 2 ай бұрын

is there somewhere we can find all your setup including cameras, objectives, everything that you use for your videos

@susannahallanic1167 11 ай бұрын

Thank you!

@garycroftsmicroscopy Жыл бұрын

Amazing quality

@gilly68g Жыл бұрын

Are you ever going to go to superresolution or get down to the molecular scale? What molecules are involved and what are they doing? PSI, PSIi, antenna proteins for instance. Yes the videos are awesome and beautiful but getting into the Why? Of what is giving rise to the colored structures could really inspire a lot of the viewers to pursue the deep molecular science behind the images. Maybe bring a structural biologist into the mix? Love the footage!!

@SaronJoy 9 ай бұрын

It's all about perspective. Microscopes look down onto/into the microbial world, a real "birds-eye view". I think about us humans being viewed just from above. It's not until you come down to our level and face us, that you get a true understanding of what we look like. I can't wait until someone invents a Microscope whose optics peer directly at microbial life on their level... something akin to a side-microscope. Until then, we can only imagine.

@bigbrady2244 Жыл бұрын

what great footage, i thought this one was edited abnormally well too.

@ericccowan Жыл бұрын

Which camera are you guys using on the new scope?

@philipchesleyiii Жыл бұрын

What camera do you use for dark field? I have a camera that works good in bright field but in dark field it gets real bad motion blur.

@t.properties6878 2 жыл бұрын

I just ordered yesterday. Dad burn it! Got the whole setup.

@SJ.1988 Жыл бұрын

Speechless.

@akbblessed1415 11 ай бұрын

What is the model of the phase condenser? What is the model of the microscope? Etc etc? We need detailed information please. Thanks

@pheart2381 Жыл бұрын

I found my first tardigrade 2 days ago using a pre-1880 Brass drum microscope.sometimes less is more!

@Austin_Nova 2 жыл бұрын

could you guys start uploading in HDR that would look stunning

@jonathanleonard1152 Жыл бұрын

You had me wanting to touch the computer screen during the DIM section.

@nunyafunyuns 7 ай бұрын

You can buy all the equipment you like, it ain't nuthin til James points it at sumthin.

@napoleonhardin7954 Жыл бұрын

Thanks for the upgrade, guys. 😁

@cracko4358 Жыл бұрын

Aaaaaa yes this is the sleep I needed

@brianazmy3156 Жыл бұрын

Rather impressive I must admit

@anticat900 Жыл бұрын

I found my 60x objective on my cheaper microscope to be almost unusable even with oil. It was just close to the slide. Yet here you are using 100x, and getting great images. Do you use special extra thin slides to enable this?

@scottthomas5819 Жыл бұрын

Yesss

@fabricebec5972 Жыл бұрын

thank you

@CHKNSkratch Жыл бұрын

Here's a random question: if I were shrunk down to the size of a bacteria, how difficult would it be to punch through the membrane of a bacteria?

@ESF19791111 Жыл бұрын

WAW...AMAZING VIDEO ( ONE MORE TO THE AMAZING VIDEOS COLLECTION :) THANK YOU FOR EXPLAINING ABOUT THE MICROSCOPE WORKING TECHNIC :) AND IM GLAD FOR THE UPGRADES :) THANK YOU FROM ISRAEL :)

@nawapolachawanantakul5850 2 жыл бұрын

Beautiful

@kurt7020 Жыл бұрын

If they make such a thing: Real time `focus stacking` for microscopy. It'd be pretty slick.

@SeaJay_Oceans Жыл бұрын

@michaelthomasdixon2031 11 ай бұрын

What's a good starter microscope.

@polytrichum1119 Жыл бұрын

Cool video!

@SolveEtCoagula93 Жыл бұрын

Love the comment, 'If you've ever taken an Optics course - you will remember how terrible that was!'. - Oh yes - and some more! The problem was that first time around, it all just didn't make any sense. For me, it took years, gradually refining my knowledge until finally I came to appreciate just how incredible the whole subject is - and the maths is mind-blowing. Sadly, as a Physics student, I never got to see images like these, but I did eventually work with neurons and would so love to see them via these techniques.

@jennifermarshall-craig5146 Жыл бұрын

I would love to see what some parasites (I.e. Giardiasis) would look like using the different microscope lighting techniques

@yaza558 8 ай бұрын

i want one, please, produce more :)

@barbarahouk1983 Жыл бұрын

This review highlights the facts of how our senses work. The microscopes extend the human sense of light images upon the compound receptors we call the eye. I am a retired psychiatrist (MD), who has been fascinated by the human as a machine as well as a spiritual being. How a human learns is just one of the many aspects that has kept my attention. All learning is done by comparison. The human does not see what exactly is. The human brain builds upon comparisons. The goal is to get a representation as close to reality as possible. Reality is that which exists beyond one's receptors (senses). Often a mistake can be made in interpretation of stimulus; this is called an illusion. So with the microscope and the different techniques, one can get different views. This information has to be integrated. Integration is what the human mind uses to understand the environment. The closer to reality is the goal.

@TedToal_TedToal Жыл бұрын

How about photographing in shorter wavelengths, such as far UV, and translating the image into colors we can see, like astronomers do. Is that done? How about simultaneously recording data at a variety of magnifications, at a very high sampling rate, and composing a database that can be explored in VR to visit and revisit different aspects of the organism?

@stephenchallener9824 Жыл бұрын

You can do higher res imaging with UV vs visible light but it is challenging (even aside from obvious safety issues) because most glasses absorb UV. You basically need every lens and bit of glass in your system to be made of specialized materials.

@TheZombie2415 Жыл бұрын

We need to go upgrade further

@ivanfedak4517 2 жыл бұрын

❤❤❤ and no more words

@1estab41 Жыл бұрын

ca i suggest samples from aquaculture industry such as shrimp culture (vannamei). more of the concerns are protozoa, vibrios and viruses,

@TedToal_TedToal Жыл бұрын

I just recalled that each and every cell of our bodies is off similar (or more?) complexity to one of these complex single-called organisms. Mind-boggling.

@wheresmykarambit7859 2 жыл бұрын

Early to the Microcosmos, let’s gooooo

@SumAkwardDude 7 ай бұрын

What microscope is this pls

@mastershooter64 Жыл бұрын

can't till james gets his hands on a electron microscope

@Shadowfax2121 2 жыл бұрын

You guys really ought to do a collaboration with "Matt Powers - The Permaculture Student" - He is researching soil based microbes and fungi in an attempt to improve agriculture and gardening practices. He covers a lot of microbes and I think your channels could really benefit from working together.

@NCRonrad Жыл бұрын

Second this !

@pharaohsmagician8329 Жыл бұрын

That's awesome. People who believe they can change the world and do things like that often can. I want to discover something cool too, but truthfully only as a get rich scheme. And I think that money only mindset prevents yourself from devoting, or even starting, down the path of personal research into hobbies that interest you. A lesson for myself to not only think of things for money, because it might stop me from using passion to find something that I can then sell and make lots of money 💸💰 haha

@MrTimjwilson Жыл бұрын

Really? I did not see that Powers stuff even comes close.

@luisito6314 Жыл бұрын

This is so much more interesting than big creatures

@TedToal_TedToal Жыл бұрын

Is computed tomography ever used in microscopy? It seems like we’re missing so much because we’re only seeing things in 2D. What if we could project a hologram of the thing we were viewing out into the air in front of us and walk around it and inspect it?

@Mikeb8134 Жыл бұрын

yes

@TedToal_TedToal Жыл бұрын

Is spectroscopy ever used in microscopy? Might not be possible to learn a lot more about an organism by looking at the spectrum of light emitted by each part of the organism when it is illuminated?

@luisito6314 Жыл бұрын

Can you check out whats happening in blood while still in the body?